Biomedical subjects

Ramakrishnan Parthasarathi

Publications and source records attributed to Ramakrishnan Parthasarathi.

6 recordsLinked to original sources

PubMed2006

Interaction of chromium(III) complex of chiral binaphthyl tetradentate ligand with DNA.

Since conformation of the molecule plays a vital role in the activity of drug, we have investigated the DNA interaction of a chromium(III) complex with ligands in two conformations. Chromium(III) complexes derived from chiral binaphthyl Schiff base ligands, viz. R- and S-2,2'-bis(salicylideneamino) 1,1'-binaphthyl, have been synthesized and characterized by mass, IR, and electronic spectra. The interaction of these R- and S-binaphthyl Schiff base chromium(III) complexes with CT-DNA was investigated with the goal of examining whether the chirality has an influence on the chromium(III)-DNA binding properties. The difference in chirality of the ligand did not show any striking difference in binding properties. The binding constants for R and S conformers were estimated to be 18 (+/-0.4) x 10(3) and 9.4 (+/-0.3) x 10(3) M(-1), respectively, through spectroscopic titrations. All the experimental results are suggestive that both the isomers are DNA groove binders. The results of steady-state as well as time-resolved fluorescence experiments, however, suggest that the R conformer has restricted mobility when bound to DNA because it is more deeply buried in the groove of DNA compared to the S isomer.

Chromium↗

PubMed2006

Minimally invasive esophagectomy: thoracoscopic mobilization of the esophagus and mediastinal lymphadenectomy in prone position--experience of 130 patients.

BACKGROUND: To evaluate outcomes after minimally invasive or thoracolaparoscopic esophagectomy (TLE) with thoracoscopic mobilization of the esophagus and mediastinal esophagectomy in prone position. Esophagectomies are being performed increasingly by a minimally invasive route with decreased morbidity and shorter hospital stay compared with conventional esophagectomy. Most series report thoracoscopic mobilization of the esophagus and mediastinal lymphadenectomy in the left lateral position with respiratory complications up to 8% and prolonged operative time, probably because of inadequate stance of the surgeon during the thoracoscopic part. This study shows the potential of the thoracoscopic part of the procedure in prone position to ease these difficulties. STUDY DESIGN: From January 1997 through April 2005, TLE was performed in 130 patients. All patients had histologically proved squamous cell carcinoma of the middle third of the esophagus. Only one (0.77%) patient received neoadjuvant chemotherapy. The thoracoscopic part of the procedure was performed in prone position with excellent ergonomics, translating into less operative time and better respiratory results. We performed a minilaparotomy to retrieve the specimen owing to bulky tumors. Feeding jejunostomy and pyloromyotomy were performed in all patients. RESULTS: There were 102 men and 28 women. Median age was 67.5 years (range 38 to 78 years). There was no conversion to open method. Median ICU stay was 1 day (range 1 to 32 days) and median hospital stay was 8 days (range 4 to 68 days). Perioperative mortality was 1.54% (n = 2). Anastomotic leak rate was 2.31% (n = 3). There was no incidence of tracheal or lung injury and a very low incidence of postoperative pneumonia. At mean followup of 20 months (range 2 to 70 months), stage-specific survival was similar to open and other minimally invasive series. CONCLUSIONS: TLE with thoracoscopic part in prone position is technically feasible, with a low incidence of respiratory complications and less operative time required. It provides comparable outcomes with other techniques of minimally invasive esophagectomy and most open series. In our experience, we observed a low mortality rate (1.54%), hospital stay of 8 days, and low incidence of postoperative pneumonia. It has the potential to replace conventional and other techniques of minimally invasive esophagectomy.

Adult↗

PubMed2005

Laparoscopic repair of high rectovaginal fistula: is it technically feasible?

BACKGROUND: Rectovaginal fistula (RVF) is an epithelium-lined communication between the rectum and vagina. Most RVFs are acquired, the most common cause being obstetric trauma. Most of the high RVFs are repaired by conventional open surgery. Laparoscopic repair of RVF is rare and so far only one report is available in the literature. METHODS: We present a case of high RVF repaired by laparoscopy. 56-year-old female who had a high RVF following laparoscopic assisted vaginal hysterectomy was successfully operated laparoscopically. Here we describe the operative technique and briefly review the literature. RESULTS: The postoperative period of the patient was uneventful and after a follow up of 6 months no recurrence was found. CONCLUSION: Laparoscopic repair of high RVF is feasible in selected patients but would require proper identification of tissue planes and good laparoscopic suturing technique.

Female↗

PubMed2003

Abdominal cocoon: sonographic features.

An abdominal cocoon is a rare condition in which the small bowel is encased in a membrane. The diagnosis is usually established at surgery. Here we describe the sonographic features of this condition.

Adolescent↗

PubMedDate not supplied

Laparoscopic appendectomy for mucocele of the appendix: Report of 8 cases.

Mucocele of the appendix is an aseptic dilatation secondary to obstruction. Surgical excision is the treatment of choice in benign mucocele. The incidence of mucocele of the appendix in our center is 0.15%, of a total of 6000 appendectomies over 8 years. We operated on 9 cases; laparoscopic appendectomy was done in 8 of them. One patient had pseudomyxoma peritonei, so open surgery was done. Other organs were also examined as there is a possibility of concurrent tumors. As there is risk of malignancy of the appendix leading to port-site metastasis we used a non-permeable bag to remove the resected specimen.

Appendectomy↗

PubMedDate not supplied

Laparoscopic appendectomy in pregnancy: a case series of seven patients.

BACKGROUND: Appendicitis in the pregnant patient presents with diagnostic difficulties and more serious problems. Open surgery has its own limitations, mostly governed by the stage of the disease and the trimester of pregnancy. Laparoscopic appendectomy is beginning to be recognized as standard appendicitis treatment, as evidenced by several studies. In pregnancy, laparoscopy is even more controversial. Several studies prove the safety of laparoscopy and some studies disprove it. METHODS: We have treated 7 patients in the last 10 years; 6 had acute appendicitis and 1 had a perforated appendix. Ultrasound diagnosed 5 patients, and CT scan identified the other 2 patients. The patient with the perforated appendix had free fluid in the right iliac fossa and pelvis. Laparoscopic appendectomy was done in all patients. DISCUSSION: Pregnancy poses its own unique problems to the surgeon and anesthesiologist. The normal physiology becomes altered, and sound knowledge of this is necessary to handle these patients. Clinical presentation was mostly straightforward. Both ultrasound and CT scan were useful investigations. CONCLUSION: Most studies support the use of laparoscopy in the management of appendicitis. There was no mortality (for mother and fetus) or morbidity in our patients.

Adult↗