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R Stefanescu

Publications and source records attributed to R Stefanescu.

5 recordsLinked to original sources

Riemannian elasticity: a statistical regularization framework for non-linear registration.

In inter-subject registration, one often lacks a good model of the transformation variability to choose the optimal regularization. Some works attempt to model the variability in a statistical way, but the re-introduction in a registration algorithm is not easy. In this paper, we interpret the elastic energy as the distance of the Green-St Venant strain tensor to the identity, which reflects the deviation of the local deformation from a rigid transformation. By changing the Euclidean metric for a more suitable Riemannian one, we define a consistent statistical framework to quantify the amount of deformation. In particular, the mean and the covariance matrix of the strain tensor can be consistently and efficiently computed from a population of non-linear transformations. These statistics are then used as parameters in a Mahalanobis distance to measure the statistical deviation from the observed variability, giving a new regularization criterion that we called the statistical Riemannian elasticity. This new criterion is able to handle anisotropic deformations and is inverse-consistent. Preliminary results show that it can be quite easily implemented in a non-rigid registration algorithms.

Algorithms↗

Epitope extraction technique using a proteolytic magnetic reactor combined with Fourier-transform ion cyclotron resonance mass spectrometry as a tool for the screening of potential vaccine lead peptides.

Epitope extraction technique is based on the specific digestion of a target protein followed by immunoaffinity isolation of a specific recognition peptide. This technique, in combination with mass spectrometry, has been efficiently used for epitope identification. The major goal of this work was to utilize newly developed enzyme and immunoaffinity magnetic reactors for the epitope extraction procedure and confirm the efficiency of this improved system for epitope screening of proteins. Alginic acid-coated magnetite microparticles with immobilized TPCK-trypsin provided high working efficiency with low non-specific adsorption, digestion time in minutes and low frequency of missed cleavages. The sensitivity and specificity of tryptic fragmentation of the beta-amyloid-peptide Abeta (1-40) as a model polypeptide was confirmed by Fourier-transform ion cyclotron resonance mass spectrometry analysis. The Sepharose reactor or immunoaffinity magnetic reactors, both with anti-amyloid-beta monoclonal antibodies, were used for specific isolation and identification of target peptides. In this way, the epitope extraction technique combined with mass spectrometric analysis is shown to be an excellent base for molecular screening of potential vaccine lead proteins.

Cyclotrons↗

A Grid service for the interactive use of a parallel non-rigid registration algorithm of medical images.

OBJECTIVE: The goal of this work is to improve the usability of a non-rigid registration software for medical images. METHOD: We have built a registration grid service in order to use the interactivity of a visualization workstation and the computing power of a cluster. On the user side, the system is composed of a graphical interface that interacts in a complex and fluid manner with the registration software running on a remote cluster. CONCLUSION: Although the transmission of images back and forth between the computer running the user interface and the cluster running the registration service adds to the total registration time, it provides a user-friendly way of using the registration software without heavy infrastructure investments in hospitals. The system exhibits good performances even if the user is connected to the grid service through a low throughput network such as a wireless network interface or ADSL.

Algorithms↗

Lasting effects on mouse brain growth of 24 hr postpartum deprivation.

When inbred BALB/c mice were separated from their mother for 24 or 36 hr beginning shortly after birth, growth of the body, whole brain and corpus callosum was almost completely stopped. After being returned to their mother, mice deprived for 24 hr gained weight more slowly than non-deprived littermates over the next 6 days but later showed moderate catch-up growth after weaning at 4 weeks of age. After 55 days of recovery, mice deprived for only 24 hr showed significant reductions in brain weight and size of forebrain commissures compared to littermate controls. Approximately twice as many deprived mice had a corpus callosum that was abnormally small compared to controls. These results demonstrate that a rather brief but severe period of separation from the mother can have lasting effects on brain growth.

Animals↗

Prenatal protein deprivation increases defects of the corpus callosum in BALB/c laboratory mice.

The development of two forebrain fiber tracts, the anterior commissure (CA) and corpus callosum (CC), was examined in BALB/cCF laboratory mice in relation to the maternal dietary protein concentration during gestation. The diets contained either 8 or 27% casein. The BALB/c strain was used because it is prone to having a corpus callosum which is either deficient in size or absent. Female mice were assigned to the low-protein diet either 2 weeks prior to mating (chronic malnutrition) or on the seventh day of gestation (acute malnutrition), and fetal brain development was assessed at 18.5 days after conception. Protein deficiency increased the number of animals which did not have a corpus callosum present in a midsagittal section. In those animals in which the structure was present, it was evident that the cross-sectional area was smaller in the chronically deprived animals than in the control group. In addition to CC area, both CA area and brain weight were reduced by protein deprivation. When brain weight was used as a covariate in an analysis of covariance the effect on CA area was no longer apparent. Part of the effect on the corpus callosum was, however, independent of the effect on brain weight and CA area.

Animals↗