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Biomedical subjects

R Utiger

Publications and source records attributed to R Utiger.

5 recordsLinked to original sources
PubMed1977

Juvenile hyperthyroidism with elevated thyrotropin (TSH) and normal 24 hour FSH, LH, GH and prolactin secretory patterns.

An 11 year old boy was found to have hyperthyroidism and elevated serum TSH concentrations. Hyperthyroidism was first diagnosed at 4 years of age. After antithyroid drug therapy, a subtotal thyroidectomy was done when he was 7 years old. Goiter and hyperthyroidism gradually recurred, and an elevated serum TSH concentration (90 microU/ml) was found when first measured at age 12 years. There was no evidence of a pituitary tumor. Thyrotropin-releasing hormone (TRH) administration resulted in a marked increase in serum TSH concentrations. Triiodothyronine, thyroxine and dexamethasone administration lowered the serum TSH concentration. There was diurnal variation in TSH secretion. Growth hormone (GH) and prolactin responses to provocative stimuli and 24 h secretion patterns were normal. FSH and LH secretion was normal for age and stage of sexual development. The 24 h cortisol pattern demonstrated normal episodic secretion, although the mean 24 h concentration was high (10.5 microgram/dl). These data suggest that this patient's TSH hypersecretion is due to partial resistance of the thyrotrophs to the inhibitory action of thyroid hormone.

Adolescent↗
PubMed1969

Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate.

A sensitive and specific radioimmunoassay for adenosine 3',5'-cyclic phosphate (cyclic AMP) has been developed which allows measurement of the nucleotide in extracts of 5-10 mg of tissue. The radioimmunoassay is sufficiently specific for cyclic AMP to eliminate the need for prior chromatographic separation of the cyclic nucleotide from other tissue nucleotides. The radioimmunoassay system is based upon competition of cyclic AMP with a labeled cyclic AMP derivative of high specific activity for binding sites on an antibody specific for the cyclic nucleotide. Antibody to cyclic AMP was obtained by immunizing rabbits with an antigen prepared by conjugating succinyl cyclic AMP with human serum albumin. A high specific activity derivative of cyclic AMP was prepared by synthesizing succinyl cyclic AMP tyrosine methyl ester (SCAMP-TME) and iodinating the phenolic hydroxyl group of the tyrosine moiety with (125)I. Free and antibody-bound (125)I-SCAMP-TME were separated by precipitation of the antibody-bound fraction with a second antibody (goat anti-rabbit gamma globulin). Displacement of (125)I-SCAMP-TME by unlabeled cyclic AMP when plotted as a semilogarithmic function was linear over a concentration range of 2-100 picomoles. The specificity of the antibody was tested against structurally related nucleotides, nucleosides, and purine bases. All had less than 0.005 per cent of the potency of cyclic AMP in inhibiting (125)I-SCAMP-TME binding. The marked differences in affinity of the various cyclic nucleotides to cyclic AMP antibody would suggest that antibodies can be developed for each of the cyclic nucleotides by the principles used in this work.

Adenine Nucleotides↗