ON THE CYTOLOGICAL UNIT FOR PROTEIN SYNTHESIS IN VIVO IN E. COLI. I. STUDIES WITH SPHEROPLASTS OF TYPE K-12.
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Biomedical subjects
Publications and source records attributed to R W HENDLER.
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A new and simple technique has been developed (10) for correcting C(14) radioactivity measurements for loss of radiation due to self-absorption. Results are discussed which show the applicability of the technique for many different counting situations. It is found that the absorption of radiation follows a hyperbolic law much more closely than an exponential one. Furthermore, the absorption coefficient for the sample itself has been found to be a function of weight rather than a constant, as had been assumed for the derivation of the law of exponential absorption.
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1. The electron and light microscope have been employed in a cytological study of the albumin-secreting cells of the hen oviduct and of fractions of this tissue obtained after homogenization and differential centrifugation. 2. These studies confirm the observation that in this tissue material corresponding to liver "microsomes" in amino acid-incorporating ability and ribonucleic acid content sediments in relatively low centrifugal fields. 3. The electron microscope studies suggest that the protein secretion of the gland is formed in intimate association with the ergastoplasm.
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