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R Verbruggen

Publications and source records attributed to R Verbruggen.

5 recordsLinked to original sources
PubMed1991

In-target production of [13N]ammonia via proton irradiation of dilute aqueous ethanol and acetic acid mixtures.

A reliable, reproducible method has been developed for the in situ production of decicurie quantities of [13N]ammonia by proton irradiation of pressurized, dilute aqueous solutions of acetic acid and ethanol. Some of the parameters investigated for their effect on the 13N product distribution included dose, dose rate, solute concentration, target body material, beam strike volume and proton energy. For the conditions investigated, [13N]NH3 was produced directly in the target in yields greater than 75%.

Acetates↗
PubMed1977

Analysis of murine C-type virus structural proteins by rocket and crossed immunoelectrophoresis.

We have examined the structural proteins of Rauscher murine leukemia virus (R-MuLV) by means of rocket immunoelectrophoresis and crossed immunoelectrophoresis, using polyspecific antisera to Tween/ether-disrupted purified R-MuLV. Fifteen different precipitation lines were recognized in virus lysates. Using five reference antisera prepared to purified R-MuLV-structural proteins, the precipitation lines of p 10, p 15, p 30 and gp 69/70 were identified. These techniques, although less sensitive than radioimmunoassay, have several advantages, such as simplicity, direct control of precipitation reactions and possibility of using crude antigen preparations.

Animals↗
PubMed1975

Subtilopeptidase A isoenzyme system. Interaction with serum components and its importance for quantitative immunoelectrophoresis.

A method was developed which involved electroimmunoassay and crossed immunoelectrophoresis of subtilopeptidase A (EC 3.4.21.14). Initial trials with unfractionated antiserum were not successful and interaction of the enzyme with non-immunoglobulin serum components were shown to be the cause of the failures. Quantitative immunoelectrophoresis was possible when purified immunoglobulins were used. A pH of 6.5 (lower than the usual pH 8.6) was necessary to obtain a proper baseline definition. Subtilopeptidase A was confirmed as a multiple isoenzyme system. Qualitative inter-batch variations were detected. Di-isopropyl phosphorofluoridate inhibition altered the electrophoretic pattern, but no loss of antigenic determinants was observed.

Animals↗