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Biomedical subjects

R de Vita

Publications and source records attributed to R de Vita.

6 recordsLinked to original sources
PubMed1994

Cellular effects of image diagnostic ultrasound on murine spermatogenesis monitored by flow cytometry.

A study has been carried out to evaluate the possible cellular effects induced by image diagnostic ultrasound on murine spermatogenetic cells. Exposure to ultrasound was carried out using a commercial diagnostic instrument that operates in B-mode. Male hybrid F1 mice, aged 8-10 weeks, were exposed to ultrasound for 30 min and observed from 7 to 35 days after treatment. Flow cytometric analysis has been used to monitor the relative frequency of the different types of spermatogenetic cells. This analytical approach showed changes in cell frequency in the compartment containing elongated spermatids which was used as an endpoint. A statistically significant decrease in the frequency of this cell type was observed 21, 28 and 35 days after exposure. These changes suggest that there may be a cytotoxic and/or cytostatic effect on spermatocytes and spermatogonia. These results showed that image diagnostic ultrasound induces effects on murine spermatogenesis at cellular level and that the flow cytometric approach makes it possible to identify quantitative cellular changes with reference to specific cell type.

Animals↗
PubMed1991

Cellular DNA content and proliferative activity evaluated by flow cytometry versus histopathological and staging classifications in human bladder tumors.

Flow cytometric analysis of cellular DNA content was performed on 78 biopsy bladder samples obtained from 61 patients with bladder tumors. All 6 normal tissue samples and 1 benign papilloma exhibited a cytometrically diploid DNA distribution, while 39 of 60 bladder carcinomas exhibited at least one aneuploid cell subpopulation. Furthermore, 13 of 39 aneuploid tumors were characterized by the presence of more than one aneuploid cell subpopulation. The results indicate a significant relationship between cytometric ploidy and morphological classification and stage: the occurrence of subpopulations with abnormal DNA content is associated with the increase in differentiation grade and stage. A significant statistical difference in the survival pattern between the diploid and aneuploid groups was observed. The percent of S cells extracted from DNA content distribution histograms indicates a statistically significant difference (p less than 0.01) between normal tissue (3.7 +/- 1.8), diploid tumor (8.4 +/- 3.9) and aneuploid tumor (14.9 +/- 6.0). Moreover the percent of S-phase cells increases with grade in only the aneuploid subgroup. Our results suggest that cytometric parameters in association with morphological and clinical criteria can contribute to a more accurate characterization of bladder tumors in prognostic terms.

Aged↗
PubMedDate not supplied

Children exposed to chronic contamination after the Chernobyl accident: cytogenetic and radiotoxicological analyses.

In this study, we describe cytogenetic studies of lymphocytes obtained from children who were exposed after the Chernobyl accident to low doses of ionizing radiation. We sought to determine possible chromosomal damage relative to internal contamination, as measured by whole-body counter and urine radiotoxicological analyses. The study was performed during a 1-mo period on the peripheral blood of children hosted in Italy, but who resided in contaminated regions of the Russian Federation and Belarus. We used conventional cytogenetics to detect chromosomal aberrations. In some cases, we also used "chromosome painting" to look for stable aberrations. There were more acentric fragments in subjects than in controls; a few chromosome and chromatid breaks werefound in the subjects, but this finding did not differ significantly between subjects and controls.

Body Burden↗
PubMedDate not supplied

Flow cytometry and sizing for routine andrological analysis.

Flow fluorometry and Coulter type sizing analysis of sperm have been applied separately in order to improve human semen analysis. Different methods of sample preparation were evaluated and a protocol involving prestaining pepsin treatment of sperm samples is proposed for fluorometric analysis. The data obtained with fluorometry and sizing analysis result in different kinds of information: Coulter counting allows to automate sperm counting and fluorometry yields more detailed information about normozoospermia and oligozoospermia by determining the proportion of mature spermatozoa and immature germ-cells. These two methods, together with light microscopy, may help to explore the correlation of fertility and pathology of spermatozoa. The aim of these investigations is to yield the preconditions for simultaneous two-parameter analysis of DNA content and cellular size distributions.

DNA↗
PubMedDate not supplied

[Nasal conduction and ventilation: study of 30 children with food allergy before and after adenoidectomy].

UNLABELLED: Observed the results of an investigation on the trouble of breathing in 1350 children, looked at the trouble's frequence (450 positive and 400 dubious), the Authors studied the obstruction of the high and peripheral, air-ways and the associated auditive troubles in relation with aerial allergens and foods allergens. METHODS: For the diagnosis of food's allergens: broncho-stimulation test by foods; rhinomanometry with Broms' formula, spirometry and Rresp exam, audiometric and impedentiometric exam. RESULTS: About 30 children with air-ways obstruction and positive at the bronchostimulation test to food's allergens 7 were Prick and Rast positive to aeroallergens'; 12 showed total obstruction of nasal pipes and audiometric and impedentiometric troubles, 15 obstruction of one only nasal pipe, 3 absence of obstruction. At the spirometric check and of Rresp in the 63.3% of children was associated obstruction of peripheral air-ways and in the 26.6% laryngeal obstruction. The only nasal obstruction was present in 10% of children, in the 48% was associated to conjunctivitis. In 12 children with audiometric and impedentiometric damage who were adenoidectomized two months after the surgical intervention the nasal condition showed evident improvement but in 5 children after one year the nasal obstruction showed itself again. The food allergens exclusion caused disappearance of nasal, laryngeal and peripheral obstructive syndrome and normalization of audiometric and impedentiometric values in the adenoidectomized subjects and in 95% of staying children.

Adenoidectomy↗