Characterization of the light-driven sodium pump of Halobacterium halobium. Consequences of sodium efflux as the primary light-driven event.
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Biological Transport, Active↗
Biomedical subjects
R V Greene
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Proton movements in response to a light-driven electrogenic pump for sodium ions in Halobacterium halobium membranes.
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Cell Membrane↗
Sodium-stimulated glutamate uptake in membrane vesicles of Escherichia coli: the role of ion gradients.
Membrane vesicles prepared from Escherichia coli B/r grown on glutamate as a sole source of carbon and energy require sodium for glutamate accumulation when energized by D-lactate oxidation. Glutamate uptake can also be driven by a prearranged sodium gradient (out to in) in the absence of an energy source or a protonmotive force. Sodium ions are exchanged rapidly in respiring vesicles and the sodium gradient may be large enough under certain conditions to drive glutamate uptake after the protonmotive force is abolished with m-chlorocarbonylcyanide phenylhydrazone. Glutamate uptake due to a prearranged sodium gradient or lactate oxidation is inhibited by monensin but not by nigericin. Transport does not occur in response to valinomycin-induced membrane potential. We interpret these results to indicate that glutamate transport is obligately coupled to sodium transport and can only occur when there is a net flux of sodium ions. This flux is driven by a chemical gradient of sodium that is created by the protonmotive force generated by respiration.
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