The conditions were analyzed for evaluation of a mean soil biodegradation of a pesticide within a certain region. Different soils from two Czech and two German regions were used for a highly sensitive analysis based on a laboratory incubation of each isolated active soil strain with a radiolabeled pesticide. It was proved for all analyzed soils that the biodegradation activity of the total biomass was caused by only a small part of the present microbial strains. Not only bacteria, but also fungi and yeast have to be taken into consideration in a pesticide biodegradation. The biodegradation products were quantified by radio high-performance liquid chromatography.

Animals↗

PubMedDate not supplied

Degradation of a radiolabeled juvenile hormone analog using two insect species.

A synthetic insect juvenile hormone analog (a juvenoid), ethyl N-[2-[4-[[2,2-(ethylenedioxy)cyclohexyl]methyl]phenox]ethyl]carbam ate, which has displayed high biological activity against different insect species and high stability under field conditions, was selected as a biologically active model compound for a study of a juvenile hormone analog degradation. The biologically active compound itself and its three diversely radiolabeled derivatives were applied to the flesh fly (Sarcophaga bullata) or the tsetse fly (Glossina palpalis), respectively. Monitoring of a fate of the applied juvenile hormone analog was carried out using a detection method of the radioactivity microdistribution within the whole insect body in combination with a radio high performance liquid chromatography (radio-HPLC), both of whole-body extracts made in different, but in advance scheduled, time intervals, and of extracts of insect excreta accumulated over an eight-day experiment.

Animals↗