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Biomedical subjects

R W Lacey

Publications and source records attributed to R W Lacey.

At least 19 recordsLinked to original sources
PubMed1991

Evaluation of the Rosco system for the identification of Listeria species.

The Rosco system was used to identify previously confirmed isolates of the seven currently recognised species of Listeria. These included reference cultures and recent isolates from clinical material, food products and environmental sources. The system identified all correctly. Results were obtained after 4 h if heavy inocula, as suggested by the manufacturers, were used. The method may be used to aid identification of isolates of Listeria from clinical and non-clinical specimens and would be of particular value in laboratories examining small numbers of isolates relatively infrequently. Essential tests not included in the system are beta-haemolysis on sheep-blood agar and the CAMP test.

Listeria↗
PubMed1990

The efficacy of tobramycin in the treatment of ulcerative colitis.

This paper reports a double-blind placebo-controlled trial of oral tobramycin in acute ulcerative colitis. Eighty-four patients with an acute relapse of ulcerative colitis were randomized to receive oral tobramycin or placebo for 1 week as an adjunct to steroid therapy. At endpoint, 31 of 42 (74%) in the tobramycin group achieved complete symptomatic remission compared with 18 of 42 (43%) in the placebo group (P = 0.008). The tobramycin group achieved better histological scores (P less than 0.05) at endpoint. These findings show that treatment with oral tobramycin improves the short-term outcome of patients with ulcerative colitis in relapse.

Adult↗
PubMed1990

Evaluation of the Mast ID and API 50CH systems for identification of Listeria spp.

A multipoint inoculation technique (Mast ID) for the identification and species determination of Listeria monocytogenes (sensu strictu) and six other species of the genus Listeria was evaluated. This was compared with the commercially available API 50CH system. Both methods successfully identified all 123 strains tested. The Mast ID system is inexpensive and utilizing a multipoint inoculation technique permits the screening of up to 21 isolates per 9-cm petri dish. The API 50CH system was more expensive and time consuming and is therefore suitable only for the examination of smaller numbers of strains.

Bacteriological Techniques↗
PubMed1988

Rarity of tylosin resistance in human pathogenic bacteria.

Of 3812 human cultures of Staphylococcus aureus, Streptococcus pyogenes and Campylobacter species only 1.0 per cent were resistant to tylosin, an antibiotic used extensively in animals but not in man. There was no evidence for a significant animal source of these resistant cultures, a result which provides further evidence for the rarity of the flow of resistant organisms (or their genes) from animal sources to human beings.

Campylobacter↗
PubMed1988

Selection of resistance to gentamicin and netilmicin in the faecal flora following prophylaxis for colo-rectal surgery.

The selection of aminoglycoside-resistant bowel flora, following the administration of either gentamicin or netilmicin in combination with metronidazole for prophylaxis, during colo-rectal surgery in 88 patients has been examined. Both antibiotic regimens resulted in the selection of an aminoglycoside-resistant flora in a total of 57 (65%) of patients: in half of the patients there was a net gain in the aminoglycoside-resistant flora, and in 13 (15%) one aminoglycoside-resistant strain present prior to prophylaxis was displaced by another following operation. Three patients (3%) lost aminoglycoside-resistant strains after prophylaxis. Most of the resistant organisms selected were considered to be of little importance as potential pathogens, at least in the short term. In only a small minority (5%) of patients were aminoglycoside-resistant enterobacteria isolated. Aminoglycoside-resistant Staphylococcus aureus was not isolated. Of the resistant enterobacteria, only one strain, an isolate of Enterobacter cloacae selected in a patient receiving gentamicin, carried a resistance determinant which was self-transmissible to Escherichia coli.

Clinical Trials as Topic↗