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Biomedical subjects

R Simpson

Publications and source records attributed to R Simpson.

At least 109 records · Page 6Linked to original sources
PubMed1985

Pelvic tilt. Intratester reliability of measuring the standing position and range of motion.

The purpose of this study was to examine intratester reliability of a test designed to measure the standing pelvic-tilt angle, active posterior and anterior pelvic-tilt angles and ranges of motion, and the total pelvic-tilt range of motion (ROM). After an instruction session, the pelvic-tilt angles of the right side of 20 men were calculated using trigonometric functions. Ranges of motion were determined from the pelvic-tilt angles. Intratester reliability coefficients (Pearson r) for test and retest measurements were .88 for the standing pelvic-tilt angle, .88 for the posterior pelvic-tilt angle, .92 for the anterior pelvic-tilt angle, .62 for the posterior pelvic-tilt ROM, .92 for the anterior pelvic-tilt ROM, and .87 for the total ROM. We discuss the factors that may have influenced the reliability of the measurements and the clinical implications and limitations of the test. We suggest additional research to examine intratester reliability of measuring the posterior pelvic-tilt ROM, intertester reliability of measuring all angles and ROM, and the pelvic tilt of many types of subjects.

Adult↗
PubMed1984

High-sensitivity phenylthiohydantoin amino acid analysis using conventional and microbore chromatography.

Reversed-phase microbore high-performance liquid chromatography was investigated for high-sensitivity analysis of phenylthiohydantoin (PTH) amino acids. A mixed nitrile alkylsilane bonded phase was developed and ternary gradient elution conditions were devised for resolution of the common PTH amino acids. Elution conditions were developed with a conventional 150 X 4.6 mm I.D. column and transferred to a 150 X 1 mm I.D. microbore column. The performance of these columns was evaluated in terms of PTH amino acid resolution, enhanced sample detectability, and retention time precision. For this work a general purpose high-performance liquid chromatograph was modified to reduce extra column band broadening and a preformed gradient elution technique was developed to achieve rapid analysis times at microbore flow-rates. The microbore high-performance liquid chromatographic system is useful for high-sensitivity analysis of PTH amino acids in micro-sequencing applications.

Amino Acids↗
PubMed1984

Improved method for the determination of chlorhexidine in urine.

A high-performance liquid chromatographic method is described which is suitable for the determination of the common antiseptic chlorhexidine in urine. The method employs Sep-Pak cartridges to remove chlorhexidine from the urine matrix. Chromatographic separation was achieved on a C18 reversed-phase column using a mobile phase of methanol-20 mM sodium acetate solution (60:40) adjusted to pH 5 with glacial acetic acid. An ion-pair agent (pentadecafluorooctanoic acid) was used at a concentration of 100 micrograms ml-1 X 3-Bromobenzophenone was used as chromatographic standard (kappa' = 4.0). 4-Bromobenzophenone (kappa' = 3.9) or dibenzal hydrazine (kappa' = 4.4) may also be used. A series of urine samples was analysed and no interferences were observed. The method is simple and rapid with a total analysis time of ca. 30 min.

Chemical Phenomena↗
PubMed1984

Inhibition of gluconeogenesis and glycogenolysis by 2,5-anhydro-D-mannitol.

2,5-Anhydro-D-mannitol (100 to 200 mg/kg) decreased blood glucose by 17 to 58% in fasting mice, rats, streptozotocin-diabetic mice, and genetically diabetic db/db mice. Serum lactate in rats was elevated 56% by 2,5-anhydro-D-mannitol, but this could be prevented by dichloroacetate (200 mg/kg) or thiamin (200 mg/kg). In hepatocytes from fasted rats, 1 mM 2,5-anhydro-D-mannitol inhibited gluconeogenesis from a mixture of alanine, lactate, and pyruvate. It also inhibited glucose production and stimulated lactate formation from glycerol or dihydroxyacetone. Glycogenolysis in hepatocytes from fed rats was markedly inhibited by 1 mM 2,5-anhydro-D-mannitol both in the presence or absence of 1 microM glucagon. 2,5-Anhydro-D-mannitol can be phosphorylated by fructokinase or hexokinase to the 1-phosphate and then by phosphofructokinase to the 1,6-bisphosphate. Rat liver glycogen phosphorylase was inhibited by 2,5-anhydro-D-mannitol 1-phosphate (apparent Ki = 0.66 +/- 0.09 mM) but was little affected by 2,5-anhydro-D-mannitol 1,6-bisphosphate. Rat liver phosphoglucomutase was inhibited by 2,5-anhydro-D-mannitol 1-phosphate (apparent Ki = 2.8 +/- 0.2 mM), whereas 2,5-anhydro-D-mannitol 1,6-bisphosphate served as an alternative activator (apparent K alpha = 7.0 +/- 0.5 microM). Rabbit liver pyruvate kinase was activated by 2,5-anhydro-D-mannitol 1,6-bisphosphate (apparent K alpha = 9.5 +/- 0.9 microM), whereas rabbit liver fructose 1,6-bisphosphatase was inhibited by 2,5-anhydro-D-mannitol 1,6-bisphosphate (apparent Ki = 3.6 +/- 0.3 microM). The phosphate esters of 2,5-anhydro-D-mannitol would, therefore, be expected to inhibit glycogenolysis and gluconeogenesis and stimulate glycolysis in liver.

Animals↗
PubMed1984

Possible interferon response in a child with measles encephalitis during immunosuppression.

A 4-yr-old boy with acute lymphoblastic leukaemia in remission developed an encephalopathy following an attenuated measles infection. He was treated with interferon and further deterioration was halted. Three weeks after interferon treatment was stopped, leukaemic blast cells reappeared in his peripheral blood and he died shortly thereafter. Aspects of diagnosis and treatment of this syndrome are discussed now that the presumptive clinical diagnosis is made more frequently.

Cerebral Cortex↗
PubMed1983

Phonological evaluation and remediation of speech deviations of a child with a repaired cleft palate: a case study.

Phonological evaluation of the speech of a 5-year-old boy with a repaired cleft palate identified the prevalence of the following phonological patterns: singleton obstruent omission (both prevocalic and postvocalic, but particularly prevocalic voiceless); velar deviations (omissions and fronting); cluster reduction and cluster deletion; stridency deletion (omissions and stopping); liquid deviations (omissions, gliding, stopping, nasalizing, and vowelizing); and glottal replacement. The basic aspects of an individually designed facilitative remediation program based on the subject's phonological system are presented, and the results are discussed.

Child, Preschool↗
PubMed1983

Comparison of haematological indices between women of four ethnic groups and the effect of oral contraceptives.

Six-hundred-and-eighty-two women volunteers from four ethnic groups--Black, Indian, Oriental and White took part in a study to assess differences in haematological indices between the groups. This study was part of a broad investigation into the metabolic effects of oral contraceptives. The effect of the oral contraceptive (OC) on haematological indices was analysed but not found to be significant. The haemoglobin concentrations were lower than normal laboratory reference values for White subjects in 12.5% of Indian, 16.5% of Black and 4.3% of Oriental control groups respectively. All the White control group were within the normal range. These findings were considered in relation to age, weight, pregnancies, smoking, alcohol consumption, nutritional status, and disorders of haemoglobin synthesis. A deficient iron intake accounted for the high incidence of low haemoglobin in the Indian group in whom low transferrin saturation and serum ferritin were observed. With the possible exception of inherited disorders of haemoglobin synthesis, none of the factors we considered could account for the low haemoglobins found in the Black group. Separate reference values for haematological indices in different ethnic populations are considered.

Adolescent↗
PubMed1982

Nemaline rod disease, with reference to the routine use of histochemical methods in autopsy investigations.

A 50-year-old man had suffered from an undiagnosed form of muscle disease for most of his life. Death was due to cardiac failure. Numerous samples of muscle, obtained at autopsy, were subjected to the usual histochemical techniques used in investigating neuromuscular disease. Frozen sections revealed the abnormal accumulations of Z-band protein that characterize nemaline rod disease. That this disorder would have escaped diagnosis had only routine paraffin-embedded sections been examined is emphasized.

Adenosine Triphosphatases↗
PubMed1982

Treatment of tardive dyskinesia with propranolol.

A double-blind, intensive case design was used to study the effect of propranolol on tardive dyskinesia. No short-term improvement was observed, but two of the four subjects responded to long-term propranolol use. Properties of propranolol other than acute beta blockade are suggested as possible mechanisms of action.

Adult↗
PubMed1981

Influence of dietary cholesterol and fat on serum lipids in men.

The effect of changes in dietary cholesterol and fat on serum lipids was studied in 32 healthy men (mean age = 24.8 years). Subjects were fed a controlled diet for 10 days providing 42 to 45% of the total calories from fat, a P/S ratio of 0.3 to 0.5 and two eggs per day. During the next eight weeks, 16 subjects received each of the following diets for four weeks in a crossover design: 1) a control diet with two eggs per day or 2) the control diet with eggs replaced by a cholesterol-free egg substitute. The remaining 16 subjects received each of the following diets in a similar crossover design: 1) a modified-fat diet containing 35% of the total calories from fat, a P/S ratio greater than or equal to 1.0 and two eggs per day or 2) the same modified-fat diet with the egg substitute replacing the eggs. The two-week cycle of menus repeated throughout the study included a wide variety of foods commonly consumed in this country. Although the response of individual subjects varied, analysis of variance showed a significant decrease in serum total cholesterol related to replacement of eggs with the egg substitute and to modification in the type and amount of dietary fat. A significant diet-treatment interaction or sequencing effect was not found. Change in cholesterol intake related to addition or deletion of two eggs in the daily diet had no significant effect on serum triglycerides, high density lipoprotein cholesterol, or relative lipoprotein concentrations.

Adult↗
PubMed1981

Citrus artifact interference in aflatoxin M1 determination in milk.

Dried citrus waste was fed to dairy cows, their milk was extracted, and aflatoxin M1 was quantitated by using both high pressure liquid chromatography (HPLC) and thin layer chromatography (TLC). Results indicate that a compound from the citrus waste, which is excreted into the milk, interferes with the HPLC determination of aflatoxin M1 in milk and causes a false positive test. This interference can be overcome by using TLC with proper selection of developing solvents.

Aflatoxin M1↗