Biomedical subjects

Rosario Tapia

Publications and source records attributed to Rosario Tapia.

3 recordsLinked to original sources

PubMed2003

[Progressive motility increase caused by L-arginine and polyamines in sperm from patients with idiopathic and diabetic asthenozoospermia].

This study was designed to investigate the in vitro effect of L-arginine and polyamines putrescine, spermidine, and spermine on sperm motility of asthenozoospermic idiopathic and diabetic patients. 10 semen samples were obtained from healthy male volunteers. Other 10 samples were from men with idiopathic asthenozoospermia and 13 samples were from men with diabetes mellitus type 1. In vitro effect of L-arginine and polyamines on sperm motility type a was studied. In addition, concentration of L-arginine and polyamine levels was measured in seminal plasma. Addition of L-arginine or polyamines to sperm cells suspensions resulted in significant differences in motility type a. L-arginine concentrations in seminal plasma were similar in the three groups. Putrescine and spermidine levels in seminal plasma of diabetic patients were lower than those of healthy patients (p < or = 0.003 and p < or = 0.004, respectively). In asthenozoospermic patients, only spermidine was lower (p < or = 0.009). We can conclude that under the conditions of this study, the addition of L-arginine and polyamines to human sperm cells obtained from idiopathic or diabetic asthenozoospermic patients showed a beneficial effect on sperm motility.

Adolescent↗

PubMed2002

Excessive erythrocytosis, chronic mountain sickness, and serum cobalt levels.

In a subset of high-altitude dwellers, the appropriate erythrocytotic response becomes excessive and can result in chronic mountain sickness. We studied men with (study group) and without excessive erythrocytosis (packed-cell volume >65%) living in Cerro de Pasco, Peru (altitude 4300 m), and compared them with controls living in Lima, Peru (at sea-level). Toxic serum cobalt concentrations were detected in 11 of 21 (52%) study participants with excessive erythrocytosis, but were undetectable in high altitude or sea-level controls. In the mining community of Cerro de Pasco, cobalt toxicity might be an important contributor to excessive erythrocytosis.

Adult↗

PubMedDate not supplied

Glu298Asp endothelial nitric oxide synthase polymorphism is a risk factor for erectile dysfunction in the Mexican Mestizo population.

Penile erection depends on the balanced action between antagonist vasoactive molecules such as nitric oxide (NO) and angiotensin. Endothelial nitric oxide synthase (eNOS) and angiotensin-converting enzyme (ACE) polymorphisms have been associated with endothelial dysfunction, which is described as a cause of erectile dysfunction (ED). Endothelial NOS and ACE are both regulators of vascular and corporal smooth muscle tone, which are connected by interaction between the NO-cyclic guanosine monophosphate pathway and the renin-angiotensin system. We analyzed the frequencies of 894 G/T (Glu298Asp) eNOS and ACE I/D polymorphisms in Mexican patients with ED (n=53) and in an age-matched control group (n=62). The populations analyzed were in Hardy Weinberg equilibrium. We found significant differences in allelic (chi2=4.42; P=.03) and genotypic frequencies (chi2=3.96; P=.04) between patients and controls for the 894 G/T eNOS polymorphism. Presence of the 894T allele in carriers increased the risk of ED (odds ratio [TT + GT versus GG] = 2.37; 95% confidence interval, 1.08 to 5.21; P=.02). Multiple logistic regression analysis showed that the Glu298Asp polymorphism was an independent factor for ED, as was diabetes mellitus, hypertension, cardiac disease, and cigarette smoking. No association was found between ACE I/D polymorphism and ED in the population studied. Therefore, our results suggest that Glu298Asp eNOS polymorphism plays a role as a genetic susceptibility factor for ED.

Adult↗