PubMed Health⌕ Search

Biomedical subjects

R Vitelli

Publications and source records attributed to R Vitelli.

12 recordsLinked to original sources
PubMed1996

Molecular cloning and expression analysis of the human Rab7 GTP-ase complementary deoxyribonucleic acid.

Rab7 is a small GTP-ase localized on late endosomes, which regulates late endocytic membrane traffic in mammalian cells. Moreover it has been shown that this protein has a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. We report here for the first time the isolation of a cDNA encoding human Rab7 from a placenta cDNA library. The open reading frame for human Rab7 encodes a protein of 207 amino acids which exhibits high homology with the mouse, rat, and dog counterparts. Northern blot analysis of total RNAs isolated from different cell lines with a cDNA probe containing the entire open reading frame revealed two mRNA transcripts of 2.5 and 1.8 kilobases. The isolation of human Rab7 cDNA will allow further characterization of its function in normal and pathological states.

Amino Acid Sequence↗
PubMed1996

[Postpartum psychoses. A case report].

The puerperal period is a critical one in the life of a woman, due to the numerous changes which occur at biological and psychological levels and also at the level of relationships. Various disturbances of a psychiatric nature may emerge during this period which represents a "risky" phase from a psycho-pathological point of view. Among some of these disturbances, so-called "puerperal psychoses", there is an uncertain nosographic connection. According to some authors, these psychoses could be distinct nosographic events, while others prefer to include them in psychotic disturbances which have already been classified. From this point of view the puerperal period would be a stressful event. Risk factors would be a first birth, previous psychotic puerperal episodes, inherited serious psychiatric disturbances and caesarean section. The therapy used in the case of such disturbances must take into account many aspects and implications which don't exclusively concern the new mother but which also look significantly at the newborn baby, and the patient's family circumstances. All this implies the necessity for a close collaboration between specialists in different medical areas (psychiatry and obstetrics). Particular attention should be given to the type of disturbed relationship which can occur between the mother and the new born child. The analysis of our case has stimulated many reflections from a clinical, therapeutic and prognostic point of view, and as a result we tent to agree with published material on the subject.

Adult↗
PubMed1995

Cloning and expression analysis of the murine Rab7 cDNA.

A cDNA clone coding for the Rab7 protein was isolated from an NIH3T3 cell line (mouse fibroblasts) cDNA library. Sequence analysis shows high homology to the rat and dog cDNAs. Northern blot analysis showed the presence of two messenger RNA that differ at the 3' untranslated region.

3T3 Cells↗
PubMed1993

[An algorithm for the analysis of the circadian profile of the arterial pressure in normotensive subjects].

In this paper we proposed the use of a spectral analysis technique, the fast Fourier transform (FFT), for the evaluation of the normal circadian blood pressure rhythm. By means of this algorithm it is possible to separate the slower and reproducible variations of the blood pressure profile (probably due to endogenous rhythm) from the faster and random ones (due to psychophysical status of the subject). The FFT technique is used for the analysis of ambulatory blood pressure monitorings obtained in 66 normotensive subjects (38 males and 28 females, mean age 35 +/- 10 years). Taking into account the mean duration of pressure variations related to major daily activities (e.g. sleeping, eating, etc) it is proposed to consider the first 10 harmonics as those having the main influence on the circadian blood pressure rhythm. The amplitude of the first 10 harmonics of the whole sample was evaluated by using the chi 2 and the Kolmogorov tests, and the results confirmed the assumption of a normal distribution of the amplitudes. The 95% confidence interval of these harmonics was chosen as the criteria for defining a normal range for the circadian blood pressure rhythm. This approach was tested in a group of 4 hypertensive patients showing that alteration of the circadian blood pressure rhythm can be easily diagnosed.

Adolescent↗
PubMed1988

In vitro motility of non-pregnant rat uterine horns.

The in vitro spontaneous mechanical and electrical activity of the non-pregnant rat uterine horn was investigated. An isometric isovolumic preparation was used to obtain records of intraluminal pressure, axial force, extracellular electrical activity and horn morphology. Mean intraluminal pressure was 28.6 cmH2O and mean force 2.5 g. Burst electrical activity was initiated at the ovarian end in 70% of the cases. Detailed analysis of electrical and mechanical events during a single contraction showed a preparatory phase involving the longitudinal muscle layer, followed by a stronger contractile event involving both longitudinal and circular layers. Moreover, the mechanical activity was modulated at the frequency of a component (0.16-0.20 Hz) of the slow electrical activity of the horn. Burst electrical activity appeared at five equidistant electrodes along the horn with variable delays: the highest delays (mean values 7.4 s for burst activity initiated at the ovarian end and 6.5 s at the vaginal end) were observed in the middle part of the horn. The spontaneous contraction of the segment appeared to be due to the activation of separate regions of the horn (i.e. an ovarian and a cervical region), more than to a propagated excitatory action.

Action Potentials↗
PubMed1987

Kinetic and survival response of the M14 cell line to lonidamine associated with adriamycin or hyperthermia.

Lonidamine (LND), an indazole-carboxylic acid derivative, was delivered alone and together with adriamycin (ADM) or hyperthermia to the human melanoma cell line M14, and cell survival was assessed. Cell cycle-specific effects were investigated by analyzing sequences of DNA content histograms by means of a suitable mathematical procedure. LND delivered for 1 h at a dose of 50 micrograms/ml did not affect proliferation and survival of the cells. Exposure of the cells for 1 h to ADM (1.0 microgram/ml) followed by LND for 1 h (50 micrograms/ml) produced the highest effect on the survival. Kinetic parameters were affected by the combined treatment slightly more than by ADM exposure alone. Simultaneous delivery of LND (50 micrograms/ml) with hyperthermia (42 degrees C, 1 h) reduced the survival and enhanced the block of cells in the G2M phase, as compared with the heat treatment alone. The effect of the treatments on cell survival appeared to be related to the perturbation of the G2M phase of the cycle.

Antineoplastic Agents↗
PubMed1984

Analysis of DNA synthesis rate of cultured cells from flow cytometric data.

The rate of DNA synthesis along S phase is estimated from flow cytometric histograms on the basis of a mathematical model of a cell population. In the absence of loss, the model expresses the population kinetics in terms of DNA synthesis rate, S-phase influx, and population size. A single histogram is sufficient to determine the DNA synthesis rate when the population is in balanced exponential growth. Two suitably chosen histograms are necessary if the S-phase influx is exponential in a time interval longer than the S-phase duration. The analysis procedure was tested on published autoradiographic data and applied to three cultured cell lines (CM-S, 3LL, and M14 cells) that show various patterns of DNA distribution. In each case the cell-cycle fractions, the DNA synthesis rate, and the S-phase duration were obtained.

Animals↗
PubMed1983

A general expression for sequential DNA-fluorescence histograms.

A general expression for time sequences of DNA-fluorescence histograms from flow microfluorometry is given in this paper. Such expression is given in terms of the law of DNA growth along S phase, the flux of cells into S, and the efflux out of M. Special conditions of growth (e.g. the exponential steady-state growth), and the case of blocks in S phase are also analyzed. Some simulations of the expressions obtained are presented.

Cells, Cultured↗
PubMed1983

Estimation of cell DNA synthesis rate from flow-cytometric histograms.

The rate of DNA synthesis along S-phase can be estimated on the basis of a simple model of a cell population, from a single FCM histogram under conditions of balanced exponential growth, and from two histograms suitably spaced in time under conditions of locally exponential S-phase influx. An algorithm that constructs a piecewise constant approximation of the synthesis rate and estimates the phase fractions from a single histogram is presented. The results of the application of the algorithm to a set of computer generated histograms in balanced exponential growth are reported.

Animals↗
PubMed1982

Relationship between trait anxiety and leisure time activities: a comparison of the unidimensional and multidimensional models of anxiety.

97 undergraduate students were given the State-Trait Anxiety Inventory, the S-R Inventory of General Trait Anxiousness, and a leisure activity inventory to test the hypothesis that the multidimensional model of anxiety could account for the variance in reported leisure activity more adequately than did the unidimensional model. Analysis showed a significant variation in overall leisure activity which correlated significantly with scores on the S-R Inventory measure of physical danger anxiety for both the 42 male and 55 female subjects. The findings which indicate support for the multidimensionaL model of anxiety are discussed and implications for future research noted.

Adult↗