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R W Atherton

Publications and source records attributed to R W Atherton.

5 recordsLinked to original sources
PubMed1979

A correlation between a spectrophotometric quantitation of rabbit spermatozoan motility and velocity.

Rabbit sperm motility was measured spectrophotometrically and a sperm motility index (SMI) was obtained. A comparative analysis between the SMI values and the velocity of motile sperm (obtained by time lapse photography of sperm tracks) was performed. The SMI specifically was compared to the mean velocity of the first 300 sperm cells observed and to the mean velocity of just the first 300 progressively motile sperm cells. In both comparisons, the SMI was highly correlated to the sperm track length (sperm velocity). In addition, a modification of the spectrophotometric procedure is described which allows measurements of the SMI to be made on semen extended into egg-yolk glycerine cryopreservation agents.

Animals↗
PubMed1975

An objective method for evaluating Angus and Hereford sperm motility.

Angus and Hereford sperm motility was evaluated by an objective spectrophotometric procedure and by a conventional subjective ranking system. Semen was collected by electroejaculation and divided so that one group contained 6 Angus samples and one group contained 12 Hereford samples. Objective procedures indicated that Angus sperm was twice motile as Hereford sperm in 2.9% sodium citrate. This objective procedure depends on the orientation of sperm in a flowing liquid and then spectrophotometrically measuring the sperm's ability to return to randomness when the flow is stopped. During preparation for freezing with liquid nitrogen, microscopic subjective ranking of sperm motility was carried out. Three conditions were studied subjectively: (I) arrival at the laboratory, (II) pre-freeze, and (III) thawed samples. No significant differences in subjective evaluation of sperm motility was found between conditions or breeds. Preliminary results indicate that this objective procedure can distinguish between the sperm motility in 2.9% sodium citrate from two breeds of cattle. Use of this objective procedure for studies relating to fertilization and artificial insemination is evident and there is no theoretical reason why the procedure can not be used with other species.

Animals↗