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Biomedical subjects

R Tilly

Publications and source records attributed to R Tilly.

28 records · Page 2Linked to original sources
PubMed1977

Some properties of cells cultured from early-lactation human milk.

Cells that can be cultured from pools of early-lactation milk were studied. Under the culture conditions used, the majority of cells attached to collagen-coated dishes; most of these remained single, did not divide, and in their adhesiveness, phagocytic ability, and ultrastructure resembled macrophages or histiocytes. On a plate seeded with approximately 3 X 10(5) cells, however, 10-100 colonies of dividing cells developed. These cells had the junctional complexes typical of epithelial cells and grew well in a medium supplemented with human serum and hydrocortisone for 16-20 days after seeding. After removal of serum from the medium, some cells continued to traverse the cell cycle, and the colonies containing these cells were morphologically distinct from those which became quiescent. The nondividing cells in milk could be separated from the milk epithelial cells and were able to stimulate the growth of epithelial cultures from benign mammary dysplasias.

Breast Neoplasms↗
PubMed1976

Transmission of auto-immune haemolytic anaemia and murine leukaemia virus in NZB-BALB/c hybrid mice.

When mated to normal BALB/c partners, male and female NZB mice transmitted auto-immune haemolytic anaemia to three generations of their hybrid progeny. Red cell auto-antibodies (positive Coombs tests) were detected, on average, 11 months later in the F1 hybrids than in the parental strain, and the course of the disease was protracted. In explicably, the auto-immune reactions then appeared progressively earlier in successive generations of both croses. The Coombs reactions of the F1 and F2 hybrids were often weak and inconsistent, while those of F3 offspring showed the strong and stable picture typical of NZB mice. The incidence of auto-immune disease in each generation, although similar in the reciprocal crosses, indicated that the pattern of inheritance was very complex. The hybrids did not develop the lymphoid type B reticulum cell neoplasia which characterizes auto-immune NZB mice. Instead, and irrespective of Coombs status, they had lymphocytic leukaemias, lung adenomas, hepatomas and type A reticulum cell neoplasms of the liver. Murine leukaemia virus was identified electronmicroscopically in F1 embryos, and in all the adults examined. It was also isolated from leukaemic spleen cells passaged briefly in vivo, and from malignant hepatic (reticulum) cells maintained in vitro. These isolated were leukaemogenic in newborn BALB/c, NZB, and F1 hybrid recipients, but did not induce or accelerate positive Coombs reactions. Only a small proportion of the hybrids had significant glomerulonephritis, and overt kidney disease was minimal. The lesions were not confined to Coombs-positive mice. Possible links between auto-immunity, malignancy, and virus infection in NZB mice are discussed in the light of these results.

Anemia, Hemolytic, Autoimmune↗