Biomedical subjects

R Stowers

Publications and source records attributed to R Stowers.

3 recordsLinked to original sources

PubMed1996

Ability of spleen, peritoneal cavity, and lymph node B cells to reconstitute serum immunoglobulin in SCID mice.

The impact of intrinsic B lymphocyte heterogeneity and of microenvironmental influences on serum immunoglobulin production by B cells was examined by intravenous (i.v.) and intraperitoneal (i.p.) transfer of BALB/c and BALB.xid (X-chromosome-linked immunedefective; XID) lymph node (LN), splenic (SP) and peritoneal cavity (PerC) cells into severe-combined immune-defective (SCID) mice. The results indicate that each B-cell source restores all immunoglobulin classes within 5 weeks of transfer, the rates for each isotype, however, differ between the B-cell sources. Serum IgM levels were restored most rapidly by PerC cell transfer, followed by SP and LN cell transfer. In addition, normal immunoglobulin levels were reached in the absence of complete lymphoid reconstitution. Serum immunoglobulin phenotypes characteristic of the donor strain, e.g. reduced IgM and IgG3 production by XID B cells, were maintained after transfer into the SCID recipient. Microenvironmental influences were indicated by reduced immunoglobulin production after i.p. transfer and after i.v. transfer into irradiated SCID recipients. The data show that both B-cell type and microenvironment play significant roles in generating the heterogeneous pool of B cells required for humoral immunity.

Animals↗

PubMed1993

Laparoscopically harvested omental free flap to cover a large soft tissue defect.

OBJECTIVE: The omentum has been a very important tool in the armamentarium of the reconstructive surgeon. It has lost much of its value because of the morbidity associated with laparotomy. Laparoscopic surgery has become a popular technique and allows operations to be performed with minimal morbidity. The possibility of harvesting the omental free flap with the laparoscope and its use in reconstructive surgery has been demonstrated. SUMMARY BACKGROUND DATA: Since the first laparoscopic cholecystectomy was performed, many surgeons have learned the procedure. Other surgical specialties have also benefited from this technique. The omentum provides a large amount of vascularized tissue and excellent wound coverage. It can be transferred as a pedicle flap, or as a free flap, using microvascular technique. METHODS: The procedure was developed and refined in an animal model. One team harvested the omentum with laparoscopic assistance, while the other team prepared the recipient vessels. After completion of the microvascular transfer, the dogs were observed for 14 days. At that time, the omental tissue was examined for gross and histologic changes. A clinical case is also presented. RESULTS: Gross and microscopic studies documented the viability of this approach. The patient tolerated the procedure well and had an unremarkable postoperative course. CONCLUSIONS: Experimental and clinical evidence shows that the omentum can be successfully harvested as a free flap using laparoscopic assistance. This technique may prove to be of clinical significance and very useful for reconstructive surgery with less morbidity.

Animals↗

PubMed1990

Regional cooperative summer internship program for pharmacy students.

A regional summer internship program for pharmacy students that was coordinated by seven hospitals is described. Recruiting for the Mid-Missouri Summer Intern Program began during the winter of 1987-88. Students were recruited from pharmacy schools throughout the Midwest and were provided with housing and financial compensation while in the program. Each participating hospital agreed to hire at least one intern and appointed a preceptor or coordinating pharmacist, who met with the intern regularly. Students got most of their training and work experience at their home hospital; however, they also rotated to the other participating hospital pharmacy departments. Students had to keep a daily diary of their activities and to complete individual special projects. At the end of the summer, students and preceptors completed a written evaluation of the program. Students indicated that the program provided a valuable and unique educational experience. Preceptors thought that fourth-year and fifth-year students were best suited for the program. The hospital pharmacy directors agreed that the opportunity to evaluate a student's work ethic and professional capabilities before offering a position was a benefit. A regional summer internship program was successful in introducing pharmacy students to institutional pharmacy practice.

Curriculum↗