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Biomedical subjects

S A Alexander

Publications and source records attributed to S A Alexander.

At least 55 records · Page 3Linked to original sources
PubMed1980

Effect of fixation upon the histochemistry of glycosaminoglycans (mucopolysaccharides) in intraoral wounds.

Intraoral wounds and biochemical films were examined to determine the best method of fixation for glycosaminoglycans. Tissues fixed in 1% cetyl pyridinium chloride-10% formalin stained intensely with preparations of Alcian blue. When these tissues were treated with Streptomyces hyaluronidase and bacterial chondroitinases, staining was comparable to untreated tissues fixed in only 10% formalin. The addition of cetyl pyridinium chloride to formalin fixatives was a requirement for glycosaminoglycan histochemistry in open wounds, and presumably in other tissues that contain these complex sugars.

Alcian Blue↗
PubMed1980

Mucopolysaccharidase activity in traumatized human deciduous teeth undergoing accelerated resorption: isolation and characterization.

Mucopolysaccharidase activity was observed in traumatized human decidous teeth. Histochemical analysis of the periodontal ligaments from these teeth revealed a loss of film substrate metachromasia during incubation, indicating enzyme activity. Routine histology of these ligaments showed the presence of an inflammatory infiltrate throughout this tissue. Biochemical analysis of the ligaments revealed a 10-fold increase of enzyme activity when incubation time was increased from 1 to 8 h. When compared to the enzyme activity measured during physiologic resorption, activity was increased. This suggests that the presence of an inflammatory infiltrate not observed in the tissues undergoing physiologic resorption may be responsible for the rapid resorption seen in traumatized deciduous teeth.

Child↗
PubMed1980

Identification and localization of a mucopolysaccharidase in human deciduous teeth.

The presence of a mucopolysaccharidase in resorbing deciduous teeth was investigated using histochemical techniques. The loss of toluidine blue metachromasia within glycosaminoglycan film substrates indicated the presence of enzyme activity, and was related to physiologic resorption. Such activity was localized to the periodontal ligament of these teeth.

Adolescent↗
PubMed1980

Dental pulp mucopolysaccharidase: identification and role in tooth resorption.

The presence of mucopolysaccharidase activity within the pulps of resorbing deciduous teeth was investigated using histochemical techniques. The loss of toluidine blue metachromasia within glycosaminoglycan film subtrates indicated the presence of enzyme activity. This was related to physiologic resorption.

Adolescent↗
PubMed1980

Elastic tissue in early intraoral wounds.

Histochemical analysis of elastic tissue in early intraoral healing of wounds demonstrated that these fibers were concentrated in the perivascular areas of granulation tissue and within newly formed connective tissues at the base of the wound. These results were consistent with previous reports indicating that the cellular origin of this tissue is smooth muscle cells or fibroblasts.

Animals↗
PubMed1980

Patterns of cell polarity in healing open wounds.

The fibroblasts of the open wound, and especially those of the whole edge, are characteristically elongated, with their long axes parallel to the connective tissue bundles. Their cytoplasmic organelles are polarized, suggesting that secretory products are released from one end of the cell. Results of the current study indicate that orientation and polarization of granulation tissue fibroblasts may be coupled with secretion of collagen and glycosaminoglycan during early wound healing.

Animals↗