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Biomedical subjects

R W Everett

Publications and source records attributed to R W Everett.

At least 19 recordsLinked to original sources

Multiplicative factors for estimation of daily milk and component yields from single morning or afternoon tests.

Prediction of daily yield from single a.m. or p.m. milkings requires factors that are the reciprocal of the proportion of total yield expected from single milkings given the milking interval. Further adjustments to estimated milk yield account for DIM. Factors used by the Cornell Dairy Records Processing Lab were estimated from data collected from August 1983 to November 1984. These factors appear to be biased. Inconsistent estimates of daily yield were observed monthly. New factors were developed using recent data. Factors from a.m. milkings for milk and protein yield were smaller than those currently in use. The reverse was true for fat yield. Covariants for DIM were larger than those currently used. Differences were observed when factors using data with known and assumed milking intervals were compared. Factors for a.m. milkings with known intervals were smaller than those from p.m. milkings with the same known intervals. Use of covariants for DIM were compared with covariants for single milk yield. The latter explained more variation in yield. Factors were tested on independent data. New factors with covariants for single milk yield performed best for estimation of total daily yield.

Animals

Genetic parameters of Italian brown Swiss for levels of herd yield.

Genetic parameters were estimated for yields and percentages of milk, fat, and protein for registered Italian Brown Swiss cows. Data were 72,690 mature equivalent 305-d first lactation yields split by herd average milk into four files. An expectation-maximization REML algorithm was used on a multiple-trait model with equal design matrices for fixed and random effects. Data were preadjusted for geographical area, age-month of calving, and days open. The model included herd-year and sire genetic group as fixed effects and sires as random effects. Estimates of heritability were .28, .30, .26, .42, and .34 for milk, fat, and protein yields, and fat and protein percentages, respectively. Genetic correlations of milk yield and milk component percentages were negative, as expected. Correlation of protein yield and protein percentage was null. Results show an increasing tend of variance components from low to high herd yields, indicating that dispersion of yield about the mean increases as average yield increases. Relative differences among environmental components were larger than those among genetic components, yielding larger estimates of heritability in herds with lower yield. Estimates of heritability for milk from low to high herd yields were .42, .38, .35, and .33.

Animals

Estimating milk, fat, and protein lactation curves with a test day model.

Test day models were used to estimate lactation curves for milk, fat, protein, fat percentage, and protein percentage and to study the influence of age, season, and herd productivity on Holstein lactation curves. Random effects of lactation within herd and fixed effects of herd test date were absorbed. Fixed effects of cow's age on test day and either DIM (57 divisions) by parity (1, 2, greater than or equal to 3) class or season of calving (winter or summer) by DIM by parity class were estimated. Lactation curves for yield traits derived from DIM solutions were flatter for first versus later lactation, even without addition of age effects. Differences between lactation curves for the two seasons were slight, suggesting that most observed seasonal differences are caused by seasonal productivity accounted for by herd test date effects. At peak, winter calving cows yielded slightly more milk of similar fat percentage but of lower protein percentage than those calving in summer. Data were also partitioned into nine subsets based on rolling herd milk and fat percentage. Lactation curves for yield traits, but not percentage traits, varied with rolling herd milk. Lactation curves for fat yield and percentage varied with rolling herd fat percentage.

Age Factors

Treatment of unusual Kock pouch urinary calculi with extracorporeal shock wave lithotripsy.

Since the establishment of extracorporeal shock wave lithotripsy for the treatment of upper urinary tract calculi, further potential applications have been explored. We report the successful use of extracorporeal shock wave lithotripsy for the treatment of obstructive calculi on staples within the afferent nipple of a Kock pouch ureteroileal urinary diversion.

Adult

Semen fertility--an evaluation system for artificial insemination sires, technicians, herds, and systematic fixed effects.

Semen fertility was evaluated on 4478 artificial insemination bulls using 2,007,520 breeding receipts for inseminations to 397,979 Holstein cows between January 1, 1978 and November 1, 1984 using mixed linear model methodology. Bulls differed on average by +/- 3.68% conception. Differences between herd-years ranged from 27.4 to -32.3% conception with an average difference of +/- 8.33%. Within herds, cow parity-service number accounted for the largest variation, ranging from 24.0% to -18.5% conception. June was the best month for conception (1.9) and January the poorest (-2.3), indicating effect of temperature and confinement in winter on estrous detection in the northeastern United States. Cows with intervals between repeat services of 21 d had conception rates 12% higher than intervals between repeat services of 11 d. Effect of month-year of semen collection varied by +/- 1.33% conception. Differences between technicians within years varied by +/- 3.37%. Results of the analysis indicate improved conception on dairy farms can be achieved by improved estrous detection, which is a major component of herd-year and service interval. Other components of the model contributed little to the variance in conception or are uncontrollable by farm management.

Animals

Effect of days carried calf, days dry, and weight of first calf heifers on yield.

Effects of days carried calf, days dry, and weight of first calf heifers were studied using records of Holstein cows processed at the Northeast Dairy Records Processing Laboratory between July 1980 and August 1984. Multiplicative factors were estimated for days carried calf for milk, fat, and protein using a model that adjusted for the age-month and herd-year of freshening. Factors developed show a close relationship between protein and milk with fat factors being smaller. Factors also are smaller than others reported in the literature. First lactation factors differed from second and third lactation factors. Analysis of days dry indicated that optimum number of days dry between lactations 1 and 2, 2 and 3, and 3 and 4 for maximized subsequent yield was 51 to 60 d dry for all lactations. Calculated F values showed greater significance for days dry than age-month of freshening. Optimum freshening weight of a first calf heifer to maximize first lactation milk yield is between 544 and 567 kg. The F values for weight at freshening were more significant than age-month of freshening.

Animals

Systematic environmental, direct, and service sire effects on conception rate in artificially inseminated Holstein cows.

Result of insemination was verified for 329,314 artificial inseminations by 882 service sires to 97,245 Holstein cows in 1,075 herds between May 1970 and December 1983. Estimates of systematic environmental and genetic components of cow and service sire components of conception rate were obtained preliminary to development of a fertility monitoring system. Conception rate was 2.9% higher in stanchion than loose housed herds and 2.3% higher in grade than registered cows. Fall months were superior to winter months, the greatest difference being 6.1% between October and January. Conception rate increased with herd milk production, decreased with both increased cow age, and increased herd size in mature and old cows. Regions and inseminators within regions were highly variable. Conception may be influenced by semen price; however, week day of insemination and duration of semen storage had no effect. Conception rate decreased for semen by bulls 8 yr and older, was lowest for semen harvested in June, but no season of collection effect was detected. No genetic trends for cow and service sire conception rate were found; heritability and repeatability were .08 and .06, respectively. Genetic correlations between cow and service sire conception rate and these components with first lactation production and semen output measures were all near zero. Therefore, the relationship between sire fertility and daughter fertility is near zero.

Animals

Phenotypic and genetic statistics of components of milk and two measures of somatic cell concentration.

Heritabilities, genotypic and phenotypic correlations for milk, fat, and protein yields, and two traits related to somatic cell concentration (cumulative lactation score and lactational somatic cell concentration) were estimated. A total of 18,416 first lactations of Holstein cows were analyzed by a new procedure for estimating variance components. Heritabilities were .21, .23, .19, .17, and .61 for milk, fat, and protein yields, cumulative lactation score, and lactational somatic cell concentration. Addition of protein yields to the current selection for two traits with nil economic value for protein would improve genetic gains for fat and milk yields in the northeastern United States. If cumulative lactation score and lactational somatic cell concentration were incorporated in current selection for two traits, restricted selection indexes should be used to avoid reduction in genetic gains for milk and fat yields.

Animals

Impact of genetic manipulation.

Genetic progress for production in dairy cattle is 50% or less of optimum. Manipulation of the reproductive process to increase genetic gains was reviewed. Economics of manipulating the reproductive process was evaluated by equating future discounted net income to current discounted expenses and solving for the break-even cost of the technique for 1 to n years the technique is employed. Techniques such as embryo transfer and sexed semen can be evaluated for economics. A system of splitting female embryos to produce performance-tested female genotypes was presented. The system has the capability of increasing genetic gains per year by 35%. The proposed system could be profitable if it is incorporated in the artificial insemination system and directed by professional geneticists.

Animals

Comparison of teat dips with differing iodine concentrations in prevention of mastitis infection.

Six commercial dairy herds were used to test the relative efficacy of three concentrations of iodine as a teat dip over 12 mo. Concentrations were .1, .25, and 1% iodine with free iodine contents of 3.5, 4.0, and 1.0 ppm. Two concentrations were compared in each herd. The greatest number of new infections (71) occurred in approximately 120 cows whose teats were dipped with the 1% iodine dip, and the fewest (52) occurred in a similar number of cow quarters dipped in .1% iodine; however, differences were not significant. Clinical mastitis was highest in the 1% group. If all clinical mastitis were the result of infection, even if bacteria were not isolated from the pretreatment sample or from samples collected at the start of the study and those quarters were added to the totals, then reduction of new infection with the .1% product would be significant.

Administration, Topical

Effect of overmilking on udder health.

This study evaluated the effect on udder health of extending the milking period with or without vacuum fluctuation. Eighty cows were allotted into treatment groups consisting of 1) Control milked with normal milking equipment and the milker removed as soon as milk flow ceases; 2) milked for 12 min with normal equipment; 3) milked 12 min with induced vacuum fluctuations; and 4) normal milking time with induced fluctuations. Groups 2 and 3 had more newly infected quarters than groups 1 and 4. However, there was no difference in the number of cows becoming infected. The increase in new infections is due to more quarters of already infected cows becoming infected rather than more new cows becoming infected. Extending the machine-on time appeared to increase the rate of cross infection presumably by lengthening the time of highest risk. Cows in groups 2 and 3 required more therapy of clinical mastitis which was a reflection of the higher new infection rate rather than of a change in trauma to the udder. Increasing the unit-on time had no apparent effect on external teat end condition.

Animals

Environmental influences on semen output.

Semen output characteristics of 7,858 ejaculates of 57 commercial Holstein sires in artificial insemination were analyzed to estimate effects of environment over 3-yr. Large differences existed between bulls, first and second ejaculates, and intervals between collections for all measures of semen output. Effects of age of bulls were greatest for sperm concentration per milliliter and total sperm output, peaking at 4 yr of age. Original semen volume was influenced less by age. December was the best month for total sperm production even though June and July were the months for most volume of seminal fluid production. Total sperm output was highest on Fridays and lowest on Wednesdays. Effects of temperature and humidity and changes in temperature and humidity on semen output were small and inconsistent. These results do not support the assumption that semen output could be increased in controlled environment conditions.

Age Factors

Effect of Dursban 44 on semen output of Holstein bulls.

Dursban 44, an insecticide for lice control, was applied to 185 Holstein bulls 9 to 52 mo of age. These sires were in various stages of progeny testing at an artificial insemination center. Application of this product killed 7 bulls, and the remaining bulls exhibited varying severity of illness with 6 classified as very sick. This study evaluated the effect of this illness on semen production. Semen output on 40,950 ejaculates from 583 Holstein bulls collected from July 1, 1975, through March 31, 1981, was analyzed to establish normal semen production and to estimate the effect of illness caused by Dursban 44 treatment. Ejaculate number, days between collections by previous number of ejaculates, calendar months, years, and ages of bulls affected the semen output characteristics, original volume, sperm concentration, percent motile sperm, total sperm per ejaculate, percent prefreeze discards, percent postthaw sperm motility, and percent postthaw discards. Ejaculate volume, motility, total percent prefreeze discards, and percent postthaw discards were influenced negatively on the 6 very sick bulls. Percent postthaw discards were higher on all bulls treated with Dursban 44 for up to 6 mo post-treatment.

Animals

Comparison of first lactations and all lactations of dams to predict sons' milk evaluations.

Multiple regression of genetic evaluations of about 170 Holstein bulls on sire, dam, and maternal grandsire genetic evaluations indicated that the partial regression coefficient for the sire is similar to the theoretical regression coefficient (.45 vs. .50), that the regression coefficient for the dam is much smaller than the theoretical regression coefficient when all records of the dam including her first are included in her evaluation (.12 vs. .35), although about as expected when only first records of the cow and her herdmates are used (.33 vs. .35), and that the regression for the maternal grandsire is larger than expected when his proof and all records of the dam are used in her evaluation (.07 vs. .00), although about as expected when his proof and only her first record is used (-.02 vs. .00). Preferential treatment of potential bull dams is a possible explanation for these results. Genetic correlations among lactations are not likely to be small enough to account for the differences in regression coefficients for the dam's genetic evaluations for all and first lactations. If conditions for selecting bull dams in the future will be similar to what they have been in the past, the conclusion is that genetic evaluations of cows from their first lactation records be used in preference to genetic evaluations from all lactation records for selection of dams of bulls.

Animals

The relationships between energy balance, milk production and ovulation in postpartum Holstein cows.

Energy balance and serum progesterone concentrations were monitored during early lactation in a group of high producing Holstein cows. The postpartum interval to normal ovulation averaged 36 +/- 6 days (range 6 to 83 days). Average energy balance during the first 20 days of lactation (cumulative daily calculated energy balance divided by days in milk) was inversely related to days to normal ovulation (r = -.60) and to milk production (r = -.80). Milk yield during this period was not closely related to days to ovulation (r = .30). Milk yield was also not significantly related to serum prolactin concentrations over the first 12 weeks of lactation (r = .27). During early lactation, energy balance was maximally negative until peak milk yield and then began returning toward zero, with the magnitude and duration of negative energy balance being quite variable. On the average, ovulation and the initiation of the first normal luteal phase occurred approximately 10 days after energy balance began returning toward zero. During this 10-day interval, a transient elevation in serum progesterone concentrations occurred in eight of 13 cows. These results suggest that energy balance during the first 20 days of lactation is important in determining the onset of ovarian activity following parturition.

Animals

Effect of adrenocorticotropin on milk and plasma cortisol and prolactin concentrations.

Milk cortisol and prolactin concentrations were measured in 12 lactating Holstein cows for 3 days (Experiment 1). On day 3, cows were divided randomly into groups: IV1, IV4, IM, and control. Group IV1 received one intravenous injection of 49 IU adrenocorticotropin, group IV4 the same dosage four times at 2-h intervals, group IM 240 IU adrenocorticotropin intramuscularly, and control cows were injected with saline. Cortisol concentrations in blood plasma were increased in all groups following adrenocorticotropin injection, while milk cortisol concentrations increased fourfold in groups IV4 and IM and remained unchanged in group IV1. In Experiment 2, infusion of 18% saline into the mammary gland of three cows increased the somatic cell count in the infused quarter but had no effect on prolactin and cortisol of milk or plasma. In Experiment 3, six cows each were assigned to treatment groups of saline controls, IV2-0 (40 IU adrenocorticotropin intravenously at 0 and 2 h post-milking) or IV2-8 (40 IU adrenocorticotropin intravenously at 8 and 10 h post-milking). Half udders of each cow were milked before treatment and 4 h later. Increases of cortisol in plasma increased cortisol concentrations of milk 4 h after each treatment (IV2-0 and IV2-8). However, by 12 h after treatment in IV2-0 cortisol concentrations of milk had returned to normal. Increases in adrenal cortisol secretion are followed rapidly (within 4 h) by increased cortisol concentrations in milk. However, these decline rapidly in the absence of sustained increments in blood cortisol. Increased cortisol concentrations in milk most likely represent sustained elevation in plasma cortisol.

Adrenocorticotropic Hormone

Sources of variation of semen output.

Data from 5033 ejaculates during 1 yr from 55 mature Holstein bulls were analyzed to determine the effects of ejaculate number, days between ejaculates, month of the year, solunar periods, and sires on spermatozoal output and seminal characteristics. First ejaculates contained 49.7% more sperm and 40.8% more sperm per ml than second ejaculates. Increasing the interval between collections from 2 to 6+ days resulted in 45.9% more sperm per ejaculate, 31.0% more sperm per ml. and 14.0% greater volume. However, daily sperm output was highest when bulls were ejaculated most frequently. Lowest semen output was in February and March, associated with a decrease in sperm per ml. Highest semen output was in April and June, associated with the highest concentration of sperm per ml. Solunar periods had no effect on any measure of semen output. The concentration of sperm per ml varied more than the volume per ejaculate and had the largest influence on variation in total sperm harvested per ejaculate. Frequency of ejaculation was a major factor in total sperm harvested per bull per year.

Animals

Effect of drying off practices on mastitis infection.

Two methods of drying off cows, intermittent milking and abrupt cessation, were studied with data from 9254 quarters of cows on 36 New York dairy farms. Eighty percent of the cows were infused with nine different antibiotic preparations separately at drying off, and 20% served as controls. Cows dried off by intermittent milking has a similar number of quarters infected at drying off, had fewer spontaneous recoveries, had a higher rate of cure, and developed fewer new infections in control quarters in comparison with cows dried off by the stop method. Methods worked equally well in treated cows. However, intermittent milking resulted in fewer infections at subsequent calving than stop milking in nondry treated cows. Cows producing less than or equal to 4 kg of milk at drying off were more highly infected than higher producing cows. Hind quarters contained more infections at drying off, fewer responded to therapy, and more infections developed in the dry period. Cows with dry periods of less than or equal to 30 days had more infected quarters respond to therapy and had the fewest new infections in the dry period. The role of routine dry cow therapy in decreasing the number of infections in dairy herds by preventing new infections and removing old infections is demonstrated.

Animals