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Biomedical subjects

R Sergio Pasqualini

Publications and source records attributed to R Sergio Pasqualini.

7 recordsLinked to original sources
PubMed2002

Birth of a healthy baby after transfer of embryos that were cryopreserved for 8.9 years.

OBJECTIVE: To report successful pregnancy from embryos that had been cryopreserved for 8.9 years. DESIGN: Case report. SETTING: Fertility clinic of the University of Buenos Aires School of Medicine. PATIENT(S): A 43-year-old woman with ovarian failure and a 45-year-old man with nonobstructive azoospermia underwent embryo donation.I NTERVENTION(S): Five embryos that had been cryopreserved and stored since 1989 were thawed in 1998 and donated to an infertile couple. Endometrial preparation was performed with 17beta-estradiol and progesterone. The four embryos with better morphologic characteristics were transferred into the uterus. MAIN OUTCOME MEASURE(S): Post-thaw embryo survival, pregnancy, and birth. RESULT(S): Embryo survival was satisfactory as assessed by morphology. Pregnancy was confirmed by ultrasonography. A healthy baby weighing 2120 g was delivered by cesarean section at 36 weeks. CONCLUSION(S): Human 8-cell embryos may be viable after extended storage and can result in successful pregnancy.

Adult↗
PubMed2002

Birth of two babies using oocytes that were cryopreserved in a choline-based freezing medium.

BACKGROUND: Oocyte cryopreservation may have significant potential for assisted reproductive technology. However, to date, successful results have been limited. We report a preliminary series of IVF outcomes after fertilization of oocytes that were frozen in a low-sodium medium. METHODS: In this retrospective analysis, 12 patients (21-41 years old), who underwent IVF in a fertility clinic affiliated to the University of Buenos Aires, had oocytes cryopreserved in a modified phosphate buffered saline medium, in which sodium chloride was replaced by choline chloride. A slow-freezing, rapid-thawing protocol was used and oocytes were inseminated by ICSI. Outcome measures included oocyte survival, fertilization, implantation and pregnancy rates. RESULTS: Median oocyte survival was 63%. Median fertilization rate was 59%. Overall implantation rate was 25%. Six clinical pregnancies were achieved; two of these pregnancies went to term resulting in the birth of two babies. CONCLUSIONS: To the best of our knowledge, these are the first pregnancies and normal births using oocytes that were cryopreserved in a choline-based medium. The small sample size prevents us from concluding that freezing in a low-sodium medium is superior to using a conventional one.

Adult↗
PubMed2000

[Hypofibrinolysis and other hemostatic defects in women with antecedents of early reproductive failure].

The fibrinolytic system is involved in blood clot lysis and in other biological processes that require extracellular proteolysis such as ovulation, blastocyst implantation and trophoblastic invasion. An impaired fibrinolytic capacity is a common feature in women suffering from unexplained early recurrent pregnancy loss. We carried out a prospective study with 114 patients. Three groups were established: Group 1: women who had two or more unexplained miscarriages of < 12 weeks of gestation (n = 52). Group 2: women who had 2 or more embryo-implantation failure after embryo-transfer with good quality embryos in assisted reproduction (n = 46). Group 3: women who had 2 or more embryo-implantation failure after embryo-transfer and one early loss of a pregnancy achieved in a cycle of assisted reproduction (n = 16). Fibrinolytic pattern pre and post-occlusion test was carried out: ECLT, PAI-1i, PAI-1b, t-PAi, t-PAb. In addition other studies were performed: LAC, ACA IgG and IgM, fibrinogen, factor XII, APCR. A high prevalence of thrombophilic defects and hypofibrinolysis was observed in the three groups. The fibrinolytic pattern was similar in the three groups, although a prolonged pre occlusion ECLT was more frequent in women with primary infertility (Group 2). Isolated hypofibrinolysis was found in 50% of the patients. The remaining patients with hypofibrinolysis presented combined defects with LAC, ACA and abnormal APCR. The fibrinolytic pattern in women with or without antiphospholipid antibodies was similar, although an elevated pre occlusion PAI-1i was more common in women without antiphospholipid antibodies. High prevalence of fibrinolytic abnormalities observed in women with unexplained early recurrent pregnancy loss and in women with repeated failure implantation after embryo-transfer in assisted reproduction, suggests that hypofibrinolysis could be a parameter of early reproductive failure.

Abortion, Spontaneous↗
PubMed1995

[Pregnancy with testicular spermatozoa].

In a patient with a diagnosis of congenital vas deferens agenesis we decided to carry out a microsurgical epididymal sperm aspiration in order to attempt an in vitro fertilization (IVF). Since during scrotal exploration no epididymus was found (probably as a consequence of a previous surgical biopsy) a testicle biopsy was performed. The latter yielded a few hundred sperms which were injected within the cytoplasm of his wife's oocytes (ICSI technique). Of the 18 oocytes, 3 were harmed during the procedure and 16 hours later signs of normal fertilization were detected in 6 of them, followed by the development of 4 embryos. These were transcervically transferred to the uterus, 3 in 8 cell stage and 1 in 6 cell stage; previously a 20 micron hole had been made with acid Tyrode solution in the pelucida zone of each embryo. Fifteen days later, the result of the HCG beta subunit assay was positive and a month later a single 19 mm normal embryo was detected by ultrasound echography.

Adult↗