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Roger Marrannes

Publications and source records attributed to Roger Marrannes.

2 recordsLinked to original sources

Identifying modulators of hERG channel activity using the PatchXpress planar patch clamp.

The authors used the PatchXpress 7000A system to measure compound activity at the hERG channel using procedures that mimicked the "gold-standard" conventional whole-cell patch clamp. A set of 70 compounds, including hERG antagonists with potencies spanning 3 orders of magnitude, were tested on hERG302-HEK cells using protocols aimed at either identifying compound activity at a single concentration or obtaining compound potency from a cumulative concentration dependence paradigm. After exposure to compounds and subsequent washout of the wells to determine reversibility of the block, blockade by a reference compound served as a quality control. Electrical parameters and voltage dependence were similar to those obtained using a conventional whole-cell patch clamp. Rank order of compound potency was also comparable to that determined by conventional methods. One exception was flunarizine, a particularly lipophilic compound. The PatchXpress accurately identified the activity of 29 moderately potent antagonists, which only weakly displace radiolabeled astemizole and are false negatives in the binding assay. Finally, no false hits were observed from a collection of relatively inactive compounds. High-quality data acquisition by PatchXpress should help accelerate secondary screening for ion channel modulators and the drug discovery process.

Cell Line↗

Computer programs to facilitate the estimation of time-dependent drug effects on ion channels.

The programs I-VOC and I-ROC have been designed to facilitate the analysis of drug effects on currents through ion channels in the cell membrane, measured by means of voltage-clamp. They are written in Igor Pro (WaveMetrics) and read exported files or raw data files generated by the Pulse (HEKA Electronic) or pClamp (Axon Instruments) programs. With I-VOC, the sweeps of current through voltage-operated ion channels can be quantified within six time ranges, and current run-down can be corrected for after the currents are fitted during the control period. Linear leak current can be subtracted even when the P/n method is not used. The results are plotted and tabulated. With I-ROC, an analogous program, receptor-operated currents can be quantified, the peak current or rate of desensitization can be fitted and run-down corrected for. Chart-like data can be converted to a sweep-like format. Several procedures are incorporated for rapid graphical data presentation. These programs accelerate and improve the estimation of drug effects on ion channels.

Animals↗