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Periodicals as Topic↗
Biomedical subjects
R V Harrison
Publications and source records attributed to R V Harrison.
Motion sickness. Helping patients tolerate the ups and downs.
The sensory conflict theory provides a useful framework for understanding motion sickness. Few modifications of this model have been proposed since its publication. The weak link in the study of motion sickness has been the failure to delineate the anatomic structures that correspond to the model's components. Effective pharmacotherapy is available for treatment of motion sickness. The choice should be based on the need to provide prophylaxis for or treatment of symptoms and on the side effect profile of the drug.
Adaptation, Physiological↗
Reorganization of auditory cortex after neonatal high frequency cochlear hearing loss.
Cochleotopic representation in cortex (AI) is extensively reorganized in cats having neonatal, bilateral high frequency cochlear hearing loss. Anterior areas of AI, normally devoted to high frequencies, contain neurons which are almost all tuned to one lower frequency. This frequency corresponds, at the level of the cochlea, to the border between normal and damaged haircell regions.
Amikacin↗
Developing criteria for ordering common ancillary services.
This article describes the process utilized to develop criteria for appropriately ordering five ancillary services frequently performed in hospitals: 1) arterial blood gases, 2) electrocardiograms (ECGs), 3) serum electrolytes, 4) chest x-rays, and 5) complete blood counts (CBCs). The development of each set of criteria involved an initial consultant, a panel of six additional regional expert consultants, and three national reviewers. Each criteria set was developed through a process involving seven steps: 1) an initial working draft, 2) revisions at an initial meeting of the regional experts, 3) revisions at a second meeting of the regional experts, 4) written comments from the regional experts, 5) written comments from national reviewers, 6) additional written comments from regional experts, and 7) application of the criteria to cases in a community hospital. The change in item content was measured between steps and agreement with individual items was measured at steps 2, 3, and 4. The results indicate that appreciable change in content occurred with each step except step 4. Agreement started fairly high and was over 90% by step 4. The discussion considers the utility of each developmental step, factors affecting the utility of the criteria, the extent to which the results can be generalized, and the need for more research to identify optimal processes for the development of criteria to evaluate quality of care.
Ancillary Services, Hospital↗
Morphological changes to the cochlea in an animal model of profound deafness.
We have used systemic application of the ototoxic drug amikacin, to induce total cochlear haircell loss in the chinchilla, in order to create an animal model of profound deafness. We have produced models of both neonatal deafness (by treatment of new-born pups), as well as of acquired total hearing loss (by treatment of mature animals). We present a description of our techniques for producing cochlear lesions and for evoked potential monitoring of the resulting threshold elevations. We also give a qualitative description of cochlear haircell damage as viewed by scanning electron microscopy, and a quantitative assessment of the spiral ganglion cell survival after total haircell loss.
Amikacin↗
Cytoarchitecture of cochlear nucleus in the chinchilla.
The morphology of the cochlear nucleus in the normal, adult chinchilla, as demonstrated by Nissl staining, was examined. The cytoarchitecture was determined from sections viewed at the light microscope level. The chinchilla cochlear nucleus was found to possess most of the features reported in other mammalian cochlear nuclei. It could easily be divided into dorsal and ventral components due to an intervening layer of granule cells, and most cell types previously reported in mammals were also found in the chinchilla cochlear nucleus. A distinct distribution pattern of cell types exists within each part.
Animals↗
Effects of total cochlear haircell loss on integrity of cochlear nucleus. A quantitative study.
In cochleas of chincillas treated with amikacin, cochlear sensory cells were totally destroyed in all new-born animals. In animals treated as adults some occasional haircells remained in apical turns. In the neonatally treated animals, the resulting auditory deprivation significantly affected the volume of the ventral cochlear nucleus and large-dark spherical cell area. The density of large-dark spherical cells increased significantly from normal in both neonatally and adult treated groups. Our results suggest that the VCN is more dependent on auditory stimulation for proper development than the DCN. In adult chinchillas treated with amikacin there was a significant change in large-dark spherical cell density without a change in total cell numbers or large-dark spherical cell area volume. Our study indicates that the mature cochlear nucleus is much more resistant to the effects of auditory deprivation than the developing cochlear nucleus and that the maintenance of the mature auditory system is not as dependent on auditory stimulation. Studies such as this examining the morphological effects of profound cochlear deafness on higher levels of the auditory system are essential in cochlear implant research.
Amikacin↗
The effects of long and short term profound deafness on the responses of inferior colliculus to electrical stimulation of the cochlea.
It is widely assumed that adequate sensory stimulation is important for the proper post-natal development of the central nervous system, particularly during certain critical or sensitive periods in development. To examine the functional effects of long-term profound deafness on the development of the auditory system, the response properties of neurons in the inferior colliculus (IC) to electrical stimulation of the cochlea were investigated in chinchillas (i) deaf from birth and (ii) deafened as adults. In animals deafened as adults there was a 50% reduction in the number of neurons activated by electrical stimulation of the cochlea, compared with studies in normal controls. In contrast, very few or no responses could be elicited from IC of animals made deaf from birth. The results imply that lack of neural activity in the ascending auditory pathways from an early stage of development results in an inactivation of IC neurons. It is not clear if such inactivity is temporary or permanent.
Amikacin↗
Three dimensional intracellular structure of the cochlea using the A-O-D-O method.
We have observed the three-dimensional intracellular structure of the organ of Corti and the spiral ganglion of the chinchilla using the aldehyde osmium-DMSO-osmium (A-O-D-O) method, and compared our findings with previous studies in the guinea pig. In cells of the spiral ganglion and the organ of Corti, microtubules and other cytoplasmic filaments were digested by OsO4 during processing to provide a clear view of membranous structures such as mitochondria, endoplasmic reticulum and Golgi apparatus. The appropriate digestion time with 0.1% OsO4 solution was found to be 60-80 hrs for the organ of Corti and 70-90 hrs for the spiral ganglion. The A-O-D-O method has been especially useful to show the organization of the endoplasmic reticulum of the outer hair cells. We have made a further characterization of the apical cistern, a reticular network which appears to link the basal body of the (vestigial) kinocilium to both Hensen's body and the subsurface cisternae.
Animals↗
Correlation of cochlear pathology with auditory brainstem and cortical responses in cats with high frequency hearing loss.
Newborn kittens were treated with the aminoglycoside amikacin to produce a bilateral high frequency cochlear hearing loss. The degree and stability of hearing loss were confirmed by recording auditory brainstem evoked potentials (ABR audiograms). After maturation, cochleotopic frequency representation within primary auditory cortex (AI) was mapped using standard microelectrode recording techniques. The cochlear sensory epithelium was assessed with SEM and the pattern of damage compared with the ABR audiograms and cortical frequency maps. Amikacin treatment resulted in various patterns of haircell damage towards the base of the cochlea. A relatively abrupt transition between damaged and undamaged haircell regions resulted in an ABR audiogram with normal threshold to low frequencies and a high frequency elevation with a steep cut-off slope. In the cortical map, low frequency representation was normal, but anterior areas contained only neurons tuned to a common frequency which corresponded to the frequency-place position of the boundary of the haircell lesion and to the cut-off frequency of the audiogram. A large transitional zone of the cochlear lesion correlated with a gradual cut-off slope to the audiogram and again a remapping of the anterior and normally high frequency area to a common lower frequency. Haircell loss or damage (i.e. disarray of stereocilia) in lower frequency regions of the cochlea correlated with a significant reorganization of the lower frequency bands in the cortical map. We conclude from this study that the pattern of cochleotopic organization of the cortex is dependent on the pattern of activity in the ascending sensory pathway during early stages of development.
Amikacin↗
Autoimmune inner ear disease: a review of basic mechanisms and clinical correlates.
Otolaryngologists have long sought to identify causes of sensorineural hearing loss that might be reversed by medical treatment. One such entity has become known as autoimmune inner ear disease. The potential improvement in auditory function in these patients subsequent to immunosuppressive therapy has created a desire in clinicians to better understand this disease. This paper begins by reviewing the basic concepts of autoimmunity. The experimental and clinical data concerning autoimmune inner ear disease are then described and analyzed. Finally, conclusions are drawn concerning our current state of understanding of this disease process.
Animals↗
Influence of acoustic deprivation on recovery of hair cells after acoustic trauma.
The purpose of this study was to investigate how the recovery of the cochlea, after acoustic trauma, might be influenced by acoustic stimulation or deprivation. In anaesthetized adult chinchillas, both ears were simultaneously exposed to a traumatizing acoustic stimulus (2 kHz tone, at 117 dB SPL for 15 min). Probe microphones positioned in both bullae were used to ensure identical exposure to the two ears; this was important because the experiment relies on within-animal controls. Cochlear action potential thresholds across frequency (CAP audiograms) were used to verify the similarity of threshold shifts to the two ears. Immediately following, a unilateral ossiculectomy was performed which resulted in one cochlea being acoustically deprived during the recovery period, whilst the other was not. In groups of animals with recovery periods of 1, 3, 6, and 12 weeks, both the acoustically deprived and the normally stimulated cochleas were examined with scanning electron microscopy. To quantify hair cell damage, we used a damage scale based on stereociliar integrity; for each cochlea, a standard region 5.5-8.5 mm from the apex was studied in detail. We found that after acoustic trauma, hair cell damage to the cochlea which is deprived of sound during the recovery period, is significantly greater compared with that in the normally stimulated, contra-lateral cochlea. Our results suggest that mechanical activation of the inner ear acts to inhibit long-term degenerative processes, or influence repair of partially damaged hair cells.
Action Potentials↗
Cochlear nerve action potentials during cerebellopontine angle surgery: relationship of latency, amplitude, and threshold measurements to hearing.
The cochlear nerve action potential (AP) was monitored during two types of hearing preservation surgery: (1) removal of small cerebellopontine angle tumors and (2) retrolabyrinthine vestibular nerve section. The purpose of this investigation was to study the relationship between changes in intraoperative AP latency, amplitude, and threshold, and changes in audiometric hearing subsequent to surgery. The presence or absence of a cochlear nerve AP toward the end of surgery accurately predicted the presence or absence of postoperative hearing (93%). Regression analyses were performed to explore the relationship between audiometric pre- and postoperative thresholds and intraoperative AP threshold changes. Statistically significant relationships were found for (1) pre-operative audiometric thresholds and initial AP click thresholds (p = 0.0068); (2) final AP click thresholds and postoperative audiometric thresholds (p = 0.0003); (3) AP click (p = 0.0001) and 1 kHz (p = 0.0006) threshold shifts and pre- to postoperative audiometric threshold shifts. No relationship was found between either AP latency or amplitude values and postoperative hearing. Intraoperative threshold measurements can be used as an indicator of hearing change. If amplitude or latency indices are also monitored it may be better to use stimulus levels close to the evoked potential threshold.
Action Potentials↗
Some aspects of temporal coding by single cochlear fibres from regions of cochlear hair cell degeneration in the guinea pig.
Some temporal coding properties of cochlear nerve fibers are investigated in kanamycin-treated guinea pigs (GPs) with various degrees of outer hair cell (OHC) degeneration. In particular, the phase locking ability of fibres from pathological cochleas, and also their adaptation properties are compared with the properties of normal cochlear fibres. No systematic effects of OHC loss on these properties have so far been found. These preliminary results therefore suggest (in so far as these animals can be regarded as models of sensorineural hearing loss of cochlear origin in man) that little deterioration should be expected in functions purely dependent upon faithful temporal coding of the stimulus waveform.
Acoustic Stimulation↗
Cochlear fibre responses in guinea pigs with well defined cochlear lesions.
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Animals↗
Proceedings: Correlation between cochlear outer hair cell damage and deterioration of cochlear nerve tuning properties in the guinea-pig.
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Animals↗