Histamine skin prick testing: evaluation of various container materials for long-term storage of histamine solutions.
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Drug Packaging↗
Biomedical subjects
R Warlow
Publications and source records attributed to R Warlow.
Histamine sensitivity influences reactivity to allergens.
The ability to mount an IgE response to allergens is a prerequisite for the development of positive allergen skin tests, and this is reflected to some extent by the observation that allergen-responsive subjects tend to have higher total serum IgE concentrations. To determine whether histamine sensitivity also contributes to allergen responsiveness, 893 subjects in a rural community were prick tested with 14 allergens and tenfold dilutions of histamine phosphate beginning at 1 mg/ml-1. IgE was measured in a subset of 400 subjects. Three-way contingency table analysis confirmed previous reports of an association between allergen responsiveness and IgE (p less than 0.001) and also demonstrated that allergen responsiveness is associated with sensitivity to histamine (p less than 0.001). This association is independent of IgE so that the additive effect of IgE and histamine sensitivity allows more nearly accurate prediction of allergen responsiveness than either measurement alone.
Adolescent↗
Extractable nuclear antigen autoantibodies and their association with other autoantibodies and thymoma in myasthenia gravis.
The presence of autoantibodies to Extractable Nuclear Antigens (ENA) in myasthenia gravis (MG) was evaluated. These antibodies represent a subset of antinuclear antibodies (ANA), with ENA consisting of RNA and nucleoprotein. ENA autoantibodies had a prevalence of 44% in MG when tested by an
Adolescent↗
Angioimmunoblastic lymphadenopathy associated with large-muscle arteritis.
This is the reported case of angioimmunoblastic lymphadenopathy associated with large-muscle arteritis. Additional features included severe thrombocytopenia, IgG lambda paraproteinemia, and terminal staphylococcal and candidal infections.
Aged↗
An association between cutaneous sensitivity to histamine and HLA phenotype.
Four hundred and twenty randomly chosen subjects from a normal population were HLA typed and tested for cutaneous sensitivity to histamine by prick testing with 5 concentrations of histamine (10(-3), 10(-2), 10(-1), 1, 10 mg X ml-1). Positive responses to 10(-1) mg X ml-1 histamine occurred in 41% of the subjects, and particularly those with HLA-B7 (55%) (p less than 0.005). It is concluded that genes within the major histocompatibility complex influence cutaneous responses to histamine.
HLA Antigens↗
A rapid screening enzyme linked immunosorbent assay for autoantibodies to extractable nuclear antigens.
An enzyme linked immunosorbent assay (ELISA) has been developed to detect antinuclear antibodies to extractable nuclear antigens (ENA)--a rabbit thymus nuclear derivative. A second ELISA has also been developed (ENA RNA-ase ELISA) to analyse the relative contribution of the components of ENA (nucleoprotein and RNA antigens) to antibody binding as detected by the ELISA assays. Both ELISA assays provide a rapid screening method for antibodies to soluble, diffusable nuclear antigens that could otherwise go undetected by immunodiffusion or the indirect immunofluorescent method that uses unfixed frozen sections of rat liver. The considerable improvement that these ELISA assays offer in efficiency and both diagnostic and serological sensitivity over the traditional immunodiffusion method used for specificity characterisation of ENA autoantibodies is discussed.
Antibody Specificity↗
Tumour immunobiology.
A hypothesis for the interactional mechanism between tumour cells and the lymphopoeitic system, with its resulting sequence of immune depression is outlined, along with a review of the current literature relating to this area. The concept that the tumour cell surface is responsible not only for loss of cell surface contact inhibition, but also protecting itself from the lymphopoeitic elements that come into contact with it by infecting them with nucleic acid particles produced by its excessive nuclear turnover, is espoused, with a variety of supporting data.
Animals↗