Biomedical subjects

Renata Jacewicz

Publications and source records attributed to Renata Jacewicz.

8 recordsLinked to original sources

PubMedDate not supplied

[Population genetics of the D1S80 system in a Central Poland region].

Population data concerning the D1S80 system based on the analysis of 775 persons from Central Poland region was presented. Accordance with the Hardy and Weinberg equilibrium (HWE) was proved using two different statistical approaches. The parameters for the appropriateness of this marker for investigations in the analysed population was also assessed: heterozygosity--0.79, PIC--0.77, PD--0.93, PE--0.62, PI--12.8 and frequency of mutation events--0.27%.

Alleles↗

PubMedDate not supplied

[Evolution of Supreme Court settlements relating to DNA evidence in paternity investigations].

The evolution of Supreme Court settlements relating to DNA evidence in paternity investigations was presented in this paper. It was found that its opinions have essentially changed during the last years as follows: 1) superiority of DNA evidence over other proof was perceived by the possibility of positive paternity confirmation, 2) attention has been turned to advisability of statistical analysis and its possible positive influence on emotional ties between parents and child, 3) opinion has been expressed about crossing of borders of free judicial estimations in cases of questioning DNA expertise, 4) attention has been turned to the possibility of utilisation of other tissues than blood or indirect genotyping.

Child↗

PubMedDate not supplied

[Allele distribution of the ten STR loci in Central Poland population].

Allele frequency data for ten STR loci: D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA were estimated in a sample of 350 unrelated individuals in Central Poland population (Lodz region). The accordance with the Hardy and Weinberg equilibrium (HWE) for all investigated loci was proved. The allele distributions were compared to the similar data for other populations.

Alleles↗

PubMedDate not supplied

[STR and RFLP polymorphism in paternity determination based on family investigation].

This paper shows a paternity examination of a deceased putative father carried out on the basis of his family investigation. Statistical evaluation proved the defendant's paternity using STR and RFLP analyses with the probability of more than 99,999% (PI > 100 000). The restriction analysis has proven considerably more informative than the amplification analysis, which means seven RFLP markers correspond with the similar paternity indices of the fifteen STR markers.

DNA Fingerprinting↗

PubMedDate not supplied

[Frequency quotient of paternity index Qf in a case of replacement of the natural father within a trio with his brother].

Cases of trios in which the natural father's near blood-relative instead of the natural father are among the hardest to give an expert opinion on. The goal of this study was to determine with what frequencies specific given values of paternity index appear in trios with the natural father and with the natural father's brother, and whether these frequencies can be the basis for discovering the substitution of the natural father by a brother in a given trio examined. The study material was the population of genotypes investigated in the AmpF/STR SGM Plus system. It was seen that the higher the paternity index values, the more frequently they appear in trios with the natural father, and less frequently in trios with the natural father's brother. This dependency has a general character and obtained in all the systems studied. The frequency quotients Qf (defined as the frequency of a given PI value in trios with the natural father in relation to the "reduced" frequency of the PI value in trios with the brother of the natural father) are found in the region of c. 0.6 to c. 1.4, whereby extreme values are rare, and the most frequent are cases where Qf oscillates within modest limits around the value 1. This fact shows that the majority of cases, calculated Qf values contribute little to resolving the question of whether the defendant--or his brother--is the child's father.

DNA Fingerprinting↗

PubMedDate not supplied

[Practical usefulness of the IDENTIFILER system for paternity testing in the Central Poland population].

The usefulness of the IDENTIFILER multiplex system for paternity testing in the Central Poland population was examined. One hundred excluding cases and one hundred including cases were analysed and the results were estimated for two different types of cases: trios (standard cases) and duos (motherless cases). Efficiency of exclusion and paternity index were analysed for each locus as well as for the entire set of the fifteen STR markers.

DNA Fingerprinting↗

PubMedDate not supplied

[A case of mutation at locus TH01--sequence analysis].

In a paternity test with 21 short tandem repeats (STRs) an isolated exclusion for the TH01 locus was observed. The probability of paternity or maternity in this case turned out greater than 99,999%. The analysis of sequence indicates only the deletion character of the observed mutation event without any changes in the conservative sequence.

Base Sequence↗

PubMedDate not supplied

[Estimating the efficiency of DNA isolation methods in semen, blood and saliva stains using the QuantiBlot system].

The aim of this study was to compare and select the optimal method of DNA isolation from blood, semen and saliva stains, as well as to determine appropriate conditions for employing amplification kits for identification of individual persons [brak w polskim tekście]. The materials analyzed in this study consisted of stains of blood, semen and saliva samples stored for a year, and stains of blood stored for a month. Seven various methods of isolation were compared: the Fast DNA kit (Qbiogene), phenol/chloroform extraction, Sherlock (DNA II Gdansk), Dneasy (Qiagen), Wizard Genomic Purification Kit (Promega), Chelex 100 (Biorad) and salting out proteins method. After the isolation, the quantity of DNA was measured with QuantiBlot [brak w polskim tekście]. The highest DNA concentration in bloodstains stored for one year and one month was observed employing the salting out proteins method. The phenol-chloroform extraction method was also found to produce reasonably good results. Isolation from blood and semen with salting-out method appeared to be the most effective. The phenol/chloroform method was dependent on the age and origin of the materials [brak w polskim tekście]. The Sherlock kit was proven to be effective in blood samples stored for one year. DNA concentration values obtained in semen and saliva samples were very low and characterized by a low repeatability.

Analysis of Variance↗