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Biomedical subjects

R Tobe

Publications and source records attributed to R Tobe.

18 recordsLinked to original sources
PubMed2000

Promotive effects of a silk film on epidermal recovery from full-thickness skin wounds.

We examined the effects of the transparent fibroin film (silk film) on full-thickness skin wounds. Full-thickness dermatotomies (15 mm x 9 mm) were prepared on the dorsal wall of CRJ:CD-1 nu/nu (ICR nu/nu) mice. The area of the wounds dressed with silk film was reduced to 10% of that made by the dermatotomy 14 days after the dermatotomy and were covered with regenerated epidermis 21 days after the dermatotomy. In contrast, less recovery and epidermal regeneration were found 14 days after dermatotomy in the wounds dressed with a conventional hydrocolloid dressing (Duro Active). Furthermore, only partial incomplete epidemal growth was obtained 21 days after dermatotomy. Most importantly, the healing time of wounds dressed with silk film was 7 days shorter than those dressed with DuoActive dressing. The silk film showed an almost similar or slightly better promotive effect as the lyophilized porcine dermis (Alloask D), which is used as a dressing for burns, ulcers, and decubitis. Histologic findings revealed that there was greater collagen regeneration and less inflammation and neutrophil-lymphocyte infiltration of the wounds dressed with silk film than with DuoActive dressing. It is clear that regeneration of the epidermis and dermis of the wound beds covered with silk film was faster than with DuoActive dressing. Finally, silk film is easily obtainable, sterilizable, and transparent, and it allows easy observation of tissue recovery. Therefore, silk film offers advantages over other dressings and may be clinically useful for wound treatment.

Animals↗
PubMed1988

Significance of 2-3 and 2-6 sialylation of Lewis a antigen in pancreas cancer.

Distributions of sialylated derivatives of Lewis a (Lea) antigen in cancer tissue of the human pancreas and in the sera of patients with pancreas diseases have been studied; the significance of 2-3 and 2-6 sialylation of Lea antigens in pancreas cancer have been investigated using specific monoclonal antibodies. In most pancreas cancer tissue the 2-3 sialylated Lea antigen was found to be specifically distributed in cancer cells as determined by immunohistologic techniques, while a significantly smaller amount of the antigen was detected in surrounding nonmalignant pancreas tissue, which was infiltrated by cancer cells. Conversely, the 2-6 sialylated Lea antigen was abundantly present in nonmalignant pancreas tissue, while it was less frequently present in pancreas cancer cells. When the sera of 66 patients with pancreas diseases were examined for these sialylated Lea antigens, correlation studies showed that the levels of 2-3 sialylated Lea tended to be 44.1 times more than the levels of 2-6 sialylated Lea in the sera of cancer patients. The average ratio of 2-3 sialylated Lea to 2-6 sialylated Lea was 0.23 in the sera of patients with pancreatitis. These data collectively indicate that the 2-3 sialylation of Lea is remarkably enhanced, and the 2-6 sialylation of Lea antigen is suppressed in pancreas cancer. The determination of the 2-3 sialylated Lea to 2-6 sialylated Lea ratio in patients with pancreas diseases may be helpful for the differential diagnosis of pancreas cancer and nonmalignant pancreatic disorders.

Antibodies, Monoclonal↗
PubMed1988

Semiquantitative in vitro binding assay of indium-111-labeled monoclonal antibodies to human cancer and normal tissues.

We have developed a simple in vitro method for the semiquantitative assessment of the radiolabeled antibody binding to cancer and normal tissues. Indium-111-labeled F(ab')2 fragments of 17-1A and 19-9 monoclonal antibodies with well-characterized specificity for gastrointestinal cancer demonstrated similar binding properties between cultured cancer cells and membrane fractions of homogenates prepared from tumor tissues. All of the 17 colon cancer specimens and seven (64%) of 11 gastric cancer specimens obtained by surgery showed positive binding with 17-1A. Specific binding of 19-9 was observed in 9 (53%) colon cancers and 4 (36%) gastric cancers. However, some normal colon tissues were also positive with 111In-labeled 17-1A. Relative levels of CA 19-9 antigen expression, determined by the binding with radiolabeled antibodies, correlated with percent positive cells determined by the immunohistochemical assays. Furthermore, membrane fractions could be cryopreserved without losing antibody-binding activity. These results indicate that this assay can be used for testing the immunoreactivity of radiolabeled anti-tumor antibodies and in vitro binding properties to cancer and normal tissues.

Antibodies, Monoclonal↗
PubMed1987

[Clinical study of the NCC-ST-439 EIA kit using serum from patients with various types of cancer and benign disease (1)].

The amounts of antigens in the serum of patients with various types of cancer and benign diseases were measured in order to clinically evaluate the NCC-ST-439 EIA kit. From the results of measurements in 583 healthy persons, cut-off values of 4.5 U/ml for males and females 50 years or older and 7.0 U/ml for females 49 or younger were set. The false-positive rate for healthy persons at this time was 5.0%. The positive rates for malignant tumors at these cut-off values were 58.9% for pancreatic carcinoma, 53.1% for cholangiocarcinoma, 41.9% for mammary carcinoma, 36.6% for rectal and colonic carcinoma, 33.3% for hepatocellular carcinoma and 35.3% for carcinoma as a whole. The false-positive rates for benign diseases were 14.0% for pancreatic disease, 9.7% for liver disease, 8.0% for biliary duct disease and 8.3% for benign diseases as a whole. These rates were low when compared with those for other tumor markers. These results showed that NCC-ST-439 is a tumor marker with a high degree of specificity.

Adolescent↗
PubMed1987

[Clinical study of the NCC-ST-439 EIA kit using serum from patients with various types of cancer and benign disease (2)].

The positive rates for NCC-ST-439 in cases where other tumor markers were negative were 16.8% for CA19-9-negative cancer cases and 23.5% for CEA-negative cancer cases. The positive rates obtained using a combination of these three markers were 86% for pancreatic and cholangiocarcinomas, and at least 65% for hepatocellular, rectal and colonic and mammary carcinomas. The following combinations of tumor markers were the best from the standpoint of diagnostic efficiency: NCC-ST-439 + CA19-9 for pancreatic carcinoma, NCC-ST-439 + AFP for hepatocellular carcinoma, and NCC-ST-439 + CEA for rectal and colonic carcinoma. In cases which were positive preoperatively, 83.3% became negative after curative surgery, which reflected the clinical course very well. These results indicate that NCC-ST-439 improves the diagnostic rate in combination with other tumor markers because of its high specificity, and it was evaluated as useful for the monitoring of therapeutic effects.

Adolescent↗
PubMed1983

"Masked" Ph1 chromosome in a complex three-way translocation.

A patient with myelofibrosis was found to have a 46,XX,del(1)(q24),del(11)(p11),-22,+mar karyotype in unstimulated peripheral blood (PB) and spleen cells. On detailed cytogenetic examination it was determined that this patient had an apparently "masked" Ph1 chromosome contained in a complex three-way translocation. Since phytohemagglutinin (PHA)-stimulated PB and spleen cells were essentially normal, the masked Ph1 chromosome was assumed to be an acquired cytogenetic abnormality. The portion missing from the masked Ph1 chromosome was apparently translocated onto del(1). Thus, the detailed karyotype was 46,XX,t(1;11;22)(q24;p11;q11 or q12),t(1;22)(q24;q11 or q12). This complex rearrangement was present primarily in cells belonging to the granulocyte-macrophage cell lineage, whereas E-rosetting cells, and presumably T lymphocytes, had normal karyotypes.

Aged↗
PubMed1980

Carcinoma arising in a choledochocele.

A case of carcinoma arising in a choledochocele (type III of biliary cyst in Alonso-Lej classification) in a 61-year-old woman is presented. This is the first case reported of a malignant change developing in a choledochocele.

Adenocarcinoma, Papillary↗
PubMed1979

Legionnaires' disease in a prepaid medical-care group in Seattle 1963--75.

The community incidence of legionnaires' disease (L.D.) was estimated by a retrospective study of stored paired sera from 500 patients treated for pneumonia in Seattle in 1963--75. The patients were all members of a prepaid medical-care group; 84% were treated as outpatients. Only 5 (1%) had a fourfold rise in antibody titre to the L.D. antigen. Seroconversion to influenza A virus also occurred in 3 of the 5 cases. The course of illness in 4 of the 5 patients with confirmed infection was milder than that previously reported with legionnaires' disease. In addition, 1 child had a fourfold fall in titre, and 23 patients had titres of greater than or equal to 256 without significant change in titre. Only 8 of the 23 had their first serum collected later than 2 weeks after onset of illness, which suggests that the majority had acquired their antibodies before their attack of pneumonia. Prevalence of antibody in the 500 patients was not affected by age, sex, or year in which illness occurred. The incidence of legionnaires' disease based on 5 patients with rises in titre was estimated to be 0.4--2.8/10 000 persons/year.

Adolescent↗
PubMed1978

Stimulatory effect of adenosine on hepatic adenine nucleotide and energy charge levels in shocked rats.

In hemorrhagic shocked rats, the hepatic energy charge (ATP+1/2ADP)/(ATP+ADP+AMP) fell rapidly from 0.857 to 0.542 within 2 hours and was rapidly restored to normal levels after completing the reinfusion of shed blood. The total adenine nucleotide levels decreased slowly from 3.83 to 2.57 mumoles per g wet liver 2 hours after shock, and increased to normal levels 2 hours after reinfusion of shed blood. The intraperitoneal injection of 2 mg adenosine/100g body weight, 85 mg glucose/100 g body weight and 3.3 units insulin/100 g body weight, simultaneously with the reinfusion of shed blood, enhanced the recovery of energy charge and adenine nucleotide levels. The injection of 2 mg adenosine alone was ineffective in restoring the energy charge. However, administration of more than 6.7 mg adenosine/100 g body weight enhanced recovery of energy charge and adenine nucleotide levels, whether insulin was added or not.

Adenine Nucleotides↗