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R Vanni

Publications and source records attributed to R Vanni.

At least 55 records · Page 3Linked to original sources
PubMed1992

Uterine leiomyoma cytogenetics. III. Interphase cytogenetic analysis of karyotypically normal uterine leiomyoma excludes possibility of undetected trisomy 12.

Uterine leiomyoma, a benign tumor that histopathologically is rather homogeneous, was recently characterized cytogenetically. About 40% of the investigated tumors are associated with clonal chromosome abnormalities and five different subgroups have been identified, characterized by trisomy 12, t(12;14)(q14-15;q23-24), del(7q), t(1;2)(p36;p24), and 6p rearrangements. In our survey of 76 cases, trisomy 12 was observed in 10% of the abnormal cases. To exclude a possible underscoring of this abnormality, we reexamined 15 of the cases with normal karyotype by interphase cytogenetics using a chromosome 12 alphoid DNA probe.

Chromosomes, Human, Pair 12↗
PubMed1991

Uterine leiomyoma cytogenetics. II. Report of forty cases.

Chromosome analysis of 40 cultured uterine leiomyomas revealed the presence of clonal changes in 32.5% of them, confirming the cytogenetic heterogeneity within this type of tumor, mostly referable to a few cytogenetic subgroups. Preferential involvement of 12q14-15 and 14q23-24 bands in reciprocal and complex translocations was most commonly observed. Deletions of chromosome 7 and changes of chromosomes 1, 2, and to a lesser extent, chromosomes 19 and 22 were also found. Constitutional karyotype of patients bearing tumors with karyotypic abnormalities was examined. In one patient, two cells were found with t(12;14)(q14-15;q23-24) translocation and two with del(14)(q13q23-24). The latter rearrangement was also present as a clonal change in the tumor.

Chromosome Deletion↗
PubMed1989

Uterine leiomyoma cytogenetics. I. Rearrangements of chromosome 12.

Cytogenetic investigation on 19 benign tumors of uterine smooth muscle was carried out after short-term cultures. Clonal chromosome abnormalities were present in four cases; 15 had normal karyotypes. All four cases with abnormal karyotype showed changes of chromosome 12. In three cases, chromosome 12 was involved in structural rearrangements with chromosome X, 1, and 4, respectively. Breakpoints on this chromosome occurred in different regions. The fourth case had trisomy 12 and an extra deleted chromosome 2, del(2)(p22). These findings confirm the involvement of chromosome 12 in uterine leiomyoma.

Chromosome Aberrations↗
PubMed1989

Analysis of human metaphase chromosomes using antibodies to double-stranded and single-stranded DNA: staining patterns are related to DNA conformation.

Fresh and 6-day-old fixed chromosome spreads, both untreated and treated with various banding techniques and nucleases, were stained using monoclonal antibodies to double-stranded and single-stranded DNA. DNA in fixed chromosome preparations became progressively denatured with ageing. The staining pattern of untreated chromosomes with anti-double-stranded DNA antibodies (which resembles G-banding) was determined by the conformation of the chromosomal DNA.

Antibodies, Monoclonal↗
PubMed1988

Analysis of electric and magnetic fields leaking from induction heaters.

Results are presented of an investigation on electric and magnetic fields leaking from inductive (magnetic) heaters that are used for thermal processing of high-power electron tubes and lasers in an industrial plant. Measurements of electric and magnetic fields were done using both commercially available and laboratory-developed instrumentation. Isotropic H-field sensors were developed to allow quantitative evaluation of high-intensity magnetic fields. Ten induction heaters with nominal A.C. power ranging from 2.5 kW to 15 kW and operating at frequencies between 300 kHz and 790 kHz were surveyed. Electric field strengths up to 8 kV/m and magnetic field strengths up to 20 A/m were measured.

Environmental Exposure↗
PubMed1988

Cytogenetic investigation on 30 bladder carcinomas.

Cytogenetic study of 30 bladder carcinomas confirmed the heterogeneity and the complexity of the karyotypic picture in this type of tumor. Presence of numerical and/or structural chromosome aberrations was observed in all tumors. Clonal abnormalities were found in 19 cases. Chromosomes most frequently involved in changes were chromosome #1, #3 and #11(36.6%, 26.6%, and 20% of the cases respectively). Trisomy 7 and monosomy 9 were the sole abnormalities in one case each.

Chromosome Aberrations↗
PubMed1988

Effect of HpaII and MspI restriction endonucleases on chronic myelogenous leukemia chromosomes. Detection of CpG dinucleotide demethylation in situ.

The restriction endonucleases HpaII and MspI both cleave the nucleotide sequence CCGG, but the action of HpaII is inhibited if the internal cytosine is methylated. HpaII and MspI were used on fixed chromosomes from bone marrow cells of individuals suffering from chronic myelogenous leukemia and healthy individuals. We found that MspI acts with the same efficiency on all chromosome samples, whereas HpaII extracts more DNA from the chromosomes of leukemic individuals than from the chromosomes of nonleukemic individuals. We postulate that demethylation of cytosine in the CpG dinucleotide of leukemic cell DNA accounts for our findings.

Cytidine Monophosphate↗
PubMed1988

A specific translocation, t(12;14)(q14-15;q23-24), characterizes a subgroup of uterine leiomyomas.

We have cytogenetically investigated short-term cultures initiated from 34 uterine leiomyomas, all of which were histologically completely benign. Clonal chromosome abnormalities were detected in five cases, a normal female complement in 22, whereas, in the remaining seven tumors no karyotype could be established. Apparently identical reciprocal translocations, t(12;14)(q14-15;q23-24), were found as the sole abnormality in four tumors. The fifth abnormal case contained a t(2;14)(p11;p11). We conclude that chromosome aberrations may be found in myomas of the uterus, and that t(12;14)(q14-15;q23-24) characterizes a subset of these tumors.

Chromosome Banding↗
PubMed1988

Transitional cell carcinoma of the bladder in a young man. A multidisciplinary approach.

A multidisciplinary diagnostic approach to a case of bladder carcinoma in a 19-year-old male smoker is presented. The transitional cell carcinoma was submitted to conventional histological examination, flow cytometry and cytogenetic analysis. Serum and urine tumour markers were also investigated. The tumour was diploid, with an increased proliferative phase, and a chromosome marker was found. A net decrease in serum and urine ferritin concentrations was noted after transurethral resection of the neoplasm and its low malignancy was confirmed at follow-up.

Adult↗
PubMed1988

Ageing of fixed cytological preparations produces degradation of chromosomal DNA.

When fixed chromosome preparations were allowed to age for 1-72 h, they became progressively more susceptible to digestion by exonuclease III and by S1 nuclease. Analysis of DNA from these aged preparations on agarose gels showed that the molecular weight of the DNA decreased as ageing progressed. We conclude that DNA in fixed chromosome preparations becomes progressively degraded as the preparations age.

Cell Survival↗
PubMed1987

Cytogenetics of bladder cancer: rearrangements of the short arm of chromosome 11.

The presence of nonrandom chromosomal defects has been reported in a number of leukemias, lymphomas, and solid tumors. Primary karyotypic changes have been described in bladder carcinoma. We report the finding of chromosomal derivative markers 11 in three cases of bladder cancer. These markers were observed in all interpretable metaphases obtained by a direct method. The involvement of the short arm of chromosome 11 in the carcinogenetic process is discussed.

Aged↗
PubMed1986

Exposure of workers to intense RF electric fields that leak from plastic sealers.

This paper presents the results of an environmental investigation in a plastic-ware industry using RF sealers. Environmental measurements have demonstrated the presence of strong electric fields the intensity of which: reaches significant levels from the health-protection point of view only in proximity to the applicators; often exceeds the maximal levels allowed by all standards (including the most permissive) for short duration exposures, especially in the region of the hands. Results of a cross-sectional study on the health of 63 female workers are related to the observed RF exposure. A statistically significant correlation was found to exist between RF exposure and some minor health effects--eye irritation, upper limb paresthesias, and vitreous body disorganization.

Equipment Safety↗
PubMed1986

Identification of marker chromosomes in bladder tumor.

Sequential staining with Giemsa and quinacrine mustard on direct cytogenetic preparations was employed to characterize the karyotype of five bladder tumors of different stage and grade (TNM classification). The use of QFQ-banding allowed: the characterization of 2 euploid and 1 pseudodiploid cases, and the identification, in the two hyperdiploid cases, of a number of rearranged chromosomes otherwise morphologically indistinguishable from the normal ones. The importance of banding technique on direct cytogenetic preparations is stressed.

Adult↗