Biomedical subjects

R Uibo

Publications and source records attributed to R Uibo.

62 records · Page 4Linked to original sources

PubMed1984

The relationship of parietal cell, gastrin cell, and thyroid autoantibodies to the state of the gastric mucosa in a population sample.

The occurrence of tissue autoantibodies in relationship to fundal and antral gastritis was examined in an Estonian rural population sample, consisting of 227 persons 15 to 69 years of age. The state of the gastric mucosa was verified with multiple biopsy specimens obtained at direct-vision gastroscopy. Isolated fundal gastritis was characterized as the presence of parietal cell (PCA) and/or thyroid antibodies. PCA was seen only in persons with normal antral mucosa. On the other hand, gastrin cell antibodies (GCA) showed no correlation to the extent of antral gastritis, being seen in normal antrum or in superficial or slight atrophic gastritis but not in moderate or severe gastritis. The findings of our present study thus strengthen the view that the occurrence of PCA is mainly limited to isolated fundal gastritis and excludes the occurrence of antral gastritis. On the other hand, an association of GCA and antral gastritis could not be established.

Adolescent↗

PubMed1981

Immunology of chronic gastritis.

65 patients with chronic gastritis and 12 controls with normal gastric mucosa were studied for cell-mediated immunity by the means of the lymphocyte blasttransformation and leukocyte migration inhibition tests. In these patients parietal cell antibodies were determined by the indirect immunofluorescence test as well. In 22 patients who had eigher isolated fundal gastritis, isolated antral gastritis or pangastritis cellular immune reactions to fundal or/and antral mucosal antigen extracts were revealed, whereas only 8 had parietal cell antibodies in their sera. It is suggested that for immunological characterization of chronic gastritis the evaluation of cellular immune reactions must be undertaken as well as antibody determinations.

Adolescent↗

PubMed1981

Gastric ulcer, gastritis and gastric carcinoma.

The results of an endoscopic study of the incidence of gastritis and gastric cancer in gastric ulcer in a series of 112 patients 1 to 4 years (mean 2.6 years) after the initial examination are presented. Chronic antral gastritis was found in 48 % of cases and was mainly atrophic. In 10 cases both the antral and body mucosa were normal. In 11 cases the antral mucosa was normal before treatment and remained normal in most of them. During the observation period 5 patients died from other than gastrointestinal diseases. Eleven patients were operated on for complications or relapses of ulcer disease. Gastric cancer was found in 4 cases, the interval between the diagnoses of benign ulcer and cancer was from 1 to 27 months. Elevation of the blood carcinoembryonic antigen (CEA) level was found in 17 cases out of 56 examined and these values remained elevated in most cases at re-examination 3 years later.

Adult↗

PubMed1976

Leukocyte migration inhibition and parietal cell antibody test in chronic gastritis without pernicious anaemia.

Forty-one patients with chronic gastritis and 12 healthy persons with normal gastritic mucosa were examined. Cell-mediated immunity against gastric fundal and antral mucosal antigens was studied by means of the leukocyte migration inhibition agarose test (LMAT), and serum parietal cell antibodies (PCA) were examined by the immunofluorescence method. The LMAT was positive in 8 out of 41 patients with gastritis and in none of the healthy subjects. In all these cases migration inhibition appeared with antigen that corresponded to gastric lesion. PCA were present in 11 out of 41 patients with gastritis and in 2 out of 12 healthy persons. No significant relationship was found between the PCA test and the result of LMAT.

Adult↗

PubMedDate not supplied

The immunopathogenesis of primary biliary cirrhosis.

There have been several recent advances in our understanding of primary biliary cirrhosis. Foremost amongst these has been the cloning and identification of the mitochondrial autoantigens as members of the 2-oxo-dehydrogenase complex. These include the E2 components of PBC, BCKD and OGDC. The immunodominant autoepitopes of the autoantigens have been mapped and, in all cases, correspond to the inner lipoyl domain. Limited progress has also been made on T cells, particularly the CD4 response. However, the fundamental mechanisms and the role, if any, of CD8 cells are unknown. Finally, at least 2 groups have identified a molecule that cross-reacts with PDC-E2, i.e., a mimic, on the luminal surface of biliary epithelium in PBC but not controls. The identification of this molecule will be critical in further understanding the immune response of this disease.

Autoantibodies↗

PubMedDate not supplied

The epidemiology of primary biliary cirrhosis: immunological problems.

Primary biliary cirrhosis (PBC) has received considerable attention as an autoimmune disease, where in addition to immunological mechanisms, environmental factors may have an important role. Therefore the epidemiological analysis of disease prevalence and progression in association with immunological studies has been one of the hot areas of PBC research during recent years. In this paper the epidemiological data on PBC and its immunological marker--anti-mitochondrial antibodies--have been presented from a comparative viewpoint.

Autoantibodies↗

PubMedDate not supplied

Clinical significance of different antinuclear antibodies patterns in the course of primary biliary cirrhosis.

BACKGROUND/AIMS: The significance of antinuclear antibodies (ANA) in primary biliary cirrhosis (PBC) patient is still controversial in the literature. The purpose of this paper is to investigate the clinical significance of ANA in PBC patients. MATERIALS AND METHODS: Sixty-nine patients with PBC were investigated. Control groups included 21 patients with autoimmune hepatitis, 26 patients with alcoholic liver disease, 13 patients with systemic connective tissue disease and 27 healthy persons. ANA was detected by an immunofluorescence method on rat liver tissue sections and HEp-2 cells at serum dilution 1/40. RESULTS: In 48 out of 69 PBC patients (70%), ANA was positive in HEp-2 cell line, but in rat liver tissue sections only 29% of patients had positive ANA reactions. Most frequent patterns were multiple nuclear dots (MND) in 42% and perinuclear in 16%. MND-ANA was also found in two autoimmune hepatitis patients and in one systemic lupus erythematosus patient. Survival from the moment of developing first symptom(s) attributable to liver disease was longer in the ANA positive patients than ANA negative ones (p < 0.02). Despite immunosuppressive treatment, in most of ANA positive patients (73%) ANA did not disappear. Most frequent ANA patterns in autoimmune hepatitis and systemic connective tissue diseases patients were homogeneous and anticentromere, respectively. CONCLUSIONS: Immunofluorescence method on HEp-2 cell line for ANA detection is more sensitive than on rat liver tissue sections. In PBC patient's incidence of ANA, especially MND-ANA is a frequent immunological abnormality. ANA positive patients have better prognosis for survival.

Analysis of Variance↗

PubMedDate not supplied

Antineutrophil cytoplasmic antibodies in Estonian patients with inflammatory bowel disease. Prevalence and diagnostic role.

BACKGROUND/AIMS: In Estonia, the incidence of ulcerative colitis and especially Crohn's disease appears to be rare. Antineutrophil cytoplasmic antibodies (ANCA) are frequently found in ulcerative colitis but less frequently in Crohn's disease, their pathophysiological significance is still unclear. METHODOLOGY: Fifty-nine serum samples from patients with ulcerative colitis, 17 with Crohn's disease, 25 with irritable bowel syndrome, and 86 healthy persons were studied. Sera were analyzed for the presence of ANCA by indirect immunofluorescence, and enzyme-linked immunosorbent assay for specific ANCA using different antigens was performed. RESULTS: ANCA were detected in 29 of 59 (49%) patients with ulcerative colitis, 4 of 17 (24%) patients with Crohn's disease, and in 4 of 111 (4%) controls. The immunofluorescence staining was mostly perinuclear (pANCA). There was no correlation between ANCA and the duration or extent of the inflammatory bowel disease. In specific enzyme-linked immunosorbent assays, only 14 sera elicited binding above the normal range. CONCLUSIONS: Although the prevalence of ulcerative colitis and Crohn's disease in Estonia is much lower than in European countries, there seem to be no differences in the presence of ANCA.

Adult↗