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Biomedical subjects

R Stenling

Publications and source records attributed to R Stenling.

At least 55 records · Page 3Linked to original sources
PubMed1995

Vein invasion in renal cell carcinoma: impact on metastatic behavior and survival.

PURPOSE: The development of a thrombus extending into the veins is well recognized in renal cell carcinoma. We investigated the hypothesis that vein invasion alone has no adverse impact on survival but is a highly negative factor in other tumors. MATERIALS AND METHODS: In 200 consecutive patients invasion of the renal vein and vena cava was evaluated and compared with the clinical course. RESULTS: A total of 26 patients had vena caval and 47 had renal vein invasion. Patients with venous invasion had a significantly shorter survival but no survival difference was demonstrated based on the level of involvement. CONCLUSIONS: Our study indicates that vein invasion itself seems to be an important prognostic factor in renal cell carcinoma.

Adult↗
PubMed1995

Local recurrence and long-term survival in patients with gastric cancer--analysis of possible impact of clinicopathological parameters.

In a retrospective study comprising 88 patients operated upon with curative intent a number of histopathological parameters of possible prognostic value regarding local recurrence and long-time survival were analysed. Local recurrence within 5 years was found in 28 patients (32%) of which 17 (61%) were diagnosed within the first 2 years. Crude survival rates at 5 and 10 years were 25% and 15%. According to Laurén's classification the results indicated better, but not significant, 5- and 10-year survival for the diffuse type (36% vs 25%). The probability of 10-year survival suggested a better (P = 0.06) prognosis for tumours in the middle third of the stomach, and for patients operated with total gastrectomy (P = 0.025). The probability of recurrence in relation to lymph node involvement suggested a more favourable prognosis (P = 0.06) for patients without lymph node metastases, and in relation to tumour fibrosis a less favourable prognosis for pronounced fibrosis (P = 0.001).

Adult↗
PubMed1995

P53 germ line haplotypes associated with increased risk for colorectal cancer.

Three p53 DNA polymorphisms (BstU I and Msp I restriction fragment length polymorphisms (RFLPs) in exon 4 and intron 6 respectively, and a 16 bp duplication in intron 3) and their haplotype combinations were studied in patients with colorectal cancer and compared with patients with ulcerative colitis and healthy controls. There were only minor differences between patients with ulcerative colitis and controls, the only significant difference was observed in the distribution of BstU I-Msp I haplotypes. When single polymorphisms were studied, a significantly lower frequency of the 16 bp duplication was found in patients with colorectal cancer. The protective effect of the 16 bp duplication was more pronounced in haplotype combinations with the BstU I A1 and Msp I A1 alleles, whereas these alleles in combination with the 16 bp A1 allele (no duplication) were associated with an increased risk for colorectal cancer. The genotypic combination BstU I 2-1, 16 bp 1-I, Msp I 2-1 was found in 8.4% of cases among patients with colorectal cancer and 0.5% of cases in the controls (odds ratio = 18.8). The extended haplotype responsible for the high cancer risk of this genotype appears to be BstU I A1-16 bp A1-Msp I A1. The results of this study indicate that the haplotype approach to the identification of p53 germ line alleles associated with increased susceptibility to cancer is far more powerful than the analysis of single polymorphisms, since the capacity to identify germ line alleles predisposing to cancer should increase with the number of polymorphic sites included in the analysis.

Alleles↗
PubMed1995

DNA content in colorectal carcinoma: a flow cytometric study of the epithelial fraction.

The aim of this study was to estimate the proliferative activity of the epithelial fraction in human colorectal carcinomas. Cell suspensions from 27 human colorectal carcinomas were simultaneously selected for epithelial cells and analyzed for DNA content. The staining procedure we employed, after dispersing the tumour sample into a single-cell suspension, included propidium iodide and a monoclonal antibody to the intermediate filament cytokeratin specific for secretory epithelia of normal human tissue and cells of epithelial origin in adenocarcinomas. This technique made it possible to distinguish the epithelial cell population from non-epithelial cells in the tumour. Flow cytometry was used for DNA analysis and the results obtained after epithelial selection were compared with conventional DNA analysis of crude tumour tissue. No difference in S-phase values of diploid and aneuploid cytokeratin positive cells were seen, whereas analysis of crude tumour cell suspensions showed lower S-phase values in diploid tumours compared to aneuploid ones. When cytokeratin-positive cells in the tumour were selected, we found presence of diploid tumour cells in almost all aneuploid tumours.

Aged↗
PubMed1994

Colorectal cancer surveillance in patients with ulcerative colitis.

In a prospective study carried out between 1977 and 1991, 131 patients with ulcerative colitis from an area of 65,000 inhabitants were followed by clinical visits and regular colonoscopy with biopsy. At the end of the study 58 patients had had total colitis for more than 10 years, 38 had left the programme (16 after radical operations, nine because of death--one from colitis, one from carcinoma--and 13 for other reasons) and 632 colonoscopies had been performed. Colorectal carcinoma was diagnosed in four patients, of whom two were included in the programme with a diagnosis of cancer. Dysplasia was diagnosed in 24 patients, other than those with malignancy; in four this was of high grade. Carcinoma and dysplasia occurred mainly in the left colon and in patients with total colitis. The surveillance programme was resource consuming and the cost-benefit must be questioned.

Adolescent↗
PubMed1994

Cell kinetics of renal cell carcinoma studied with in vivo iododeoxyuridine incorporation and flow cytometry.

In 29 patients with renal cell carcinoma tumor cell kinetics were studied by in vivo labeling with 100 mg. iododeoxyuridine given 3 to 7 hours before nephrectomy. Two to 6 samples were analyzed from each tumor after fixation in ethanol. Iododeoxyuridine was analyzed by specific antibodies using flow cytometric analysis of labeled cells and parameters, such as labeling index and potential tumor doubling time, could be determined. Samples from 28 tumors were evaluable. Mean labeling index of the tumors was 2.7%, which was significantly different from the 0.35% index in kidney cortex tissue. There was no statistical difference in labeling index among the clinical stages. The mean potential tumor doubling time in the tumor cells was 20.3 days, which was significantly different compared with 137.7 days in kidney cortex tissue. There was a frequent intra-tumoral heterogeneity of potential tumor doubling times. Diploid and aneuploid samples had significantly different potential tumor doubling (mean 37.4 and 12.5 days, respectively). Potential tumor doubling had a significant impact on the survival time (p = 0.004), which also was noted in the patients with aneuploid tumors (p = 0.003). Our study shows that in vivo incorporation of iododeoxyuridine successfully can be analyzed in renal cell carcinoma. Potential tumor doubling time gives valuable prognostic information on the clinical behavior of the patients.

Adult↗
PubMed1994

Effects of rye bran, oat bran and soya-bean fibre on bile composition, gallstone formation, gall-bladder morphology and serum cholesterol in Syrian golden hamsters (Mesocricetus auratus).

The effects of rye bran, oat bran and soya-bean fibre on serum lipids, bile composition and gallstone formation were studied in male Syrian golden hamsters (Mesocricetus auratus). The control groups received fibre-free stone-provoking (O1 diet) or non-stone-provoking (O2 diet) diets. The serum cholesterol levels were lower for all groups fed on the diets supplemented with the dietary fibre sources compared with the control groups. The total content of bile acids in bile was higher in groups given rye-bran diets compared with the corresponding controls. The proportion of cholic acid was higher and that of chenodeoxycholic and lithocholic acid lower in the groups given rye-bran-, oat-bran- or soya-bean-fibre-supplemented diets, compared with the corresponding controls. The secondary:primary bile acid ratio was lower in the group given the rye-bran-supplemented O1 diet. The lithocholic:deoxycholic acid ratio was lower in the groups given rye-bran-, oat-bran- or soya-bean-fibre-supplemented diets than in the corresponding controls. A lower frequency of gallstones was observed only for the group receiving the rye-bran-supplemented O1 diet while the lithogenic index was lower in the groups given the rye-bran-supplemented O2 diet. A decreased epithelial volume density of the gall-bladder and an increased smooth muscular volume density were observed in animals given oat-bran- and rye-bran-supplemented O1 diets, whereas for the soya-bean-fibre-supplemented O1 diet, only the smooth muscular volume density was increased.

Animals↗
PubMed1994

Effect of rye bran on excretion of bile acids, cholesterol, nitrogen, and fat in human subjects with ileostomies.

The excretion of bile acids, cholesterol, dry matter, nitrogen, fat, and energy in ileostomy effluent, and plasma lipid concentrations were studied in eight subjects with ileostomies. The subjects consumed a wheat bread-based, low-fiber diet (LFD) for 3 wk and a rye bran bread-based, high-fiber diet (HFD) for 3 wk. The ileal excretion of dry matter, nitrogen, fat, and energy was higher during the HFD period. The daily excretion and the percentage of conjugated bile acids were significantly higher and the percentage of free bile acids lower in the ileostomy effluents during the HFD as compared with the LFD period. No significant difference in the excretion of cholesterol, net cholesterol, sterol, or net sterol was noted between the HFD and LFD periods. No significant differences in plasma concentrations of HDL-, LDL-, and total cholesterol, and apolipoprotein A-I and B were observed between the two 3-wk dietary periods.

Adult↗
PubMed1994

Application of dual parameter analysis in flow cytometric DNA measurements of paraffin-embedded samples.

In a comparison of flow cytometric DNA measurements on fresh and paraffin-embedded material from primary squamous cell carcinomas of the head and neck region, we discovered that previously undetected aneuploid clones could be detected by dual parameter analysis of cytokeratin and DNA applied to disintegrated cells from paraffin sections. Using this new approach the correlation coefficient between DNA-indices from fresh and paraffin-embedded material increased from 0.423 to 0.904.

Aneuploidy↗
PubMed1994

Impact of familial amyloid associated polyneuropathy on duodenal endocrine cells.

Duodenal endocrine cells in 11 patients with familial amyloid associated polyneuropathy (FAP) were compared with those in 12 healthy volunteers by means of immunohistochemistry and morphometry. The total endocrine cell content, determined by the argyrophilic reaction and chromogranin A immunoreactivity, was significantly reduced in FAP patients compared with controls. There was a significant reduction in the serotonin, cholecystokinin/gastrin, and secretin immunoreactive cell content. A decreased cell content was also noted for somatostatin and gastric inhibitory polypeptide immunoreactive cells but this was not statistically significant. Amyloid deposits were noted in seven of the 11 biopsy specimens from FAP patients, but otherwise the duodenum was histologically normal in both groups. The reduction in endocrine cell content was not correlated with the degree of amyloid deposit in the duodenum. These findings indicate that patients with FAP have reduced intestinal endocrine cells. This does not seem to be related to amyloid deposits in the mucosa or to villous or crypt abnormalities. The observed changes in endocrine cells may contribute to the development of intestinal motility dysfunction and maldigestion in these patients.

Adult↗
PubMed1994

Proliferation of human renal cell carcinoma studied with in vivo iododeoxyuridine labelling and immunohistochemistry.

Tumor cell proliferation is one interesting cell biological parameter of possible predictive value for prognosis. In the present study 176 tumor and 33 kidney cortex samples from 33 patients with renal cell carcinoma were studied after immunohistochemical detection of in vivo labelled iododeoxyuridine. The tumors had a mean labelling index (LI) of 1.06 +/- 0.59%, significantly different from LI of kidney cortex tissue 0.08 +/- 0.09% (p < 0.001). There was a tendency towards increasing LIs at higher clinical stages. No correlation was found between LI and tumor size. A significant difference existed for LIs between diploid and aneuploid tumors (p = 0.037). Within the limited number of patients with a relatively short follow-up, Kaplan-Meier curves indicated that LI might provide prognostic information.

Adult↗
PubMed1994

Cell kinetics of head and neck squamous cell carcinomas. Prognostic implications.

Forty-three squamous cell carcinomas of the head and neck region were in vivo labelled with the thymidine analogue iododeoxyuridine. Combined flow cytometric (FCM) and immunohistochemical (IHC) analysis was performed, and the following parameters calculated: labelling index (LI), S-phase time (TS) and potential tumor doubling time (Tpot). Complete FCM and IHC analyses could successfully be performed in 31 cases, showing a median LI of 13.6% with FCM and 9.1% with IHC. A correlation achieved between LI/FCM and LI/IHC was due to the aneuploid cases, whereas the diploid cases showed no such correlation. Data indicated that Tpot calculated with LI from IHC (Tpot/IHC) might be a prognostic factor, in contrast to Tpot determined using LI/FCM.

Adult↗
PubMed1993

Tumor-cell proliferation and prognosis in renal-cell carcinoma.

In the present study the prognostic value of tumor S-phase fraction was evaluated in 69 patients with renal-cell carcinoma. The proportion of S-phase cells was calculated from DNA histograms by flow cytometry in multiple samples from each tumor. The mean tumor S-phase percentage varied between 1.0 and 17.0%, mean 7.5%, with a significant difference between diploid and non-diploid tumors. Stage-I tumors showed significantly lower S-phase values than tumors of stages II/III and IV. Histopathological grade correlated with fraction of cells in S-phase. Nineteen tumors were homogeneously diploid, I was tetraploid and 49 were aneuploid. Heterogeneity concerning S-phase values was found in 46 of the 69 tumors and concerning DNA ploidy in 34 tumors. Survival time was longer for patients with diploid tumors than for those with aneuploid tumors. Patients with S-phase values < 7.5% had a longer survival time than patients having tumors with S-phase values > 7.5%. Within the group of aneuploid tumors, patients with S-phase values < 7.5% had a significantly better prognosis. In multivariate analysis, only tumor stage and S-phase gave significant independent prognostic information. The S-phase fraction seems to be an additional prognostic parameter for patients with renal-cell carcinoma.

Adult↗
PubMed1993

DNA fingerprinting of renal cell carcinoma with special reference to tumor heterogeneity.

Genomic alterations in renal cell carcinoma were investigated by DNA fingerprinting using the simple repetitive oligonucleotide probe (CAC)s. Nine of ten tumors showed somatic changes in the fingerprint pattern compared with constitutional DNA. The most consistent changes were deletions and/or decrease in intensity of a band. When using two or three samples from different parts within the tumor, up to three different cell clones could be detected. These results indicate that DNA fingerprinting analysis can be a useful technique for the study of genomic alterations and tumor heterogeneity in renal cell carcinoma.

Carcinoma, Renal Cell↗
PubMed1993

Effects of oat and rye fractions on biliary and faecal bile acid profiles in Syrian golden hamsters (Mesocricetus auratus).

The effects of bran and starchy endosperm fractions of oat and rye on faecal weight and on biliary and faecal bile acids were studied in Syrian golden hamsters (Mesocricetus auratus). The animals fed on diets supplemented with steam-flaked oat bran, oat bran or rye bran had higher wet and dry weights of faeces compared with the animals fed on the fibre-free or low-fibre endosperm diets. A higher mean percentage of biliary cholic acid and a lower mean percentage of chenodeoxycholic and lithocholic (LCA) acids was observed in the bran-supplemented dietary groups. Animals fed on the bran-supplemented diets had increased daily faecal excretion of both total saponifiable and total free bile acids compared with the animals fed on fibre-free or endosperm-supplemented diets. The mean percentage of total saponifiable bile acids in the faeces was higher, and that of free bile acids lower in the animals fed on bran-supplemented diets. A significantly lower concentration of faecal free LCA was observed in the animals fed on the rye-bran diet. Both bran and endosperm diets reduced the faecal LCA:deoxycholic acid compared with the fibre-free diet, but the bran diets had a more pronounced effect than endosperm diets.

Animals↗
PubMed1993

Oat bran, rye bran, and soybean hull increase goblet cell volume density in the small intestine of the golden hamster. A histochemical and stereologic light-microscopic study.

Small-intestinal morphology, particularly the amount of goblet cells, was studied in Syrian golden hamsters fed either a low-fibre (0.5% fibre) diet or the same diet supplemented with oat bran, rye bran, or soybean hull (12% fibre) during 6 weeks. All fibre diets increased the density of goblet cell volume to epithelial volume and to villus volume in the ileum and to some extent in the jejunum and also the number of goblet cells related to surface traces of villi both in the proximal and distal small intestine. The weight of the small intestine and the circumference and the volume of the intestinal wall in the ileum were greater in the rye bran diet group as compared with all the other groups, suggesting hyperplasia. These results suggest that oat bran, rye bran, and soybean hull supplementation might result in higher goblet cell secretory activity in both the proximal and distal small intestine of hamsters. The various fibre sources influenced the small-intestinal wall differently.

Animals↗
PubMed1993

Peptidergic innervation and endocrine cells in the human liver.

Histologically normal liver biopsy specimens from patients with Hodgkin's lymphoma were investigated with three immunohistochemical methods for the occurrence of peptidergic nerve fibers and endocrine cells. Numerous immunoreactive nerve fibers were seen with antisera against peripheral nerves markers (neuron-specific enolase, neurofilament protein, and S-100). These nerve fibers were localized in the tunica media of branches of both the hepatic artery and portal vein, around the bile ducts, and in the connective tissue of the interlobular septa. In the liver, 10 types of peptidergic nerve fibers were detected: glucagon-, glucagon-like peptide- (GLP), somatostatin-, neuropeptide Y- (NPY), vasoactive intestinal polypeptide-, neurotensin-, gastrin/cholecystokinin C-terminus-, substance P-, serotonin-, and galanin-immunoreactive nerve fibers. GLP-, somatostatin-, NPY-, neurotensin-, substance P-, and galanin-immunoreactive nerve fibers were abundant; the other nerve fibers were scarce. The nerve fibers showed two distinct patterns of distribution: they occurred in the blood vessel wall and in connective tissue of the interlobular septum. Pancreatic polypeptide- and NPY-immunoreactive cells were found among the lining epithelial cells of the bile ducts in the interlobular septum.

Adult↗