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Robert Nowak

Publications and source records attributed to Robert Nowak.

3 recordsLinked to original sources

Robust contour matching via the order-preserving assignment problem.

A common approach to determining corresponding points on two shapes is to compute the cost of each possible pairing of points and solve the assignment problem (weighted bipartite matching) for the resulting cost matrix. We consider the problem of solving for point correspondences when the shapes of interest are each defined by a single, closed contour. A modification of the standard assignment problem is proposed whereby the correspondences are required to preserve the ordering of the points induced from the shapes' contours. Enforcement of this constraint leads to significantly improved correspondences. Robustness with respect to outliers and shape irregularity is obtained by required only a fraction of feature points to be matched. Furthermore, the minimum matching size may be specified in advance. We present efficient dynamic programming algorithms to solve the proposed optimization problem. Experiments on the Brown and MPEG-7 shape databases demonstrate the effectiveness of the proposed method relative to the standard assignment problem.

Algorithms↗

The effect of new non-cross resistant antitumour agents on the energy state of human erythrocytes.

Multidrug resistance (MDR) of tumour cells is related to the overexpression of ATP-dependent pumps responsible for the active efflux of antitumour agents out of resistant cells. Benzoperimidine and anthrapyridone compounds exhibit comparable cytotoxic activity against sensitive and MDR tumour cells. They diffuse extremely rapidly across the plasma membrane and render the ATP-dependent efflux inefficient. Such uptake could disturb an energy metabolism of normal cells possessing an elevated level of ATP-dependent proteins, especially erythrocytes having a high level of the MRP1, MRP4 and MRP5 proteins. In this study the effect of five antitumour agents: benzoperimidine (BP1), anthrapyridones (CO1, CO7) and reference drugs used in the clinic: doxorubicin (DOX) and pirarubicin (PIRA), on the energetic state in human erythrocytes has been examined. These compounds have various types of structure and kinetics of cellular uptake (slow--DOX, CO7, moderate--PIRA, fast--BP1, CO1) resulting in their different ability to saturate ATP-dependent transporters. The energetic state of erythrocytes was examined by determination of purine nucleotide contents (ATP, ADP, AMP), NAD(+) and values of adenylate energy charge (AEC) using an HPLC method. It was found that the level of nucleotides as well as the AEC value of erythrocytes were not changed during 24 h of incubation with these agents independently of their structure and ability to saturate ATP-dependent pumps. This is a very promising result in view of their potential use in the clinic as antitumour drugs against multidrug resistant cancers.

Antineoplastic Agents↗