Biomedical subjects

R V Oblap

Publications and source records attributed to R V Oblap.

7 recordsLinked to original sources

PubMedDate not supplied

[Genetic structure of local Ukrainian cattle breeds].

Analysis of 6 cattle breeds (5 local Ukrainian breeds and the Holstain breed) on the 9 polymorphous molecular-genetic markers (transferrin, ceruloplasmin, amylase-1, posttransferrin, receptor to vitamin D, haemoglobin, leptin, kapa-casein) was carried out. The rare allele of transferrin was revealed in two local breeds and the rare allele of leptin--in two another local breeds. Associations between syntenic loci (transferrin, ceruloplasmin, kappa-casein, receptor to vitamin D) were observed only in the local breeds but not in the Holstain one. Locus-specific conservation of the ancestor allelic variants in the local breeds and effect of selection on interloci associations are discussed.

Alleles↗

PubMedDate not supplied

[Comparative analysis of the genetic structure of Red Polish cattle in Poland and the Ukraine].

Comparative analysis of genetic structure of two groups of Red Polish cattle, which reproduce in Poland and Ukraine, was made. Six molecular-genetic markers (kappa-casein, beta-lactoglobulin, leptin, myostatin, growth hormone, and pituitary-specific transcription factor Pit-I) were tested by PCR-RFLP. No significant differences between the considered intrabreed groups were found. High frequency of some alleles (Csn kappa B, Blg B, and Gh L) related to the important productivity traits were observed. The rare alleles in some genes were revealed. The obtained results are evidence of the unique characteristics of the investigated breed.

Animals↗

PubMedDate not supplied

[Development of diagnostic kit "Ptakh-Grip PLR" for detection of highly pathogenic strain of avian influenza virus H5N1 by polymerase chain reaction].

A diagnostic kit for detection of avian influenza virus by real-time polymerase chain reaction was developed. This kit allows to identify the influenza viruses type A and highly pathogenic strain of avian influenza virus H5N1. The diagnostic kit is universal and adopted for ABI PRISM SDS (Applied Biosystems), RotorGene (Corbett Research) and iQCycler (BioRad) PCR machines.

Amino Acid Sequence↗

PubMedDate not supplied

[The bovine leukosis virus and the diagnosis of infected animals].

The results of selection of primers and amplification of fragments of provirus genes Gag and Env of bovine leukemia virus (BLV) in artificially infected cell line and in cattle leukocytes are presented. The distinctions between diagnoses of BLV infected animals by the testing their blood for antibodies to viral antigens and by the presence of provirus genes in DNA of their leukocytes were revealed.

Animals↗

PubMedDate not supplied

[Interlocus associations and their variability in cattle].

The results of analysis of interloci associations between two pairs of syntenic loci (transferrin and ceruloplasmin, receptor for vitamin D and kappa-casein) and two non-syntenic ones (amylase-1 and post-transferrin 2) in two cattle groups of Red Steppe breed (infected and uninfected by bovine leukosis virus) and in two groups of Black-and-White Holsteins (from relatively "pure" zone and from the 10 km zone of Chernobyl NPP) were presented. It is found that "linkage disequilibrium" between loci is observed independent of their synteny. The data obtained allowed the authors to suppose, that the interloci associations are rather controlled by different factors of artificial and natural selection than by the genetic linkages between genes.

Alleles↗

PubMedDate not supplied

[The intraspecific differentiation of Przhewalski's horse and the domestic horse by 5 molecular genetic markers].

Analysis of albumin, transferrin, receptor to vitamin D, esterase, alpha 1-beta glycoprotein polymorphisms in Przhewalski's horse, Orlov's and Russian trotters, Guzul and Yakutian domestic horse breeds was carried out. The data about similarity of intraspecies differentiation of Przewalski's horse's populations and interbreed distinctions were obtained. Locus-specific particularities of genetic structures of investigated animal groups were revealed.

Alleles↗

PubMedDate not supplied

[The phenotypic and genetic structure of the Ukrainian Grey breed of cattle].

A comparative analysis of phenotypical characteristics, the polymorphism of erythrocyte antigens and genetic-biochemical systems in the groups of Ukrainian Grey cattle, bred in Ukraine and Russia was carried out in different years. Data have been obtained on peculiarities of genetic structure of Ukrainian Grey cattle and on certain breed distinctions in the space and time.

Animals↗