Culturing cells in a mechanically active environment.
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Animals↗
Biomedical subjects
R Tran Son Tay
Publications and source records attributed to R Tran Son Tay.
Osteoblasts increase their rate of division and align in response to cyclic, mechanical tension in vitro.
Bone adapts to physical deformation in vivo, yet the mechanism of the adaptive process remains unknown. One reason for this perplexity has been the difficulty in examining the effects of a well-defined deformation regimen on individual bone cells. With the utilization of novel, flexible-bottomed cell culture plates, one can study the effects of cyclic strain on the morphologic and biochemical adaptations of individual osteoblasts in vitro. Avian, calvarial osteoblast-like cells, from passes 2-5, responded to cyclic strain, by increasing their rates of DNA synthesis and cell division during the first 72 h after initiation of a continuous deformation regimen comprised of 3 cycles per min of 0-24% elongation. In addition, within hours after initiation of the deformation regimen, cells oriented 90 degrees to the applied strain field at the periphery of the culture plate in the region of maximum strain and elongation.
Animals↗
Mathematical model of the velocity field external to a tank-treading red cell.
The velocity field external to a stationary ellipsoidal particle with continuously rotating surface motion driven by a surrounding shear flow is calculated. The configuration is intended to model the so-called "tank-treading" behavior of mammalian erythrocytes (red cells) when suspended in shear flow. The boundary-value problem posed is based on the model developed by Keller & Skalak (7) and is solved by adapting Jeffrey's general solution (9) for the Stokes flow about a rigid, freely rotating ellipsoid immersed in an unbounded viscous flow. Streamlines and velocity profiles in the plane of symmetry are obtained by numerical computations. The flow pattern reveals two free stagnation points near the ends of the particle and the streamlines branching from these points delineate a region of closed streamlines surrounding the particle and two recirculating wakes extending to infinity both upstream and downstream of the particle. The presence of the wakes suggests a mechanism for enhanced diffusion of smaller solute particles in the surrounding fluid.
Blood Flow Velocity↗