Clinical governance. Shift workers.
Explore the source record for details and available documents.
Chief Executive Officers, Hospital↗
Biomedical subjects
R Tyndall
Publications and source records attributed to R Tyndall.
Methotrexate-anticollagen conjugate inhibits in vitro lens cell outgrowth.
After-cataract, or posterior lens capsule opacification, is an undesirable but common sequela to extracapsular cataract surgery. We are investigating biochemical means to prevent after-cataract formation, which can be applied at the time of the original surgery. Based on similar research efforts in cancer chemotherapy, we have prepared a conjugate of the antimetabolic agent methotrexate with an antibody specific for basement membrane collagen, the major protein in the lens capsule. The conjugate was evaluated using biochemical measurements, and retained both antimetabolic and antibody activities. When the conjugate was applied to bovine posterior capsules in vitro, or in vivo in rabbits, it was an effective inhibitor of lens epithelial cell outgrowth in cell culture.
Animals↗
Quantation by flow microfluorometry of total cellular DNA in Acanthamoeba.
The DNA content of five species of Acanthamoeba was determined by flow microfluorometry. Acanthamoeba castellanii (AC-30), acanthamoeba polyphaga (APG and P-23), acanthamoeba rhysodes, acanthamoeba culbertsoni (A-1), and acanthamoeba royreba were grown in a casitone based medium 24-48 HR. The trophozoites were harvested, and evaluated for DNA-bound fluorescence. All species tested has DNA values between 2.0-5.0 pg/cell. These results placed DNA/cell values of Acanthamoeba slightly lower than DNA/cell values of other eucaryotic cells and much lower than Amoeba proteus values. These results indicate that FMF may be a useful adjunct in distinguishing Acanthamoeba cells from either eucaryotic cells or some other amoeba. However, differences in DNA/cell between species of Acanthamoeba are small and would not be useful in identification of species.
Amoeba↗