Biomedical subjects

Rong Lin

Publications and source records attributed to Rong Lin.

23 records · Page 2Linked to original sources

PubMed2003

[Detection of platelet activation by flow cytometry in patients with pre-eclampsia].

OBJECTIVE: To explore the clinical significance of expression alterations of static and activated platelet glycoprotein CD41a, CD62p, and CD63 in patients with pre-eclampsia. METHODS: We used non-washing immunolabeling technique for fluorescence labeling of the static and activated platelet with CD41a, CD62p, and CD63 monoclonal antibodies of the blood samples from 16 patients with pre-eclampsia, 15 women with normal pregnancies and 20 healthy subjects. The expression of the monoclonal antibody was detected by flow cytometry. RESULTS: The average static and activated platelet glycoprotein CD41a, CD62p, and CD63 expression levels in patients with pre-eclampsia were apparently higher than those of the normal pregnant women (P<0.01), while the comparison between the latter and the control group did not reveal any significant differences (P>0.05). CONCLUSION: High levels of platelet glycoprotein CD62p and CD63 expressions are present in patients with preeclampsia, suggesting the presence of platelet activation in these patients.

Adult↗

PubMed2003

[Analysis of alkaline phosphatase staining results in 238 cases of malignant lymphoma].

OBJECTIVE: To examine the significance of alkaline phosphatase (ALP) staining in clinical characterization of malignant lymphoma cases. METHODS: Fresh peripheral blood smear obtained from 238 cases of malignant lymphoma, including 23 cases with bone marrow involvement (BMI) 189 cases without BMI(NBMI), and 26 cases of lymphoma cell leukemia (LCL), were examined by ALP staining, and the results were compared with those of 40 normal control subjects. RESULTS: With the gradual progression of lymphoma, the patients tended to have higher ALP scores, and the percentages of the subjects with a score higher than 250 in the control, NBMI, BMI and LCL were 0, 4.8%, 39.1%, 53.9% respectively, showing significant differences between the four groups. CONCLUSIONS: Increased ALP activity can be indirectly indicative of lymphoma progression, and ALP staining results may provide insight into the clinical staging and prognosis of malignant lymphoma.

Adolescent↗

PubMed2003

[Surface morphology of CD34+ cells under atomic force microscope].

OBJECTIVE: To observe the difference in surface morphology and structure between CD34(+) cells from normal human subjects and from patients with leukemia. METHODS: Bone marrow mononuclear cells from 3 normal human subjects and 3 patients with M2b leukemia were collected by Percoll gradient centrifugation, followed by purification of CD34(+) cells by means of immunomagnetic bead separation (MiniMAC) and examination with flow cytometry. The morphology of the cells were then observed with optical and atomic force microscope (AFM) in air. RESULTS: No significant difference was identified between the two cells under optical microscope. With atomic force microscope, numerous microvilli were observed on the surface of CD34(+) cells, and the normal cells and those from the leukemic patients showed significant difference in terms of the roughness of the cell surface. CONCLUSION: Normal CD34(+) cells have more rough and erosive surface structure than leukemic CD34+ cells (M2b).

Antigens, CD34↗

PubMed2003

[Effect of ligustrazine on cell cycle and prostacyclin of injured vascular endothelial cells induced by hypoxia and lack of glucose].

OBJECTIVE: To study the effects of ligustrazine on cell cycle and prostacyclin of injured human umbilical vein vascular endothelial cell line induced by hypoxia and lack of glucose. METHODS: The experiments were performed by culturing vascular endothelial cell induced by hypoxia and lack of glucose in vitro. Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry. Radioimmunoassay (RIA) was used to assess the amount of prostacyclin (PGI2). RESULTS: The vascular endothelial cell viability and PGI2 were decreased in condition of hypoxia and lack of glucose-injury. Preincubation of vascular endothelial cell with Ligustrazine for 24 h significantly increased the cell viability, S-Phase cell, G2M-phase cell and PGI2 level. CONCLUSION: These results demonstrated that ligustrazine had protective effect on hypoxia and lack of glucose-injured vascular endothelial cell and the effect may be related to increasing cell viability, S-phase cell, G2M-phase cell and level of PGI2.

Cell Cycle↗

PubMed1999

AM1-SM2 Calculations Model the Redox Potential of Nitroxyl Radicals Such as TEMPO.

Nitroxyl radicals can be oxidized to N-oxo ammonium salts that are themselves useful oxidants for primary and secondary alcohols. Several computational methods were investigated in order to predict the redox potential of nitroxyl radicals and to better understand the behavior of different nitroxides as catalysts for alcohol oxidation. The difference in calculated heats of formation for N-oxo ammonium ions and nitroxyl radicals using AM1 did not lead to a useful correlation with experimental redox potential as measured by cyclic voltammetry. However, when both the N-oxo ammonium ion and the nitroxyl radical were evaluated using the Cramer-Truhlar solvation model (SM2), a linear correlation was observed between the difference in heats of formation and the experimental redox values. This correlation may be used to correctly predict the redox potential of new nitroxyl radicals.

Journal Article↗