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Biomedical subjects

R X Wang

Publications and source records attributed to R X Wang.

At least 37 records · Page 2Linked to original sources

Treatment of severe beta-thalassemia (patients) with myleran.

We previously reported that myleran, a cell cycle nonspecific drug, can stimulate gamma-globin gene expression in anemic adult rhesus monkeys. This finding prompted us to treat two patients with severe beta-thalassemia with myleran. Both patients received an initial course of therapy, constantly of myleran at a dosage of 0.2 mg/kg/d for 9 days followed by 0.15 mg/kg/d for the next 11 days. One patient received an additional 20-day course of myleran at a dosage of 0.2 mg/kg/d beginning 44 days after completion of the first course. No severe ill effects related to the drug were observed during or after drug administration. After 20 days of myleran treatment, levels of HbF and reticulocytes increased in both patients and level of F cells increased in patient 1. In patient 1, Hb concentration rose from 42g/L (9 days after transfusion) before treatment to a maximum of 65g/L afterward; in patient 2, it rose from 74g/L to a maximum of 106g/L. A value of 15g/L above baseline lasted for about 5 months in both patients. Hypomethylation of bone marrow DNA near the gamma-globin gene was demonstrated in patient 1.

Busulfan↗

Experiences in the treatment of electrical burns covering deep wounds with various tissue flaps.

From 1979 to 1986, 121 patients with electrical burns were admitted to our, unit (6% of all the burned patients admitted during that period). Of these cases 64 were regarded as having deep burns (e.g. burns which injured muscles, tendons, nerves, bones and capsules of the joints etc.). Thirty-five flaps of various types of tissues (including one free muscle flap, two island fascial flaps, four island skin flaps, three arterial skin flaps, two musculocutaneous flaps and twenty-three random skin flaps) were used for the coverage of deep burns in twenty-seven patients. Thirty three flaps survived completely while two were partially lost; three flaps developed severe sub-flap infections but the tissue of the flaps survived totally. Three typical cases are reported in this paper. The advantages of early extensive exploration and thorough debridement of the deep electrical wounds are discussed. It also introduces the practical usage of various flaps for wound coverage in these typical cases. When flaps were needed for deep electrical burns, we chose local flaps first, and used axial pattern flaps, island flaps, etc. as much as possible; if only a random flap was available in the site, we elevated it with some deep fascia to enhance its ability to prevent infection. We considered the free tissue transfer and the transfer of fascial flaps to be important alternatives. Debridement followed by immediate wound coverage should be done as soon after resuscitation as possible. Generally the operations were performed within the third to fifth days after the electrical burn. This is the best period for active treatment in order to salvage the remaining functions of the burned extremities. Operations performed after six days must be done only under the careful control of anti-infective measures. From our experience we have found that muscle flaps and musculocutaneous flaps were the most suitable approaches for the coverage of deeply infected wounds especially when bones were involved. Careful attention was taken to excise the necrotic muscle tissues, especially those that were "sandwiched" between necrotic muscle bundles. This was done primarily by gross evaluation according to the judgement of the surgeon intraoperatively. The swollen and hardened regions of the skin of involved extremities generally corresponded to the areas of necrotic muscle tissue at about three days after the injuries or later. This was used as a reference to indicate where to place or extend the exploratory incisions. We frequently preserved denatured peripheral nerves and tendons, because these injured structures often later regain their functions partially or completely with a reliable coverage.(ABSTRACT TRUNCATED AT 400 WORDS)

Accidents, Occupational↗

Restriction primer extension method of labeling oligonucleotide probes and its application to the detection of Hb E genes.

A new method for labeling oligonucleotides was developed to obtain high specific activity of radioactive probes. In an oligonucleotide molecule, two sequences were designed. One sequence, the 5', contains 19 nucleotides and serves as a template for probe synthesis. The second sequence, 3', contains a consensus sequence which forms a Pst I site after forming a complementary strand with the primer. In the presence of E. coli DNA polymerase I (Klenow fragment), alpha-32P dNTP and other dNTPs, a radioactive labeled oligonucleotide was synthesized by the primer extension method. After Pst I digestion, the probe was different from its template in length by 4 bp and could be separated from each other on urea-polyacrylamide gel electrophoresis (PAGE). A radioactive oligonucleotide probe with extremely high specific activity up to 10(10) dpm/micrograms could be obtained by the use of this method. The oligonucleotide probes have been used for the detection of the Hb E mutation in this report.

Base Sequence↗

Protein composition of the luminal fluid and protein synthesis in vitro by the oviducts and uteri of ovariectomized, pro-oestrus and 5-day pregnant rats.

Serum, oviducal and uterine luminal fluid proteins from rats at three different physiological stages of reproduction were analysed by polyacrylamide gel electrophoresis. Protein synthesis and secretion were also studied by short-term incubation of tissue pieces in vitro with radioactive leucine. In ovariectomized animals and 5-day pregnant animals, uterine weight and the protein content of the luminal fluid was significantly lower than in pro-oestrus animals. These differences were much less marked in the oviduct. The protein patterns of oviducal fluid and uterine fluid were significantly different from those of blood serum. However, certain proteins, such as those of 51, 56, 68 and 80 K are clearly derived from blood serum. The protein profile of oviducal fluid and extracts of oviducal tissue showed no obvious differences at the three physiological stages. However, some differences at the three physiological stages were evident in proteins of uterine fluid and in extracts of uterine tissue.

Animals↗

Alpha-1-antitrypsin types in five Chinese national minorities.

The frequencies of alpha-1-antitrypsin alleles were determined for five Chinese national minorities: Uigur, Korean, Mongolian, Chuang, and Li. PI S and PI Z alleles are not found in the five populations studied. PI ETOK allele is present in Korean, Mongolian, and Chuang populations, and Etokyo is a very common alpha-1-antitrypsin variant in Chinese. Other alleles which occur in more than one of the minorities are PI X (Mongolian and Chuang) and PI M4 (Korean and Uigur). The high frequency of PI M4 in the Uigur population suggests a probably Occidental origin of this nationality.

Alleles↗

Geographical variability of alpha-1-antitrypsin alleles in China: a study on six Chinese populations.

Alpha-1-antitrypsin phenotypes of six Chinese Han populations (20 degrees-45 degrees N. latitude) were determined. The frequency of allele M2 increases from North to South China. The north-south change of M2 appears to be mainly at the expense of alleles M1 and M3. Geographical variability of other variants was also observed. In the northern populations, the most common variants are M4 and Etokyo; in the southern populations, Pweishi, a variant which can not be distinguished electrophoretically from Pyasugi. These results form a distribution pattern of alpha-1-antitrypsin alleles in China.

Alleles↗