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Biomedical subjects

R Sugimoto

Publications and source records attributed to R Sugimoto.

At least 37 records · Page 2Linked to original sources
PubMed1999

Hakata antigen, a new member of the ficolin/opsonin p35 family, is a novel human lectin secreted into bronchus/alveolus and bile.

Hakata antigen was first reported as a serum protein that reacted with an autoantibody from patients with systemic lupus erythematosus. Recently, it has been found that Hakata antigen is a new member of the ficolin/opsonin p35 family, which is a distinct lectin family, on the basis of homology of structures and the common characteristic of possessing lectin activity. In this study we analyzed the tissue distribution of Hakata antigen. Hakata antigen mRNA and protein were generated in the lung and liver. In the lung, Hakata antigen was produced by both ciliated bronchial epithelial cells and Type II alveolar epithelial cells and was secreted into the bronchus and alveolus. In the liver, Hakata antigen was produced by bile duct epithelial cells and hepatocytes and was also secreted into the bile duct. These results demonstrate that Hakata antigen is a unique lectin protein that exists not only in serum but also in bronchus/alveolus and bile, and indicate that Hakata antigen plays a role in bronchus/alveolus and bile under physiological conditions.

Antibodies, Monoclonal↗
PubMed1999

Angiomyolipoma of the liver--a case report and review of 48 cases reported in Japan.

Hepatic angiomyolipoma was considered to be a rare benign tumor, but the number of cases has been increasing recently as imaging techniques improve. We describe a case of hepatic angiomyolipoma for which a definitive diagnosis could not be made on imagings and in which resection was performed. The patient had anti-HCV antibody and slight dysfunction of the liver. The tumor showed a heterogeneous high echo on ultrasonography and a low attenuation value of +32.6 Housfield Units, which was much higher than fat density, on plain computed tomography. Discrimination from hepatocellular carcinoma with fatty change was difficult preoperatively. Microscopically, the tumor consisted of spindle-shaped and epithelioid smooth muscles, adipose tissues and proliferating blood vessels and these histological findings confirmed the diagnosis of hepatic angiomyolipoma. The appearance of hepatic angiomyolipoma on imaging diagnosis varies widely due to the fact that the relative proportion of vessels, muscles and fats varies widely from tumor to tumor. The tumor in our case had relatively few fat components. We review 48 cases reported in Japan and discuss imaging diagnosis and surgical indications for tumors.

Angiomyolipoma↗
PubMed1998

Cloning and characterization of the Hakata antigen, a member of the ficolin/opsonin p35 lectin family.

The Hakata antigen is a novel, thermolabile beta2-macroglycoprotein that reacts with sera from patients suffering from systemic lupus erythematosus. In this study we present the structure and the function of the Hakata antigen. We have identified cDNA clones encoding the Hakata antigen and analyzed its function. The cDNA included a possible open reading frame of 897 nucleotides, encoding 299 amino acids. The Hakata antigen consisted of a collagen-like domain in the middle section and a fibrinogen-like domain in the COOH terminus, both of which are homologous to human ficolin-1 and opsonin P35, indicating that these three molecules form a distinct family. The molecular mass of the Hakata antigen expressed in transfected cells was 35 kDa under reduced conditions, and it formed ladder bands under nonreducing conditions compatible with the previous result that the Hakata antigen exists in serum as homopolymers. Purified Hakata antigen sustained lectin activity, showing affinity with GalNAc, GlcNAc, D-fucose as mono/oligosaccharide, and lipopolysaccharides from Salmonella typhimurium and Salmonella minnesota. These results suggest that the Hakata antigen, a new member of the ficolin/opsonin P35 family, plays a role in the serum exerting lectin activity under physiological conditions.

Amino Acid Sequence↗
PubMed1996

Investigation on neurotoxicity of occupational exposure to cyclohexane: a neurophysiological study.

OBJECTIVES: To examine the effect of occupational exposure to cyclohexane on the peripheral nervous system. METHODS: A nerve conduction study was performed on 18 workers exposed to cyclohexane in a luggage factory and on age and sex matched occupationally unexposed controls. 12 workers had been exposed to n-hexane (median 2.8 years) before the start of exposure to cyclohexane. To confirm the effect of exposure, a follow up study was performed on nine workers one year after the first study. The mean exposure to cyclohexane was 1.2 years in the first study. A symptom survey was performed. The exposure was measured by air sampling of the breathing zone of each worker. The urinary metabolite cyclohexanol was also monitored. RESULTS: The concentration of airborne cyclohexane ranged from 5 to 211 ppm. The urinary concentration of cyclohexanol ranged from 0.12 to 1.51 mg/l. There was a strong correlation between the cyclohexane exposure in personal air and urinary cyclohexanol. No differences were found in nerve conduction velocities (NCV) between workers exposed to cyclohexane and age and sex matched controls. The results of the follow up study showed significant improvements in peroneal motor NCV (P < 0.01) and sural sensory NCV (P < 0.05) and in ulnar motor distal latency (MDL, P < 0.05) and peroneal MDL (P < 0.05) compared with the first study. Although the past n-hexane exposure affected the first neurophysiological study, the effect had disappeared in the second study, one year later. CONCLUSION: Occupational exposure to the concentrations of cyclohexane experienced in this study had no adverse effects on the peripheral nervous system.

Adult↗
PubMed1994

Exposure monitoring and health effect studies of workers occupationally exposed to cyclohexane vapor.

A survey was conducted in the second half of a working week on 33 women who either applied glue (with cyclohexane as an almost exclusive solvent component) or worked in the vicinity of glue application. Carbon cloth-equipped diffusive samplers were used for personal measurement of time-weighted average intensity of exposure to the solvent. The geometric mean and the highest cyclohexane concentration observed in air were 27 ppm and 274 ppm, respectively. Concentrations of cyclohexanol in urine samples and cyclohexane in whole blood and serum collected at the end of a shift showed significant correlations with the solvent exposure levels. Urinary cyclohexanone also correlated, but with a smaller correlation coefficient. The observation suggests that cyclohexanol in urine and cyclohexane in blood or serum collected at the end of a shift are useful indicators of occupational exposure to cyclohexane vapor. Quantitative estimation of balance at the end of the shift suggested that only a minute portion (< 1%) of cyclohexane absorbed is excreted in the urine as cyclohexanol, almost exclusively as a glucuronide. A survey of subjective symptoms revealed an increase in the prevalence of "dimmed vision " and "unusual smell", but hematology and serum biochemistry testing did not indicate any specific signs.

Adult↗
PubMed1989

Allergic contact dermatitis from croconazole hydrochloride.

We report 6 cases of contact sensitivity to croconazole hydrochloride, a new imidazole antimycotic drug introduced to the Japanese market in 1986, and available as 1% gel and cream. 6 sensitized patients reacted on patch testing to croconazole hydrochloride down to 0.5 to 0.1% pet, and 3 appeared to be cross-sensitized to sulconazole nitrate. In Japan, allergic contact dermatitis to this drug has now been detected in 12 cases, including our own 6. Prescribers should be aware of contact sensitivity to this drug.

Administration, Cutaneous↗
PubMed1989

[Bone and suppurative lesion distribution of antimicrobial agents (ampicillin and ofloxacin) in experimental infected rabbits].

Using Streptococcus milleri and Bacteroides fragilis in the mandibula of the domestic rabbit, a jaw-bone infected model was prepared; ampicillin (ABPC) a penicillin derivative and ofloxacin (OFLX) a pyridonecarboxylic acid derivative, were administered orally, injected intramuscularly, or infused intravenously; their concentration in the pus, mandibula, supramaxilla, humerus, femur, sternum, ilium and serum were measured. Pharmacokinetic studies compared the penetration. (1) By oral administration, the maximum ABPC concentration in the pus and bone tissues was 5.9 to 34.3% of the serum, by intramuscular injection 10.4 to 27.5% and by i.v. infusion 12.1 to 42.4%. (2) The maximum concentration of orally administered OFLX in the pus and bone tissues was 52.5 to 111.7% of the serum, by intramuscular injection 56.4 to 100.3% and by i.v. infusion 61.2 to 121.8%. (3) When administered orally, injected intramuscularly, or infused intravenously ABPC showed good penetration into the pus, mandibula and ilium, and OFLX into the pus, sternum and ilium. When infused intravenously, OFLX in the pus and bone tissues was higher than the MIC of both strains. (4) When ABPC was administered as under 3 routes, T1/2 was longer in the pus and most bone tissues than in the serum. T1/2 of OFLX was shorter in most bone tissues by all administration routes than in serum, T1/2 was longer for OFLX than for ABPC, except when orally administered. (5) AUC of ABPC was in the order of intravenous infusion, intramuscular injection and oral administration. About the same AUC of OFLX was shown after i.v. infusion and i.m. injection, both values being about double after oral administration. In all administration routes, AUC of pus surpassed AUC of serum and AUC of OFLX greatly surpassed that of ABPC.

Ampicillin↗