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Biomedical subjects

Robert E Hausman

Publications and source records attributed to Robert E Hausman.

3 recordsLinked to original sources

Liposuction breast reduction: a prospective trial in African American women.

BACKGROUND: Recently published case reports and outcome studies support the use of liposuction alone as an effective technique for ameliorating symptoms of breast hypertrophy. This study is the first prospective trial to examine the effectiveness of liposuction breast reduction as a primary modality of breast reduction. In addition, this study examines the role that liposuction breast reduction can play in the treatment of African American women, given the known scarring difficulties that darker skinned patients can encounter with traditional breast reduction surgery. METHODS: Twenty African American women were recruited through newspaper and Internet advertisements. Patients aged 20 to 60 years were serially accepted to the study. Patients with a chief complaint of breast ptosis were excluded. No other exclusion criteria were used. Previously validated questionnaire instruments were used preoperatively and postoperatively to measure breast-related symptoms, general patient health perception, bodily pain, and self-esteem. Comorbid conditions, demographics, financial status, prior treatments, and smoking history were also documented. RESULTS: Seventeen patients completed the preoperative and postoperative questionnaires. An average of 1075 cc of tissue was removed per breast during liposuction breast reduction surgery. Postoperative assessment showed a significant decrease in breast-related symptoms, a significant decrease in patient pain, and a significant improvement in overall patient health perception. CONCLUSIONS: Liposuction breast reduction is a useful breast reduction modality in the properly selected patient. African American women, who may traditionally forego breast reduction surgery because of scarring, are excellent candidates for this type of reduction procedure.

Adult↗

Ocular extracellular matrices in development.

The extracellular matrix is known to play important roles in regulating tissue development and cell differentiation. At a basic level the matrix is the substratum to which cells adhere and over which they migrate during morphogenesis. The recognition and adhesion of cells to matrices, especially to specialized matrices such as basal laminae, in developing tissues plays an important role in their decision to adhere or move on, to continue mitosis, to differentiate or to undergo apoptosis. The matrix is also the milieu from which growth factors and morphogens interact with the cell. Recent work demonstrates that matrix molecules are often active participants in these signaling processes, regulating the cellular response. Our understanding of the complexity of different ocular matrices continues to grow with the characterization of new proteins that modify collagen fibrils or serve as ligands for cell surface adhesion receptors. This review looks at those aspects of development in major tissues of the vertebrate eye where the extracellular matrix is known to participate, or is likely to do so based on similar roles in the development of the embryonic forebrain or other tissues. It concludes by highlighting some of the major developmental questions about the roles of the matrix in development in several ocular tissues.

Animals↗

Cloning of chicken choline acetyltransferase and its expression in early embryonic retina.

The enzyme choline acetyltransferase [EC 2.3.1.6] (ChAT) synthesizes the neurotransmitter acetylcholine that plays a key morphogenic role in vertebrate retina development. As the embryonic avian retina is particularly useful for morphogenetic studies, we cloned the complete coding region of chicken ChAT cDNA. At the deduced amino acid level, chicken ChAT is approximately 76% identical to mammalian ChAT proteins. We also report here the cloning of the complete 5' end of the complex cholinergic locus. This locus contains both the ChAT gene and the nested intronless gene for the vesicular acetylcholine transporter (VAChT). The genomic organization of the 5' end of the chicken cholinergic locus is similar to that reported in other vertebrate species. A 5.7 kb mRNA corresponding to the ChAT message was detected in both embryonic retina and post-hatch brain. An analysis of the ChAT mRNA in embryonic chick retina shows that the message can be detected by E6 and its level increased during early retinal development. Vertebrate ChAT mRNAs can contain one or more of three non-coding exons, M, N or R and by RT-PCR we demonstrate, at least, a chicken ChAT mRNA containing exon M.

Amino Acid Sequence↗