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Biomedical subjects

R Tirri

Publications and source records attributed to R Tirri.

At least 19 recordsLinked to original sources
PubMed2000

The predictive value of attributes of pain to classify rheumatoid arthritis.

Out of 2300 patients with rheumatic diseases 1627 were analysed to develop a classification of rheumatoid arthritis based on clinical attributes of pain. Of these, 641 patients had the disease and 986 were controls with other rheumatic conditions. For traditional format classification, six of eight variables were selected: pain at a fixed joint; symmetrical pain; continuous pain; pain mainly present at night or in the morning; pain following joint pressure; and pain decreased by load/movement. The occurrence of four or more of these features was associated with a 72.1% sensitivity and a 79.1% specificity. A classification tree constructed on four features that showed the greatest diagnostic power (symmetrical pain, pain mainly present at night or in the morning, pain at joint pressure, continuous pain), was associated with a 75.8% sensitivity and a 77.0% specificity.

Adult↗
PubMed2000

Pott's disease of the lower cervical spine in a diabetic patient.

Over the last few years, tuberculosis has steadily been returning to the worlds attention as a major health threat. The spinal localization of tuberculosis (Pott's Disease) represents around 1% of the case histories, in 3-5% of which there is cervical involvement. Diabetes mellitus increases the risk of infectious disease and predisposes to tuberculosis. Here, a rare case is presented of Pott's disease in the lower cervical spine, associated with retropharyngeal abscess in a diabetic patient. The aim of this study is to emphasize the importance of early diagnosis in such patients, so that adequate pharmacological and/or surgical treatment can be initiated to avoid serious complications.

Cervical Vertebrae↗
PubMed1994

[The nephrotic syndrome and pheochromocytoma. A report of a rare clinical case].

In clinical practice, the coincidence of nephrotic syndrome with pheochromocytoma is very rare. The case is described of a 23-year-old woman who in June 1988 presented with recurrent hypertensive crises, severe asthenia, abundant sweats, orthostatic hypertension and massive proteinuria. Diagnostic tests performed (abdominal ultrasound and CT, urinalysis, renal function tests, plasma levels of metanephrine and normetanephrine, as well as urinary VMA determination) revealed the presence of a pheochromocytoma of the left adrenal gland combined with nephrotic syndrome. Surgical removal of the left adrenal led to immediate normalization of blood pressure and absence of urinary abnormalities. The authors therefore suggest either an immunological pathogenesis or one due to glomerular hyperfiltration.

Acute Disease↗
PubMed1993

Cardiac involvement in rheumatoid arthritis: an echocardiographic study.

Thirty-nine consecutive patients with rheumatoid arthritis (RA) and 40 control subjects were studied by echocardiography in order to assess the incidence of cardiac involvement in this disease. The occurrence of anatomic lesions in our series was lower than that observed in other studies. No differences in mean values of left and right ventricular diastolic function indexes obtained by Doppler echocardiography were found between patients and controls. However, in 26% of patients with RA, left ventricular abnormalities probably secondary to myocardial fibrosis were observed.

Arthritis, Rheumatoid↗
PubMed1991

Evidence for peripheral impaired glucose handling in patients with connective tissue diseases.

Sixteen patients suffering from rheumatoid arthritis (RA) (n = 8), systemic lupus erythematosus (SLE) (n = 5), and systemic sclerosis (SSc) (n = 3), and 10 healthy subjects matched for age, sex, and body mass index, were submitted to an intravenous (IV) glucose tolerance test (GTT) (0.33 g/kg of body weight in 3 minutes) and to a euglycemic hyperinsulinemic glucose clamp to study insulin response and action. In the euglycemic clamp, along with the two insulin infusion rates (0.5 mU/kg.min from 0 to 120 minutes and 1 mU/kg.min from 121 to 240 minutes), a primed (20 microCi) continuous (0.2 microCi/min) infusion of 3H-glucose allowed determination of glucose kinetics. Our data show that patients versus controls have (1) a significant increase in basal plasma insulin levels (87.2 +/- 14.8 v 41.3 +/- 6.0 pmol/L, P less than .05); (2) similar glucose-induced acute insulin response; and (3) a lower glucose disappearance rate (Rd), glucose metabolic clearance rate (gMCR), and glucose infusion rate (GIR) when the lowest insulin infusion rate was delivered. These differences disappeared when the insulin infusion rate was doubled. Furthermore, basal plasma insulin levels and glucose disappearance rate significantly correlated with the main inflammatory indices of each disease studied. We conclude that in our patients impaired glucose handling is mainly due to peripheral insulin resistance.

Arthritis, Rheumatoid↗
PubMed1991

Antinuclear antibodies in first-degree relatives of patients with polymyositis-dermatomyositis: analysis of the relationship with HLA haplotypes.

Antinuclear antibodies (ANA), as detected by indirect immunofluorescence on HEp-2 cells, have been investigated in five spouses and 41 first-degree relatives of nine probands with polymyositis-dermatomyositis (PM-DM) and in 41 sex- and age-matched controls. ANA were detected in 12 out of the 41 first-degree relatives and in two controls (chi 2 = 6.97; P less than 0.01). HLA typing was done in four out of the nine families; in two of them only, ANA segregated with a haplotype. ANA positivity was not correlated either to sex or to age or to household contact. Our results show that ANA occur in a significant percentage of first-degree relatives of patients with PM-DM. The finding seems to be genetically conditioned.

Adolescent↗
PubMed1987

Isolation and characterization of single myocardial cells from the quail, Coturnix coturnix japonica.

1. The enzymatic cell isolation technique was applied to the bird heart resulting in myocytes of which 10-50% maintained their spindle-shaped morphology, excluded the vital dye, Evans blue and tolerated physiological concentration of Ca2+ ions. 2. The length of spindle-shaped myocytes was on average 289 +/- 7 microns, and the maximum width was 10.2 +/- 0.3 microns. The mean length of the sarcomeres was 2.18 +/- 0.03 microns. 3. In electron micrographs the fine structure of the spindle-shaped myocytes looked normal--regular sarcomeric organization with clear A and I bands, mitochondria with tightly located cristae and well-developed sarcoplasmic reticulum (SR). 4. Most (80%) of the spindle-shaped myocytes were quiescent in physiological calcium concentration and practically all of them could be induced to twitch by electric field stimulation. Some beat spontaneously showing mostly slowly-propagating (135 +/- 6 microns/sec at 20 degrees C) contraction waves, so-called phasic contractions. Sometimes spontaneous twitch-type contractions could also be seen.

Animals↗
PubMed1986

Isolation and characterization of single myocardial cells from the perch, Perca fluviatilis.

In applying the enzymatic cell isolation technique to the fish heart about 40% of the dispersed myocytes maintained their spindle-shaped morphology, and about half of them tolerated physiological concentration of Ca2+ and excluded the vital dye, Evans blue. The length of spindle-shaped myocytes was on average 133 +/- 3 micron and the maximum width was 4.2 +/- 0.1 micron. The mean length of the sarcomeres was 2.1 +/- 0.1 micron. The sizes of the myocytes did not vary significantly with the weights of the fish. Electron microscopic examinations showed typical fish myocardial cell structure; absence of transverse tubule system, a sparse network of sarcoplasmic reticulum and from a few up to eight or more myofibrils. The cells were mononuclear. Most of the Ca2+-tolerant myocytes were quiescent, but the contraction in them could be induced by electric field stimulation. Both the spontaneous and electrically triggered contractions were of twitch type. The slowly propagating contraction waves, so-called phasic contractions common in isolated mammalian cardiac myocytes, could not be seen at all.

Animals↗
PubMed1984

Alpha and beta adrenergic control of contraction force of perch heart (Perca fluviatilis) in vitro.

Noradrenaline showed a negative inotropic effect on the isolated electrically triggered atrium of the perch. The effect was stronger at lower temperature and was antagonized by an alpha adrenergic blocker, phentolamine. The inotropic effect of adrenaline was dependent on incubation temperature. The effect was negative at 15 C but biphasic at 24 C, where with increasing adrenaline concentration a positive inotropic effect was followed by negative inotropy. Phentolamine not only antagonized the negative inotropic effect of adrenaline at 15 degrees C but changed it to positive. This positive inotropic effect was antagonized by a beta adrenergic blocker, propranolol. On the triggered ventricular strip adrenaline had no effect at 6 or 15 C, but increased contraction force at 24 C. It can be suggested that in the perch heart atrium there is an activity balance of alpha and beta receptors, which mediate the negative and positive inotropic control, respectively. As in higher vertebrates, alpha adrenergic activation decreases and beta activation increases by agonists in the following order: noradrenaline, adrenaline and isoprenaline. The balance changes towards increased beta activity when temperature rises.

Animals↗
PubMed1983

Membrane potential oscillations in enzymatically isolated rat myocardial cells.

Enzymatical isolation was used for preparation of single myocardial cells, contractions of which were mainly of slow phasic type but normal fast contractions also occurred. Membrane potential (MP) changes of these cells were measured by conventional microelectrode techniques. Initial MP level of these cells varied between -60 to -90 mV (74.4 +/- 1.9). In the majority of the cells the MP rapidly dropped to -20 to -40 mV and continued to decline while oscillating. In some cells MP recovered from -20 to -40 back to a more negative potential, and the MP oscillation was strongest (up to 20 mV) between -30 to -50 mV. In about 3% of the impalements the MPs did not degenerate at all, but stayed at initial values for several minutes. The MP oscillations were dependent on the MP level and connected to the slow phasic contractions. In connection to the normal fast contractions, two types of action potentials (AP) could be registered. Near -50 mV the cells often generated slow APs of duration 200-400 ms and dV/dt less than 3 V/s but at higher MP level normal fast APs up to 120 mV (100-300 ms and dV/dt greater than 40 V/s) were generated. The slow phasic contractions were never connected to either type of AP.

Action Potentials↗
PubMed1983

Effects of acetylcholine, isoprenaline, phenylephrine and dibutyryl-cAMP on the contractility of isolated myocytes of the rat heart ventricles.

Single ventricular myocytes were isolated by enzymatic dissociation from the rat heart. The myocytes showed two types of spontaneous contractions: action potential dependent 'electric' contractions which occurred as short bursts, and slowly propagating contraction waves, i.e. phasic contractions. Electric contractions were normally found only in some of the myocytes, but in about 85% of the cells these could be induced by isoprenaline (ISO), phenylephrine (PHE), dibutyryl-cAMP (DB-cAMP) or acetylcholine (ACh). The inducing potency of the drugs was ACh greater than PHE greater than ISO greater than DB-cAMP. When electric contractions were blocked with verapamil, the frequency of phasic contractions was increased by ISO and DB-cAMP, whereas PHE and ACh had not this effect. In 45 min, ISO increased the frequency tenfold. High concentrations (10(-4) and 10(-5) M) of ISO lead to steady contracture of the myocytes in about 40% of the cells. The effect of DB-cAMP was weaker being only about 20% of that of ISO and it never caused steady contractures. The results suggest that alpha receptor activation by PHE does not affect the intracellular calcium movements but that ISO through beta receptor activation and DB-cAMP directly may increase the rate of calcium accumulation by sarcoplasmic reticulum (SR).

Acetylcholine↗