Biomedical subjects

Raisa M. Zorina

Publications and source records attributed to Raisa M. Zorina.

2 recordsLinked to original sources

PubMed2001

Alpha2-Macroglobulin Modulates Interactions between Lymphocytes and Fibroblasts.

The aim of the present study was to evaluate the influence of apha2-macroglobulin (alpha(2)M) on lymphocyte adhesion to fibroblasts. Peripheral blood lymphocytes from healthy donors and two fibroblast lines (human diploid embryo fibroblasts M-19 and mouse transformed fibroblasts L929) were used in the experiments. alpha(2)M treatment of fibroblast monolayer appeared to result in the enhancement of lymphocyte adhesion to fibroblasts. The number of attached lymphocytes was increased by 2-2.5 times. It should be noted that the effect of alpha(2)M didn't depend on the conformational molecule changes, since either native or methylamine or plasmin transformed alpha(2)M approximately at the same fashion increased the lymphocyte adhesion to both allogeneic and xenogeneic fibroblasts. B lymphocytes were predominant cells that were attached to fibroblast monolayer without alpha(2)M treatment. However the percentage of adherent T lymphocytes was increased substantially after the fibroblast monolayer treatment by alpha(2)M. Subpopulation analysis has shown that fibroblast pretreatment by alpha(2)M didn't result in a selective adhesion of CD4(+) or CD8(+) T lymphocytes, but increased the adhesiveness for both T lymphocyte subpopulations. The data obtained demonstrate that besides its participation in the processes of fibroblast adhesion alpha(2)M is capable to modify the contact interaction of these cells with lymphocytes that may have an influence on the functional consequences of this process.

Journal Article↗

PubMed2001

Pregnancy-Associated Plasma Protein-A, Alpha-2-Macroglobulin, Pregnancy Zone Protein and Their Complexes with IgG in Sera of Healthy Non-Pregnant and Pregnant Woman, and Patients with Breast Cancer.

The purpose of this study was to measure alpha-2-macroglobulin-IgG (alpha(2)-MG-IgG), pregnancy zone protein-IgG (PZP-IgG) and pregnancy-associated plasma protein-A-IgG (PAPP-A-IgG) complex concentrations in serum in comparison with total alpha(2)-MG, PZP and PAPP-A concentration, and to determine the possible role of such complexes. Serum samples were obtained from 20 healthy women, 30 patients with verified metastatic breast cancer (stage III-IV) before the treatment and 40 healthy women with normal pregnancy (I-III trimester in dynamics). The serum concentrations of alpha(2)-MG-IgG, PZP-IgG and PAPP-A-IgG complexes were measured by ELISA. It has been shown that the immune complexes of the major proteinase inhibitors such as alpha(2)-MG, PZP and PAPP-A with IgG may be found at minor concentration in blood of both the healthy women and patients with breast cancer. On the other hand, their physiological functions in organism are absolutely different. We suggest that alpha(2)-MG-IgG and PZP-IgG complexes can serve as immunoregulatory signal molecules. Thus, after calculation of a molar ratio, less than 1% of alpha(2)-MG and PZP were marked by IgG. On the contrary, one PAPP-A molecule can bind to 10-15 IgG molecules in samples of control non-pregnant women and breast cancer patients. Such a kind of binding gives an evidence in favor of alternative way of utilization and degradation of PAPP-A by monocyte-macrophages through a Fc-receptor.

Journal Article↗