Autofluorescence of the ischemic skeletal muscle.
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Biomedical subjects
Publications and source records attributed to R Vasiu.
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The study of nuclear parameters (long and short nuclear axes, nuclear axes ratio, nuclear area) revealed differences both between the primary breast tumors and their lymph node metastases and between the lymph nodes isolated from the same axilla. The results are discussed in terms of tumor progression.
The comparative study of the percentage nuclear area in brain tumors by the point-counting technique and digital analysis showed a good correlation between the results obtained. The investigation suggests the possibility of using the volume corrected mitotic index to tumors with uniformly distributed cells.
The authors report a case of composite tumor of the gallbladder which exhibited features of both adenocarcinoma (with typical areas, signet ring cells and clear cells) and endocrine cell carcinoma. The histological picture suggests the origin of both components in a common primitive cell with the capacity to differentiate to different types of metaplastic epithelium and supports the hypothesis of a sequence metaplasia--dysplasia--carcinoma.
A case of thyroid carcinoma with a triple medullary, folliculo-papillary and squamous differentiation is reported. Lymph node metastatic deposits showed folliculo-papillary and squamous cell areas. The problems of histogenesis are discussed.
A case of gastric Burkitt's lymphoma with pancreatic infiltration in a 15-year-old boy is reported. Local lymph nodes were not involved. Review of the literature disclosed that the reported cases of Burkitt's lymphoma were localized in the intestine. The origin, evolution and prognosis of gastrointestinal lymphoma are briefly discussed.
The DNA nuclear content of apocrine cells was studied in 5 cases of fibrocystic disease, 3 cases of invasive duct carcinoma and 3 cases of invasive lobular carcinoma. Both diploid and aneuploid values were found in each group. The possible significance of apocrine change in breast lesions is discussed.
The authors have used the point counting method for the morphometric assessment of the histological aspect of laryngeal carcinoma. The results suggest that subjective histopathological grading tends to overestimate the tumor grade. The implications of these data for the prediction of prognosis and selection of therapy are briefly discussed.
Three cases of malignant myxoid melanomas are described. That feature may simulate other malignant tumors with myxoid changes, especially in amelanotic metastases. The problems of differential diagnosis are briefly discussed.
Cytophotometric study of suspicious (group III of the Papanicolaou's classification) smears shows the heterogeneity of this group, which includes cases with diploid--tetraploid DNA modes, cases with a wide distribution of DNA values and aneuploid cases. The significance of these findings in relation with the biological properties of cells is briefly discussed.
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The parallelism between hypoxic changes in the myocardium revealed by Lie's technique and by fluorescence microscopy and the ionic changes (Na+, K+, Ca2+) was studied in 51 autopsy cases. The Lie technique was positive in 52.6 per cent of the cases of cardiosclerosis with myocardial infarction and in 15 per cent of the cases of cardiosclerosis without infarction, while fluorescent areas were found in all cases of infarction and in 35 per cent of cardiosclerosis cases without infarction. Na+ and K+ were decreased and Ca2+ ions were increased in both ventricles and in both sexes, both in infarctions and in cardiosclerosis cases without infarction. Ionic changes are more probably related to the intensity of cardiosclerosis rather than to age or sex.