A new automated uric acid assay.
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Biomedical subjects
Publications and source records attributed to R W Fong.
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1. The method of Parekh and Jung for determination of inorganic phosphorous in serum has been adapted for automation. One of the assets of the method is its unusually stable reagents. The automated procedure is highly accurate, sensitive, rapid and economic. Its other advantages are discussed in light of the literature.
Interference from various physiological and non-physiological steroids in the spectrophotometric determination of cholesterol by the Zak method (ferric chloride) and the method of Parekh and Jung (ferric acetate) was quantitatively measured. Contribution of the steroids at the specific absorption maxima of the cholesterol assays was determined by employing the steroids (40 mg/dl) alone, or added to a serum pool of known cholesterol content. The results show that the method of Parekh and Jung is less influenced by the presence of steroids than the Zak method. Observations on the structural specificity of the iron-cholesterol reaction are discussed.