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R Saxod

Publications and source records attributed to R Saxod.

50 records · Page 3Linked to original sources
PubMedDate not supplied

There is not simple method to maintain a constant ethanol concentration in long-term cell culture: keys to a solution applied to the survey of astrocytic ethanol absorption.

Ethanol evaporation from the culture medium is a potential source of misinterpretation of long-term exposure of cells. Different methods have been proposed to prevent this evaporation, the most effective being the saturation of the atmosphere over the culture medium with ethanol. Unfortunately, no simple predictive method has been devised to determine the appropriate concentration of ethanol in the system avoiding either evaporation or contamination of the culture medium. We present some keys to a solution adapted to the culture of astrocytes, which allow for the first time a direct evaluation of ethanol absorption by these cells. The system described remains compatible with normal growth and viability.

Absorption↗
PubMedDate not supplied

Quantitative analysis of growth and myelination of cutaneous nerve fibers in the chick.

Cutaneous thoracic nerves from 16-day embryos to 6-month chickens were studied. Special attention was given to the increase in caliber of developing axons, number of lamellae in the myelin sheath and distribution of group sizes. Myelination begins at 14 days of incubation but occurs mainly after hatching: 5% of fibers are myelinated in the 3-day chickens, 20% at 16 days and 63% at 6 months. During development, a rectilinear positive correlation between the axon diameter and the number of myelin lamellae was found at all stages, except in 16-day embryos, and the slope of the calculated regression lines decreased with age, as mean diameter and sheath thickness increased. The rate of daily myelin initiation was maximum between 3 and 16 days after hatching while the speed of myelin formation decreased with development. For nonmyelinated fibers, a regular reduction of the number of fibers per Schwann cell takes place during nerve maturation.

Animals↗