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Biomedical subjects

R W Else

Publications and source records attributed to R W Else.

51 records · Page 3Linked to original sources
PubMed1978

Antigens associated with canine spontaneous mammary carcinoma.

Soluble proteins were prepared from canine spontaneous mammary carcinoma (CSMC) cells by extraction with 3M KCl or limited hydrolysis of isolated cell membranes with papain. These preparations were assayed for tumour-associated antigens by inhibition of fully absorbed rabbit antisera. Organ-specific reactivity was demonstrated and antigenic specificity indicated the possible involvement of fetal components. Exclusion chromatography showed that antigenic activity was associated with macromolecules of different sizes in each of the protein preparations which allowed partial purification of tumour-associated antigens. Antigens were also prepared from circulating immune complexes in the serum of tumour-bearing animals. These studies provide a basis for the development of serological markers of malignancy in CSMC.

Animals↗
PubMed1977

Oestrogen receptors in canine mammary tumours.

One hundred and twenty-nine canine mammary tumours of varying histological types were examined for the presence of oestrogen binding protein by the dextran-coated charcoal method with the binding parameters of the reaction determined by Scatchard plots. By this method, 39 per cent of the 129 mammary tumours were shown to contain oestrogen receptor protein. This figure fell to 25 per cent for benign tumours and rose to 52 per cent for malignant tumours. There was no correlation between the histological nature of tumours and oestrogen receptor content in either benign or malignant forms. Oestrogen receptors were not demonstrable in normal mammary tissues. Whether the presence of oestrogen receptors in a tumour is indicative of hormonal dependence as is reported for human breast tumours is being investigated in the bitch.

Animals↗
PubMed1976

Oestrogen receptors in feline mammary carcinomas.

Twenty primary or metastatic felline mammary tumours were assayed for the presence of oestrogen receptors by the dextran-coated charcoal method. In only two of the tumours was the presence of specific oestrogen binding protein demonstrable. The high negative rate in comparison with human and canine mammary tumours assayed under similar conditions seemed to be a peculiar feature of the feline tumours and suggested a lack of oestrogen dependency. The possibility remains that other hormones may be concerned with the growth of feline mammary tumours and further work is in progress.

Adenocarcinoma↗
PubMedDate not supplied

Evaluation of B and T lymphocytes and plasma cells in colonic mucosa from healthy dogs and from dogs with inflammatory bowel disease.

The aim of this study was to investigate the subpopulations of lymphocytes in the colonic mucosa of healthy dogs and dogs with inflammatory bowel disease (IBD). Fourteen normal dogs and 13 dogs with IBD were examined. Endoscopic biopsy specimens of colonic mucosa from each dog were stained specifically for pan T lymphocytes (CD3) and pan B lymphocytes (CD79a), and for plasma cells with methyl green pyronin (MGP) stain. Cells were counted by means of a grid and statistical analysis was performed on the data collected. B and T lymphocytes were also counted in the glandular epithelium of normal dogs and dogs with IBD and the normal and abnormal groups compared statistically. Healthy dogs had significantly lower numbers of T cells in the lamina propria and glandular epithelium and significantly lower numbers of B cells in the lamina propria. Significant group differences for plasma cells were not evident. Our results indicate that in IBD a chronic cellular immune reaction is present in the diseased gut involving increased numbers of B and T lymphocytes.

Animals↗