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Biomedical subjects

R Varró

Publications and source records attributed to R Varró.

At least 19 recordsLinked to original sources
PubMed1985

[Immunoenzyme analysis method for detecting anti-Pseudomonas aeruginosa antibodies in persons inoculated with pyoimmunogen].

The possibility of using the indirect ELISA techniques for evaluating the level of the post-vaccinal production of humoral antibodies in donors immunized with Pyoimmunogen. P. aeruginosa vaccine, has been studied. The specificity and high resolution of this test system, based on the immobilization of the antigens of the vaccinal preparation on a solid-phase carrier, have been demonstrated. A rational method for the evaluation of specific antibody titers with due regard to the spectrophotometric data indicating the results of the reaction and the degree of the dilution of the serum under test has been proposed.

Antibodies, Bacterial↗
PubMed1980

Interaction of targeted liposomes with human peripheral mononuclear blood cells and lymphocytes of chronic lymphoid leukaemic patients.

Association of small unilamellar vesicles (SUV) can be effectively targeted to normal human peripheral mononuclear blood cells (PMBC). - Lymphocytes of chronic lymphoid leukaemic (CLL) patients show a low rate of liposome association, which cannot be improved by targeting with anti-lymphocyte globulin (ALG). Presence of serum evoked an enhanced association of liposomes to PMBC.

Animals↗
PubMed1980

The effect of transfer factor on spontaneous shedding of sheep red blood cell binding receptors of T lymphocytes in vitro.

Many effects of transfer factor can be used for testing of its activity in vitro. Its effect on rosette formation has been utilized in two methods: the enhancement of rosetting of trypsin-treated T lymphocytes and the increase of 'active' rosettes depressed under some immunopathological conditions. 'Active' rosetting lymphocytes of healthy blood donors if kept at 37 degrees C for 4 hr shed partly their sheep red blood cell-binding receptors into the culture medium supplemented with 25% fetal calf serum. The adding of the negative skin test-converting fraction of dialysable leucocyte extracts inhibits the decrease of the number of rosettes. Possible explanations for the observed phenomenon are: transfer factor increases the rate of receptor synthesis, it causes uncovering or redistribution of the receptors, or it stabilizes otherwise shed membrane structures.

Animals↗
PubMed1979

Synthesis and studies of transferrin.

Combination of the usual techniques yielded 70-80 p.c. pure apo- and iron-transferrin preparations, whose analysis revealed the presence of antigenically identical transferrin fractions differing from one another in respect of electrophoretic motility and sedimentation contrast. No similar modifications of transferrin have previously been reported.

Electrophoresis, Agar Gel↗
PubMed1979

Characterization of enhancing factor (EF) prepared from spleen of mice immunized with tetanus toxoid.

In cultures of spleen cells from tetanus toxoid-primed mice a soluble, nonspecific material appeared, which enhanced antibody response in vivo. The active material was purified by gel filtration on Sephadex-G-150 and by affinity chromatography on Con-A lectin. According to the immunological and physiochemical investigation the active material does not contain carbohydrate and its molecular weight is in the 100 000 dalton range.

Animals↗
PubMed1979

Hydrophobic interaction of extracellular protein A from Staphylococcus aureus with octyl-sepharose CL-4B.

Extracellular Protein A from Staphylococcus aureus was shown to bind to alkyl agarose adsorbents of different carbon chain length. Octyl-Sepharose CL-4B was chosen to purify the active material, as the strong interaction with this adsorbent allowed the separation of Protein A from the bulk of the contaminants. Protein A retained its ability to bind to IgG in the presence of up to 3.5 M ethylene glycol as demonstrated by hemagglutination. A theory on the possible involvement of hydrophobic interaction in the binding reaction of Protein A to IgG is advanced.

Hemagglutination Tests↗
PubMed1978

Separation of beta2-microglobulin from transfer factor in dialyzable leukocyte extracts.

Dialyzable leukocyte extracts prepared according to the original method of LAWRENCE contain 4 X 10(4) molecules of beta2-microglobulin per lymphocyte equivalent. The negative skin test converting biological activity (transfer factor) is separable by means of Sephadex G-25 gel chromatography from the beta2-microglobulin component of the extracts. This finding does not support the hypothesis of SHIFRINE and SCIBIENSKI on beta2-microglobulin being the nonspecific anchor of specific transfer factor to nonsensitized lymphocytes.

Beta-Globulins↗
PubMed1975

Application of pepsin-digested horse antibodies for quantitative immunoelectrophoresis.

The majority of the precipitating antibodies in hyperimmune horse serum belong to the beta globulins, as demonstrated by reversed immunoelectrophoresis. As these antibodies migrate in agarose gel during electrophoresis in conventional pH = 8.6-8.9 buffers, horse antiserum cannot be used satisfactorily for quantitative immunoelectrophoresis. On pepsin digestion of horse antiserum F(ab)' 2 fragments are generated which migrate with the gamma globulins. This cleaved product works well in quantitative immunoelectrophoretic techniques.

Animals↗
PubMed1975

Biochemical, immunochemical and immunobiological properties of a chloramphenicol-resistant Salmonella typhi strain isolated in Mexico.

The authors have compared the biochemical, immunochemical and immunobiological properties of a chloramphenicol-resistant S. typhi strain isolated during the typhoid fever epidemic in Mexico of 1972 and those of a chloramphenicol-sensitive strain (S. typhi Ty2). They have found no difference in the chemical composition of the two strains. The immunobiological investigations have shown, that the chloramphenicol-resistant strain contains less Vi antigen, it is less virulent on mice, and its active and passive mouse-protective ability is lower than that of the chloramphenicol-sensitive S. typhi Ty2 strain. These findings supported by the result of the electrophoretic analysis suggest that the chloramphenicol-resistant strain is a VW strain, while the chloramphenicol-sensitive strain is a full V strain; the quantitative difference found between the immunogenicity of the two strains to the advantage of the chloramphenicol-sensitive strain may probably be explained by this fact. On the basis of their immunological investigations the authors are of the opinion, that vaccines prepared from chloramphenicol-sensitive S. typhi strains (provided these are full V strains, as e.g. the S. typhi Ty2 strain) will protect most likely also man against infection due to chloramphenicol-resistant S. typhi strains.

Agglutination Tests↗
PubMed1975

Behaviour of polyclonal human IgE in the course of fractionation.

Normal human pooled plasma was fractionated by a variety of methods. The IgE concentration of the different fractions was determined by a solid-phase radioimmunoassay. The results of these studies indicate, that polyclonal IgE behaves similarly to IgE of myeloma origin. A biospecific method was worked out to purify IgE from fraction III of the cold ethanol fractionation procedure.

Chemical Fractionation↗
PubMed1975

The effect of enhancing factor on the immunity of mice against tetanus.

Enhancing factor was prepared from spleens of mice primed and reinjected with tetanus toxoid. The culture supernatant was separated by gel chromatography on Sephadex G-150, the resulted fractions were ultrafiltered and their enhancing activity was tested in vivo. The 1st fraction separated by gel chromatography proved to be active.

Animals↗
PubMed1975

The application of polyvalent horse immune sera for electroimmunodiffusion methods.

Horse immune sera do not give satisfactory results in immunochemical techniques based on electrophoresis of antigens through antibody-containing agarose gel. As the majority of precipitating horse antibodies belongs to the beta globulins, they migrate in the gel during electrophoresis. After enzymatic treatment the pepsin fragments work well in all electroimmunodiffusion methods.

Animals↗