Biomedical subjects
Publications and source records attributed to R Serrentino.
The Authors, after a short review on the histopathological, histogenetic and prognostic aspects, report a clinical case observed by them, in a pregnant woman. They emphasize the asymptomatic obstetric course and the accidental discovery during surgical treatment.
A personal observation of an uterine myoma in teenagers led the Authors to evidence the exceptionality of this clinical case. With regard to therapy, myomectomy is the most effective treatment.
In the present paper, a simple photometric assay of microsomal epoxide hydrolase activity using the 4-NBP test is described. The outline of this method is the reaction of 4-NBP with the unreacted oxide extracted from incubation medium in separated ethyl-acetate layer, followed by measurement of absorbancy at 560 nm. The usefulness of this assay can be in application to all epoxides enough alkylating and it can be used for routine analyses which don't request either particular instruments or suitable standards of synthesis.
The suture of episiotomy is certainly a problem in modern obstetrics. The Shute seam, still largely unknown in Europe, is a good technique and offers a much cleaner wound healing and a significant reduction of pain during childbed with very satisfactory functional and cosmetic results.
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The Ks values from type I binding of several aliphatic epoxides to microsomes from phenobarbital-or 3-methylcholanthrene-pretreated or untreated mice have been determined. A good correlation between binding affinity and substrate lipophilicity (as log P octanol/water) was observed. Moreover, a striking correlation between delta Emax and log P was found, pointing to a great importance of lipophilicity for the interaction of these compounds with hydrophobic membranes-bound cytochrome P-450. The Ks values of some parental olefins were determined and found to be of the same extent as those of respective epoxides. This suggests that the epoxides can be still good substrates for the mono-oxygenase. The resulting possible hydroxylation would be a further way of detoxication process. This is also supported by the fact that epoxides inhibit the aminopyrine N-demethylase activity.