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Ravi Mehrotra

Publications and source records attributed to Ravi Mehrotra.

23 records · Page 2Linked to original sources
PubMed2002

Comparison of in-house polymerase chain reaction method with the Roche Amplicor technique for detection of Mycobacterium tuberculosis in cytological specimens.

Two techniques have been approved by the United States FDA for diagnosis of tuberculosis in smear positive sputa: LCX M. tuberculosis, a ligase chain reaction procedure manufactured by Abbott Laboratories, and Amplicor, a polymerase chain reaction (PCR) procedure manufactured by Roche. However, these commercial methods are expensive and beyond the reach of laboratories in most developing countries. We compared the Roche Amplicor kit with an in-house PCR using a primer set for Mycobacterium tuberculosis/bovis directed at MPB 64 protein gene. It was able to distinguish between M. tuberculosis, M. avium, M. gordonii, M. intracellularae, and M. kansasii. Fifty-seven cytological samples were submitted to the laboratory for molecular diagnosis of M. tuberculosis. Both procedures were run on every sample submitted and the two methods agreed completely. The custom-made method is less expensive than the commercial technique.

Cytodiagnosis↗
PubMedDate not supplied

Trends of prevalence and pathological spectrum of head and neck cancers in North India.

BACKGROUND: Head and neck neoplasia constitute one of the commonest cancers in India. Use of smokeless tobacco (Pan masala, Zarda etc.) is on the increase in North India, especially in Uttar Pradesh, and is responsible for the large majority of these tumours. AIM: To assess the patients' characteristics, yearly prevalence and histopathological subtypes of the head and neck neoplasia (excluding oral cavity) in Allahabad and surrounding regions. SETTINGS AND DESIGN: A retrospective study of 11 years from 1990 to 2000 was designed. Data was collected year-wise using the tumor registry data. MATERIAL AND METHODS: All biopsies submitted for histopathology to the Pathology department were reviewed and analyzed for demographic data, site and diagnosis. STATISTICAL ANALYSIS: The Kolmogorov-Smirnov Two-Sample Test was utilized to determine whether two distributions are the same. RESULTS: A total of 40559 biopsies were examined in the department, of which, lesions of the head and neck region, excluding the oral cavity, constituted 694 biopsies (409 males and 285 females). One hundred and forty-four malignant lesions were reported, 114 being males and 30 females. A comparison of the age-specific prevalence rates of cancer during the study period showed that the prevalence was highest in patients belonging to the 50-59 years age group and squamous cell carcinoma Grade II was the most prevalent type. On an average, 58 new biopsies per annum were received. CONCLUSIONS: Properly structured site-specific data like this can augment the National Cancer Registry Programme and is an essential indicator for the magnitude and the pattern of the cancer problem in India.

Adolescent↗
PubMedDate not supplied

Use of complementary and alternative medicine by patients with diabetes mellitus.

BACKGROUND: A wide variety of alternative medicines have been traditionally used for the treatment of diabetes in India. We did a cross-sectional study to assess the use of complementary and alternative medicine by patients with diabetes attending our outpatient department. METHODS: Four hundred and ninety-three patients attending the outpatient endocrine clinic for allopathic treatment were included. They were interviewed to assess their knowledge, awareness and methods of practice of non-allopathic forms of therapy. Information on the patients' background characteristics, family history of disease, existing knowledge of their disease and therapy was obtained. RESULTS: The user rate of complementary and alternative medicine was 67.8% and this was not significantly associated with the educational or socioeconomic status of the patients. Desire for early and maximum benefit was the most common reason (86.8%) for using these remedies. The patients felt that acupressure followed by naturopathy were the most beneficial alternative therapies, while homeopathy was felt to have the least benefit in the control of diabetes. CONCLUSION: There was widespread use of complementary and alternative systems of medicine by our patients. It is therefore necessary to obtain objective data to assess the improvement in blood sugar level with, and side-effects of, these methods of treatment.

Adult↗
PubMedDate not supplied

Expression of p53, cyclin D1 and Ki-67 in pre-malignant and malignant oral lesions: association with clinicopathological parameters.

In this study, a possible association was examined between the immunoexpressions of p53, cyclin D1, Ki-67 and tobacco exposure and the risk of oral cancer (OC) in premalignant and malignant formalin-fixed, paraffin-embedded oral mucosal tissue specimens from patients from Yemen (n=24, all were pre-malignant) and India (n=16, 11 were OCs). Overexpressions of p53, cyclin D1 and Ki-67 were found in 100%, 45.5% and 80% of the OCs, compared to 65.5%, 82.8% and 85.1% of the pre-malignant lesions, respectively. In the pre-malignant lesions, a statistically significant correlation was found between histopathological grading and expressions of cyclin D1 (p = 0.001) and Ki-67 (p = 0.03), and between anatomical site and expression of Ki-67 (p = 0.01). Coexpressions of the three proteins in the cases examined was found to correlate significantly to each other (cyclin D1: p53, r = 0.48, p = 0.002; p53: Ki-67, r = 0.41, p = 0.008) except for cyclin D1: Ki-67. These findings suggest that the expressions of p53, cyclin D1 and Ki-67 might contribute to OC susceptibility in oral mucosal lesions examined from Yemen and India. The importance of the three proteins examined as biomarkers in OC and pre-malignant lesions deserves particular attention because it might offer further understanding of the development of these lesions, particularly in populations heavily exposed to tobacco habits. Abnormalities of both cyclin D1 and Ki-67 might play an important role in the development of oral pre-malignant lesions and warrant further studies. Larger studies are, therefore, necessary in the two countries to examine the role of these biomarkers in OCs and premalignant oral mucosal lesions.

Adult↗