[Hormonal regulation of the activity of some ornithine-cycle enzymes].
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Biomedical subjects
R Tarnawski
Publications and source records attributed to R Tarnawski.
[Early effect of hydrocortisone and prednisolone administration on the activity of aspartate transcarbamylase (E. C. 2. 1. 3. 2.) in rat liver].
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Origin of serum alpha-amylase isoenzymes from healthy adult rats.
In connection with common use of rats in experimental testing drug toxicity in the liver or pancreas, we checked the electrophoretic patterns of amylase isoenzymes extracted from the liver, pancreas, parotis and serum of healthy adult rats. Separation of isoenzymes was made by polyacrylamide gel electrophoresis. The results indicate the salivary glands and liver as sources of serum alpha-amylase.
Animals↗
Selective augmentation of radiation effects by 5-fluorocytosine on murine B16(F10) melanoma cells transfected with cytosine deaminase gene.
We report that in vitro 5-fluorocytosine sensitizes B16(F10) melanoma cells to radiation damage when they are transfected with cytosine deaminase gene (CD). The greatest enhancement of radiation cytotoxicity was observed when B16(F10)/CD cells were incubated in medium with 500 microM 5-fluorocytosine for 3 hours, with incubation starting 1 hour after irradiation. 5-Fluorocytosine did not change radiosensitivity of parental, nontransfected cells. The isoeffective dose for CD-transfected cells treated with 5-fluorocytosine was reduced by 20% at a 2-Gy level of effect for nontransfected cells. We believe that the observed outcome is related to 5-fluorouracil generated by CD and subsequent 5-fluorouracil anabolites. Our results support the development of in vivo models for tumor radiosensitization using the CD gene/5-fluorocytosine system.
Animals↗