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Biomedical subjects

Robert D Hoppa

Publications and source records attributed to Robert D Hoppa.

5 recordsLinked to original sources

Detection of a single nucleotide polymorphism in the IL-6 promoter region of ancient nuclear DNA.

In the current study a method was developed to examine the G/C single nucleotide polymorphism (SNP) at position -174 in the IL-6 promoter from nuclear DNA samples isolated from human skeletal remains from Manitoba, Canada, dating to as early as 3500 years ago. The IL-6 (-174) SNP was detected in three ancient samples and determined, as expected, in three out of three to be homozygous G/G. The analysis of cytokine SNPs of ancient nuclear DNA may provide novel insights into the genetic basis of autoimmune diseases and the susceptibility/resistance to infectious agents.

Anthropology↗

A test of sex determination from measurements of chest radiographs.

Sexually dimorphic distinctions within the human thoracic area may include morphological as well as metric differences in the sternum and 4th rib. This research assesses the validity of a set of previously published measurements from chest radiographs and their use in contemporary forensic situations. The chest plates from 130 adult individuals of a known sample undergoing medico-legal post-mortem examination were examined at autopsy. Thoracic radiographs were taken using a Faxitron cabinet X-ray machine at 40 kV using Kodak Diagnostic Film Ready Pack X-Omat TL. Measurements were taken to the nearest millimetre using a sliding calliper. Logistic regression analysis of measurements of the sternum and 4th rib was undertaken to determine sex. Using 4th rib width and sternal area, sex was predicted at an accuracy of 95.8% for males and 90.3% for females.

Adolescent↗

Assessing age-related morphology of the pubic symphysis from digital images versus direct observation.

The increasingly global role of a forensic anthropologist necessitates a proper means for archiving evidence for re-examination. Large quantities of evidence can be stored and be made readily accessible through digital imaging. This study focuses on age assessment from digital photographs for personal identity reconstructions. A comparison of 52 Suchey-Brooks scores assigned to digital images and actual bone revealed that age assessment from digital images can be completed with accuracy. Coefficients of concordance imply that there significant agreement between osteological assessment of aging criteria from digital images and direct observation-greater than random change alone (p < 0.05). However, assessments from images should be approached with caution since there are inherent limitations of the naked eye in identifying morphological changes in certain skeletal features, especially where older adults are concerned. Although there is no replacement for a hands-on physical assessment, a digital archive may facilitate the global needs of the forensic anthropologist.

Age Determination by Skeleton↗

Geographic analysis of diabetes prevalence in an urban area.

The objective of this research is to identify the sociodemographic, environmental, and lifestyle factors associated with the geographic variability of Diabetes Mellitus (DM) prevalence in the City of Winnipeg, Manitoba in Canada. An ecological regression study design was employed for this purpose. The study population included all prevalent cases of DM in 1998 for Winnipeg. Predictor and outcome data were aggregated for analysis using two methods. First, the spatial scan statistic was used to aggregate study data into highly probable diabetes prevalence clusters. Secondly, predictor and outcome data were aggregated to existing administrative health areas. Analysis of variance and spatial and non-spatial linear regression techniques were used to explore the relationship between predictor and outcome variables. The results of the two methods of data aggregation on regression results were compared. Mapping and statistical analysis revealed substantial clustering and small-area variations in the prevalence of DM in the City of Winnipeg. The observed variations were associated with variations in socioeconomic, environmental and lifestyle characteristics of the population. The two methods of data aggregation used in the study generated very similar results in terms of identifying the geographic location of DM clusters and of the population characteristics ecologically correlated to those clusters. High rates of DM prevalence are strongly correlated with indicators of low socioeconomic status, poor environmental quality and poor lifestyles. This analysis further illustrates what a useful tool the spatial scan statistic can be when used in conjunction with ecological regression to explore the etiology of chronic disease.

Analysis of Variance↗

Semiparametric method for estimating paleodemographic profiles from age indicator data.

This paper addresses the problem of estimating an age-at-death distribution or paleodemographic profile from osteological data. It is demonstrated that the classical two-stage procedure whereby one first constructs estimates of age-at-death of individual skeletons and then uses these age estimates to obtain a paleodemographic profile is not a correct approach. This is a consequence of Bayes' theorem. Instead, we demonstrate a valid approach that proceeds from the opposite starting point: given skeletal age-at-death, one first estimates the probability of assigning the skeleton into a specific osteological age-indicator stage. We show that this leads to a statistically valid method for obtaining a paleodemographic profile, and moreover, that valid individual age estimation itself requires a demographic profile and therefore is done subsequent to its construction. Individual age estimation thus becomes the last rather than the first step in the estimation procedure. A central concept of our statistical approach is that of a weight function. A weight function is associated with each osteological age-indicator stage or category, and provides the probability that a specific age indicator stage is observed, given age-at-death of the individual. We recommend that weight functions be estimated nonparametrically from a reference data set. In their entirety, the weight functions characterize the relevant stochastic properties of a chosen age indicator. For actual estimation of the paleodemographic profile, a parametric age distribution in the target sample is assumed. The maximum likelihood method is used to identify the unknown parameters of this distribution. As some components are estimated nonparametrically, one then has a semiparametric model. We show how to obtain valid estimates of individual age-at-death, confidence regions, and goodness-of-fit tests. The methods are illustrated with both real and simulated data.

Adolescent↗