Biomedical subjects

Rui Liu

Publications and source records attributed to Rui Liu.

At least 55 records · Page 3Linked to original sources

PubMed2005

[Apoptosis of pulmonary epithelial cells and endothelial cells in mice exposed to phosgene].

OBJECTIVE: To study apoptosis of pulmonary epithelial cells and endothelial cells in mice with pulmonary edema induced by phosgene exposure. METHODS: Thirty-two mice were divided into normal group and phosgene group with 16 mice in each group. The mice in phosgene group were exposed to phosgene (11.9 mg/L) for 5 min and those in the control group to air. Four hours after exposure, alveolar type II cells were isolated and cultured to observe their apoptosis by electron microscope and flow cytometry. The lung tissues were also taken for DNA gel electrophoresis and TUNEL assay. RESULTS: Apoptotic bodies were observed in alveolar type II cells under electron microscope in phosgene group, which had higher cell apoptosis rate than the control group [(40.26+/-7.74)% vs (1.58+/-1.01)%, P<0.001] as determined by flow cytometry. Ladder-like DNA fragmentation pattern was observed in DNA gel electrophoresis in phosgene group with apoptosis of the pulmonary epithelial and endothelial cells observed by TUNEL. CONCLUSIONS: Phosgene can induce pulmonary epithelial and endothelial cell apoptosis in mice, suggesting that the mechanism of phosgene-induced pulmonary edema involves apoptosis of the lung cells.

Animals↗

PubMed2005

[Advances in research and application of water-treatment flocculants].

Water-treatment flocculants are being developed from traditional to inorganic, from inorganic to organic, from natural organic to synthetic organic, from synthetic organic to modified natural organic, from inorganic or organic to inorganic-organic, and from chemical to biological with ecological safety. In order to promote the rapid development and practical application of water-treatment flocculants, the research progress and application situation of flocculants for water treatment at home and abroad were reviewed. In particular, the characteristics of two important flocculant types, chemical and microbiological flocculants, as well as their application processes in water treatment were summarized, their development orientations were analyzed and evaluated, and the future research directions aimed to overcome the current shortages in developing and applying flocculants in China were put forward.

Ecosystem↗

PubMed2005

[Clinicopathological study of lymphatic vessel density and distribution in pancreatic cancer].

OBJECTIVE: To clarify the clinicopathological significance of lymphatic vessel density (LVD) and distribution in pancreatic cancer. METHODS: We measured LVD in 43 pancreatic cancer specimens by immunostaining with specific lymphatic endothelium marker, and examined their relationship with well-defined clinicopathological variables. RESULTS: Intratumoral LVD (9.4 +/- 10.0) was significantly lower than periturmoral (16.0 +/- 9.7) (P < 0.001) and nontumoral LVD (13.5 +/- 6.0) (P < 0.01). Increased peritumoral LVD correlated significantly with tumor staging (P < 0.05) and lymph node involvement (P < 0.05). CONCLUSION: The lymphatic vessels distribution in pancreatic cancer samples and peritumoral lymphangiogenesis may promote the malignant progression and lymph node metastasis of pancreatic cancer.

Adenocarcinoma↗

PubMed2005

[Reconstruction of mandibular defect caused by resection of oral carcinoma with pectoralis major myocutaneous flap and ti-plate system].

OBJECTIVE: To investigate the clinical effect of the pectoralis major myocutaneous flap and Ti-plate system in repairing mandibular defects caused by resection of oral carcinoma. METHODS: From November 2001 to February 2003, 32 patients with mandibular defect caused by resection of oral carcinoma were treated. Combined radical neck dissection with resection of gingival and mandible was performed on 11 patients with carcinoma of the lower gingival, combined radical neck dissection with glossectomy and mandibulectomy on 13 patients with carcinoma of tongue, combined radical neck dissection with resection of floor of mouth and mandible on 4 patients with carcinoma of floor of mouth, and combined radical neck dissection with resection of cheek and mandible on 4 patients with carcinoma of buccal mucosa, respectively. The defects of mandible were associated with soft-tissue component, the sizes of defect ranged from 5.5 cm x 7.6 cm to 8. 2 cm x 10.5 cm. The defects were reconstructed with 6 cm x 7 cm to 9 cm x 10 cm pectoralis major myocutaneous flaps and Ti-plate system. The effect was studied retrospectively. RESULTS: Thirty-two cases were followed for 2-19 months; 29 cases of flaps survived and 3 cases of flaps partly necrosed (10% or less of the skin paddle). The appearance of face was satisfactory in 27 patients, and slight deformity of face was observed in 5 patients. The occluding relation and masticatory function were recovered well. Opening mouth extents ranged from 2.7 cm to 3.4 cm. No temporomandibular arthrosis relating to operation was found in all cases. CONCLUSION: A combination of the pectoralis major myocutaneous flap and Ti-plate system is an ideal method for reconstruction of mandible defects associated with soft-tissue component after radical operation of oral carcinoma.

Adult↗

PubMed2005

[Characteristics and influencing factors of ammonia contamination from production of monosodium glutamate].

Fluxes of ammonia contamination from production of monosodium glutamate (MSG) were quantitatively observed and relationships with some important influencing factors were statistically analyzed. The study indicates that there is a big difference in the release of ammonia at each step of MSG production including sewage treatment, depending on technical processes. The flux of ammonia released in the process of fermentation was highest, up to 880 mg x (L x min)(-1), and that in the process of refining sugar was lowest, only 0.07 mg x (L x min)(-1). Fluxes of NH3 emission during the whole process of MSG production are significantly related with the concentration of ammonium-nitrogen (NH4+ -N) in discharged solution linearly, and with concentrations of nitrate-nitrogen (NO3- -N) including nitrite-nitrogen (NO2- -N) in discharged solution exponentially. Although there is no linear relationship between fluxes of NH3 emission during the whole process of MSG production and pH values in discharged solution, it is significantly related with fluxes of NH3 emission during the process of sewage treatment.

Air Pollutants↗

PubMed2005

[Identification of 5 constituents of the aqueous extract of Isatis indigotica by HPLC-MS2].

OBJECTIVE: To identify five constituents in the aqueous extract of Isatis indigotica Fort. METHODS: After separation of the aqueous extract of Isatis indigotica Fort. by HPLC, the eluates of five peaks were collected separatively and analysed by MS2. UV spectra and MS2 were compared with those of reference standards of cytidine, uridine, guanosine xanthine and hypoxanthine. RESULTS: Each HPLC elute of the aqueous extract had same retention time, UV spectra and fragment pattern in the MS2 spectrum as the corresponding standards. CONCLUSION: Five constituents of the aqueous extract of Isatis indigotica Fort. are identified as cytidine, hypoxanthine, uridine, xanthine and guanosine.

Chromatography, High Pressure Liquid↗

PubMed2004

Nicotinic receptor alpha subunits in magnocellular neurons of rat hypothalamus.

Mab35 is a monoclonal antibody against one specific immunogenic region in alpha1, alpha3, alpha5 subunits of nicotinic acetylcholine receptors (N-AChR) of a variety of species. It has previously been claimed that N-AChR-like immunoreactivity (-LI) identified by mab35 is present in vasopressin-containing magnocellular neurons. However, we show here by double immunofluorescence labelling that mab35 immunoreactivity is predominantly localized to oxytocinergic rather than vasopressinergic magnocellular neurons. We further infer that mab35 predominantly stained the alpha3 and/or alpha5 subunits in rat oxytocinergic neurons, and suggest that the unbalanced distribution of these subunits may contribute to some specific physiological properties of oxytocinergic neurons.

Animals↗

PubMed2004

First-principle predictions of absolute pKa's of organic acids in dimethyl sulfoxide solution.

MP2/6-311++G(d,p) and B3LYP/6-311++G(2df,p) methods were found to be able to predict the gas-phase acidities of various organic acids with a precision of 2.2 and 2.3 kcal/mol. A PCM cluster-continuum solvation method was developed that could predict the solvation free energies of various neutral, cationic, and anionic organic species in DMSO with a precision of about 2.0 kcal/mol. Using these carefully tested methods, we successfully predicted the pKa's of 105 organic acids in DMSO with a precision of 1.7-1.8 pKa units. We also predicted the pKa's of a variety of organosilanes in DMSO for the first time using the newly developed methods. This study was one of the first that employed first-principle methods for calculating pKa's of unrelated compounds in organic solutions.

Journal Article↗

PubMed2004

Serum high-density lipoprotein-cholesterol levels modify the association between plasma levels of oxidatively modified low-density lipoprotein and coronary artery disease in men.

We investigated the association among plasma levels of oxidatively modified low-density lipoprotein (Ox-LDL), high-density lipoprotein-cholesterol (HDL-C), and the prevalence of coronary artery disease (CAD) in a case-control study. Cases (n = 183, male [M]/female [F]:138/45, age: 64.9 +/- 10.6 years) were defined as patients with angiographically proven coronary atherosclerosis (>/=50% stenosis) and controls were subjects with normal coronary arteries (n = 74, M/F:36/38, age: 57.6 +/- 14.4 years). Plasma Ox-LDL levels were measured by a sensitive detection method using the monoclonal antibody DLH3. In women, both Ox-LDL and lipid variables were similar between cases and controls. In men, cases had significantly lower (P <.05) levels of HDL-C (39.1 +/- 10.3 v 42.8 +/- 10.9 mg/dL) and apolipoprotein (apo) A-I than controls, while the difference in Ox-LDL between cases and controls was not significant (1.05 +/- 0.79 and 0.83 +/- 0.65 ng/10 microg LDL protein, respectively). However, HDL-C levels interacted with the association between Ox-LDL levels and CAD in males: increased Ox-LDL levels were significantly associated with CAD after controlling for age when HDL-C levels were high, but were not associated with CAD when HDL-C levels were low, as assessed by a multiple logistic regression analysis. In addition, the combination of HDL-C and Ox-LDL levels was a better indicator for CAD in males than HDL-C levels alone (-2 log likelihood, 24.1 v 19.4) after controlling for age and conventional risk factors of CAD, while Ox-LDL levels were not significantly associated with CAD. HDL-C levels interact with the association between plasma Ox-LDL levels and CAD in men, and increased Ox-LDL levels are an indicator of CAD in male subjects with high HDL-C levels.

Adult↗

PubMed2004

Increased activity of the intestinal mucosal mast cells in rats with multiple organ failure.

OBJECTIVE: This study was undertaken to evaluate the changes in the activity of the intestinal mucosal mast cells (IMMC) in multiple organ failure (MOF). METHODS: A rat model of MOF was induced by intraperitoneal injection of zymosan. Both the functional alterations and the pathological morphology of essential organs, such as small intestine, liver, kidney and the lungs, were evaluated. The concentrations of histamine and tumor necrosis factor alpha (TNF-alpha) in plasma and small intestinal tissue were detected by fluorimetric assay and enzyme-linked immunosorbent assay, respectively. The ultrastructural changes of the IMMC from the MOF rats were evaluated by transmission electron microscopy. RESULTS: Zymosan induced obvious inflammatory morphology and functional impairment in the essential organs of the rats that were indicative of the characteristic changes in MOF. There was a significantly decreased concentration of histamine in the intestinal tissue of the MOF rats when compared with the normal controls (8.67 +/- 1.16 vs 11.63 +/- 1.97 ng/g protein, P < 0.01), whereas the plasma histamine concentration showed no significant changes. The concentration of TNF-alpha was elevated significantly in both the intestinal tissue and plasma of MOF rats. Furthermore, both the increased number and level of degranulation of the IMMC in the gut tissue was marked in MOF rats. CONCLUSION: The results suggest that the histamine and TNF-alpha released from activated IMMC play an important role in the development of MOF.

Animals↗

PubMed2004

[Imbalanced T cell-specific transcription factors T-bet and GATA-3 contributes to type 2 T helper cell polarization in asthmatic patients].

OBJECTIVE: To identify the T helper cell predominant differentiation in asthmatic patients and to explore the modulation of T cell-specific transcription factors T-bet/GATA-3. METHODS: Thirty-two asthmatic patients were enrolled, among whom 18 were atopic defined by positive antigen skin test and 12 were children. Lymphocytes were isolated from peripheral blood and incubated with PHA (100 microg/ml) at 37 degrees C for 48 hours. INF-gamma and IL-4 concentrations in the supernatant were detected by ELISA. The T-bet and GATA-3 mRNA expression levels in lymphocytes were assayed by reverse transcription-polymerase chain reaction (RT-PCR) while the ratio of CCR3+ and CCR5+ cells in lymphocytes was counted by flow cytometry (FCM) after direct immunofluorescence staining. RESULTS: IL-4 concentration in the lymphocyte supernatant of the asthmatic group was (118 +/- 25) microg/L, which was significantly elevated compared to that of the healthy control group (75 +/- 12) microg/L (P < 0.01). When subgroups of asthmatic patients were compared, the results showed that atopic subjects had a higher IL-4 level than non-atopic subjects [(126 +/- 23) microg/L vs (107 +/- 26) microg/L, P < 0.01], but no significant difference was demonstrated between adults and children [(118 +/- 25) micro g/L vs (121 +/- 25) microg/L, P > 0.05]. Significantly lower concentration of INF-gamma in the asthmatic group was detected as compared to the control [(651 +/- 85) microg/L vs (1 179 +/- 332) microg/L, P < 0.001]. The concentration of INF-gamma was higher in atopic subjects than in non-atopic subjects [(618 +/- 89) micro g/L vs (680 +/- 83) microg/L, P < 0.01], but no difference was found between adults and children. The percentage of CCR3+ cells in lymphocytes was (9.4 +/- 5.8)% in the asthmatic group and (4.9 +/- 2.3)% in the control (P < 0.05), while the percentages of CCR5+ cells was (6 +/- 7)% and (13 +/- 7)%, respectively (P < 0.05). RT-PCR revealed that T-bet mRNA expression levels were as follows: 0.13 +/- 0.03 in the asthmatic group and 0.18 +/- 0.04 in the control (P < 0.01); 0.120 +/- 0.030 in atopic subjects and 0.140 +/- 0.010 in the non-atopic subjects (P < 0.05); 0.120 +/- 0.020 in children and 0.130 +/- 0.020 in adults (P > 0.05). The levels of GATA-3 mRNA expression were 0.43 +/- 0.07 in asthma and 0.29 +/- 0.09 in the control (P < 0.01), however, no differences were found between atopic and non-atopic, children and adults (0.50 +/- 0.12 vs 0.40 +/- 0.10, 0.44 +/- 0.09 vs 0.43 +/- 0.07, respectively, P > 0.05). A positive correlation was found between concentration of INF-gamma and T-bet mRNA level (r=0.663, P < 0.01), while no correlation with GATA-3 mRNA expression was found. The concentration of IL-4 was negatively correlated with T-bet mRNA level (r=-0.250, P < 0.05) and positively with GATA-3 mRNA level (r=0.72, P < 0.01). It was interesting that a closer relationship existed between the ratio of T-bet to GATA-3 and the ratio of INF-gamma to IL-4 (r=0.873, P < 0.01). CONCLUSIONS: In asthma there is a tendency of Th2 polarization with over-production of Th2-like cytokines in which T-bet deficiency may be a key factor. T-bet might direct T cells to Th1 differentiation while GATA-3 orientated Th2 maturation. Considering the fact that committed Th2 cells underwent re-differentiation induced by T-bet, this novel Th1-specific transcription factor is a fascinating target gene for modifying to restore the Th1 and Th2 balance.

Adolescent↗

PubMed2004

[Effect of acute phosgene inhalation on antioxidant enzymes, nitric oxide and nitric-oxide synthase in rats].

OBJECTIVE: To study the effect of acute phosgene inhalation on the antioxidant enzymes, nitric oxide (NO) and nitric oxide synthase (NOS) in rats. METHODS: Phosgene was produced by decomposing bis (trichdomethyl) carbonate in the presence of N,N-dimethyl formamide. SD rats were randomly divided into two groups: control and phosgene exposure groups. In a special experimental device with equipment modulating the gas flow, phosgene exposed rats inhaled phosgene quantitatively for five minutes. Two hours later, all the rats were sacrificed and the ratio of wet weight to dried weight of lung (WW/DW) was calculated. Peripheral blood, serum and liver were collected to examine the activities of antioxidant enzymes including glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), NOS, and NO level. The total content of proteins were also determined. RESULTS: The WW/DW ratio of lung in phosgene exposure group was much higher than that in control group (P < 0.01). The activities of GST in serum and liver of phosgene exposure group increased significantly (P < 0.05). The activities of SOD, CAT, GSHPx and NOS in serum or blood and liver of phosgene exposure group were also increased significantly (P < 0.05). But the content of NO was significantly decreased (P < 0.01). CONCLUSION: Acute phosgene inhalation may cause a dramatically changes of several antioxidant enzyme activities, and acute injury of liver to some extent in rats. The latter is related to reactive oxygen species. But the elevation of antioxidant enzyme activities suggests that antioxidative treatment for acute phosgene poisoning should not be considered first.

Animals↗

PubMed2004

[Atherogenic index of plasma is associated with carotid intima-media thickness in patients with type 2 diabetes mellitus].

OBJECTIVE: To investigate the relationship of atherogenic index of plasma (AIP), an indirect indicator of LDL particle size, to carotid intima-media thickness (IMT) in patients with type 2 diabetes mellitus (T2DM) among Chinese Han nationality population. METHODS: One hundred and seventy-nine type 2 diabetic patients and 87 healthy subjects from a population of Chinese Han nationality were included. The AIP is the logarithmically transformed ratio of TG/HDL-c, which may serve as a surrogate method for assessing LDL particle size. The carotid IMT, as a surrogate continuous variable for macroangiopathy, was measured by B-mode ultrasonography in type 2 diabetic patients. The patients with T2DM were divided into three subgroups according to the IMT: IMT < or = 0.65 mm, 0.65 mm < IMT < or = 1.1 mm and IMT > 1.1 mm. The Student-t test, one-way analysis of variance, univariate and multivariate analyses were implemented. RESULTS: (1) In T2DM group the values of triglyeeride (TG) and AIP were significantly higher than those in control group respectively, and the HDL-c value of T2DM group was significantly lower than that of control group. (2) There was significant difference in the age, body mass index (BMI), waist-hip ratio (WHR), the duration of DM, triglyceride (TG), total cholesterol (TC), LDL-c, HDL-c, HbAlc, TG/HDL-c and AIP among the three subgroups; the HDL-c value was inversely related to the IMT of T2DM, and all the other risk factors were positively related to the IMT of T2DM. (3) Multivariate analysis by linear regression showed that the value of AIP was an important risk factor in the pathogenesis and progress of IMT in patients with T2DM. CONCLUSION: AIP value, an indirect indicator of LDL particle size, is an important risk factor in pathogenesis and progress of IMT in patients with T2DM.

Adult↗

PubMed2004

[Inhibitory effect of quercetin, rutin and puerarin on HDL oxidation induced by Cu2+].

OBJECTIVE: To evaluate the inhibitory effect of quercetin, rutin and puerarin on the HDL oxidation induced by Cu2+ and to investigate their action on the prevention and cure of atherosclerosis. METHODS: The serum HDL of healthy human was isolated by the one step density gradient ultracentrifugation. The HDL oxidation was induced by Cu2+ in vitro for different time, quercetin and rutin at 5 micromol/L were added ahead, respectively. HDL was oxidized, then identified by A234, REM, TBARS and protein carbonyls content, and was compared with control group. Inhibition of puerarin was weak. RESULTS: (1) A234, REM, TBARS and protein carbonyls formation increased gradually during HDL oxidation induced by Cu2+ in vitro. (2) During HDL oxidation induced by Cu2+ in vitro and incubation with quercetin and rutin, the kinetic changes of A234, REM, TBARS and protein carbonyls formation showed lag phases of 2 h, 2-4 h, 2-4 h and 4 h respectively, and showed reductions by 24.2%-49.0% (P<0.001) and 9.4%-42.0% (P<0.01); 3.0%-25.0% and 4.0%-20.7% (P<0.05); 7.9%-34.1% and 1.2%-26.8% (P<0.05); 28.3%-50.4% and 18.6%-64.6% (P<0.001) respectively. In the puerarin group, only protein carbonyls formation showed a lag phase of 4 h and a reduction by 14.1%-52.1% (P<0.001) while its A234, REM, TBARS reduced to a small extent. CONCLUSION: The above findings suggested that quercetin and rutin inhibit oxidation of HDL significantly, but puerarin has less antioxidative function.

Antioxidants↗

PubMed2003

Gln-Arg192 polymorphism of paraoxonase 1 is associated with carotid intima-media thickness in patients of type 2 diabetes mellitus of Chinese.

Serum paraoxonase (PON) is a high-density lipoprotein-bound enzyme that can prevent oxidation of low-density lipoprotein by hydrolyzing lipid peroxides and thus exert an anti-atherogenic effect. Recent studies have suggested that glutamine(Q isoform)/arginine(R isoform) polymorphism at position 192 of PON(1) gene is associated with macrovascular disease of type 2 diabetes mellitus (T2DM). We re-investigated this relationship using carotid intima-media thickness (IMT) as a surrogate continuous variable for macroangiopathy. The genotype and allele frequency of PON(1) 192 Q/R polymorphism was assayed by polymerase chain reaction-restriction fragment length polymorphism in 152 type 2 diabetic patients and 128 healthy subjects from a population of Chinese Han nationality in ChengDu area. The carotid IMT was measured by B-mode ultrasonography in type 2 diabetic patients. No differences were found in PON(1) gene Q/R polymorphism in the type 2 diabetic patients when compared with the control group. The mean carotid IMT in type 2 diabetic subjects with the QQ, QR and RR genotype was 0.65+/-0.27, 0.83+/-0.27 and 1.05+/-0.32 mm, respectively. One-way ANOVA showed that IMT was significantly greater in the RR subgroup than in both QR and QQ subgroups (P<0.01). Multivariate logistic regression analysis showed R allele to be the main determinant of IMT variability (OR 4.0 95% CI 2.10-7.40 P=0.005). Our data support the view that 192 R allele of PON(1) gene is a risk factor for macrovascular disease of T2DM in Chinese.

Amino Acid Substitution↗

PubMed2003

[Molecular scanning of candidate mtDNA gene fragment in diabetic pedigrees].

OBJECTIVE: To explore novel pathogenic mutation in the mitochondrial DNA gene in diabetic pedigree. METHODS: Twenty-eight suspected mitochondrial DNA diabetic families were recruited. The gene fragment was produced by PCR, and mutation was detected by direct sequencing. RESULTS: In one pedigree, the proband and her mother were found carrying the most common nt3243 A --> G mutation and another 16S rRNA 3205C --> T mutation. But only 3205C --> T was found in her affected brother. All the two patients were deaf and developed diabetes in early age, characterized by impaired beta cell function and low body mass index (BMI). The proband had relatively higher lactic acid concentration than normal individuals. A novel ND1 gene 3434 A --> G(TAT --> TGT) mutation was explored in another proband with deafness and her affected family members. CONCLUSION: 16SrRNA 3205C --> T mutation was found in a mitochondrial diabetes mellitus pedigree, implying its potential pathogenic role in diabetes. Another novel ND1 3434 A --> G mutation was found in another diabetic pedigree. Because this mutation causes amino acid change (Tyr --> Cys) and is co-segregated with diabetes, it may be diabetogenic.

Adult↗

PubMed2003

[Clinical significance of regional lymphadenectomy in radical resection of ductal adenocarcinoma in the pancreatic head].

OBJECTIVE: To prospectively evaluate the long-term effect of pancreaticoduodenectomy with regional lymphadenectomy. METHODS: One hundred and twenty-one patients with ductal adenocarcinoma in the pancreatic head treated from 1996 to 2001 were studied prospectively. The enrollment of the patients was dependent on 7 criteria. The patients were divided into two groups: regional lymphadenectomy (group A, n = 50) and routine Whipple procedure (group B, n = 71). Their pre- and postoperative conditions, clinicopathological data, survival rates were studied. RESULTS: It was comparable between the 2 groups in age, sex, preoperative risk factors, operative management, and postoperative complication. Clinicopathological results showed no difference in tumor size and plexus invasion; but the frequency of lymph node involvement and the amount of resected lymph node in group A were significantly higher than those in group B. The rate of local recurrence was significantly higher in group A than in group B. The survival rates of 1-, 3-, 5-year in group A were 70.8%, 31.4%, 20.9%, respectively, which were higher than those in group B. No direct relations were observed between nodal involvement and survival rate. CONCLUSION: Lymphadenectomy in radical pancreaticoduodenectomy could remove lymph nodes effectively and sufficiently, and reduce the rate of local recurrence so as to improve the long-term survival rate.

Adult↗

PubMed2003

[Polymorphism of insulin receptor substrate-1 gene in patients with coronary heart diseases].

OBJECTIVE: To explore the relationship between the polymorphism of insulin receptor substrate-1 (IRS-1) gene and coronary heart diseases in Chinese population. METHODS: A total of 113 patients with coronary heart diseases and 194 healthy Chengdu residents with less than 82 mmol/L of TG and 6.2 mmol/L of TC in fasting serum testing were selected and compared. The genotype and allele frequency of IRS-1 gene polymorphism was assayed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). The serum lipids were measured by enzymatic kits. RESULTS: The GG genotype dominated in the IRS-1 gene with frequency of 0.960 and 0.985 for patients and healthy controls respectively. Difference appeared in R allele frequency (0.015 for healthy controls and 0.040 for patients, P = 0.05). Patients with genotype G/R had significantly higher level of serum TC and lower level of plasma glucose than patients with genotype G/G (P < 0.05). CONCLUSION: IRS-1 gene polymorphism is associated with coronary heart disease in Chinese population. The G/R and G/G genotype of IRS-1 gene contributes to the increasing of serum cholesterol and plasma glucose respectively.

Aged↗