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Biomedical subjects

R T Judson

Publications and source records attributed to R T Judson.

9 recordsLinked to original sources
PubMed1993

Variable rejection patterns of cultured keratinocyte allografts in the rabbit.

There are many conflicting reports on the survival of cultured keratinocyte allografts (CKAs). Studies were performed in the rabbit model to elucidate further the fate of CKAs. Of 24 CKAs, 7 were identified as technical failures, 13 displayed classical macroscopic evidence of rejection with a prolonged mean survival time of 2.8 days, compared to non-cultured allograft controls (p < 0.01), and 4 failed to display typical macroscopic evidence of rejection. Furthermore, the time to eventual wound healing of the classically rejected CKAs were delayed by 6.0 days (p < 0.0001), compared to identical non-grafted control wounds.

Animals↗
PubMed1990

In vitro culture of disaggregated rabbit keratinocytes.

A technique is described for the culture of disaggregated rabbit keratinocytes and the production of confluent sheets of immunologically pure keratinocytes suitable for use in transplantation studies. The benefits of various feeder layers, nutrients and growth hormones were examined by the use of paired controls. The ability of the cultured keratinocyte sheets to be removed from the culture vessel intact and survive as viable autografts was demonstrated, thus establishing a new animal model for the investigation of allograft rejection responses to cultured keratinocytes. The benefits of using the rabbit model for such studies and the advantages of this culture technique over a previously described method of rabbit keratinocyte cell culture are discussed.

Animals↗
PubMed1989

Review of keratinocyte culture techniques: problems of growing skin.

Keratinocyte cell culture has been studied by a large number of investigators, resulting in an extensive body of often contradictory literature. The advantages and disadvantages of the two major techniques, explant and disaggregated culture, will be examined, followed by a critical evaluation of the major technical variables. Suggested standardized methods for both explant and disaggregated culture, and the indications for the use of each, will be proposed. Finally, further directions for basic research will be suggested in order to improve this useful tool.

Cells, Cultured↗
PubMed1987

Blood transfusion and recurrence of cancer of the colon and rectum.

Clinical and experimental studies indicate that transfusion of allogeneic blood has immunomodulating properties, and that the behaviour of some tumours may be influenced by the immune system of the host. Eighty-seven patients who had undergone 'curative' resection of cancer of the colon or rectum were studied retrospectively to determine whether blood transfusion adversely affected the rate of cancer recurrence. Fifty-three patients (61 per cent) were transfused and 34 were not. The transfused and non-transfused groups were comparable in terms of age, sex, duration of follow-up, mode of presentation, stage of disease and haemoglobin level at discharge from hospital, and differed in admission haemoglobin and distribution of tumour locations (right- or left-sided colonic or rectal tumours). Recurrent malignancy was detected in 36 per cent of transfused patients and 26 per cent of non-transfused patients (P greater than 0.1). There was no association between the number of transfusions and recurrence for any tumour site or stage. The incidence of recurrence was significantly higher in those patients who received transfusions during surgery than in those who received transfusion either before or after surgery (chi 2 = 7.01, d.f. = 1, P less than 0.01) or no transfusions (chi 2 = 4.23, d.f. = 1, P less than 0.05). The study indicates that factors influencing the need for blood transfusion during operation had a greater bearing on prognosis than receipt of a blood transfusion per se and that future prospective studies investigating the association between transfusion and cancer recurrence need to determine accurately the indications for transfusion.

Aged↗
PubMed1985

Blood transfusion and tumour growth: an experimental study.

An animal model using RIII mice, which have a high incidence of spontaneously occurring mammary tumours, was used to study the effects of blood transfusion on tumour growth. Virgin female mice were transfused with various dilutions of blood from C57b1 mice either before or both before and after inoculation of tumour cells from a tumour line. Allogeneic transfusion prior to inoculation did not affect survival compared with syngeneic transfusion. Both allogeneic and syngeneic blood given before and after tumour inoculation led to a prolonged survival compared with saline infusion. Survival was longest following syngeneic blood. The effect was attributed to the general beneficial effects of blood transfusion and not the result of immunological changes.

Animals↗
PubMed1984

Wound infection following renal transplantation.

A retrospective survey of wound infection following renal transplantation in 100 consecutive patients after the introduction of pre-operative antibiotic prophylaxis is reported. There was one wound haematoma but no wound infection in the 100 primary transplant wounds, and one haematoma which was followed by secondary infection in the 23 patients in whom transplant nephrectomy was performed. There were no other major wound complications. It is concluded that careful surgical technique and antibiotic prophylaxis can virtually eliminate the potentially grave complication of wound infection in this high risk group of patients.

Abscess↗
PubMed1983

Torrential rectal bleeding. An unusual presentation of colonic carcinoma.

A case of torrential rectal bleeding due to a colonic carcinoma is reported. The clinical diagnosis was confirmed by angiography, which showed an abnormal circulatory pattern at the hepatic flexure of the colon. Resection of the tumour controlled the bleeding. Dealing with this clinical problem requires a systematized approach. Angiographic demonstration of the source of the bleeding allows definitive surgical management.

Adenocarcinoma↗