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Biomedical subjects

S A Goedegebuure

Publications and source records attributed to S A Goedegebuure.

At least 19 recordsLinked to original sources
PubMed1999

A polymerase chain reaction screening strategy for the promoter of the canine dystrophin gene.

OBJECTIVE: To develop a polymerase chain reaction (PCR) strategy to screen the dystrophin promoter(s) in dogs with cardiac and skeletal myopathies. ANIMALS: 9 Doberman Pinschers, 1 Dalmation, and 1 Saint Bernard with dilated cardiomyopathy (DCM); 1 Irish Terrier with muscular dystrophy; and 2 dystrophin-deficient German Shorthaired Pointers (GSHP). PROCEDURE: For each of the 3 unique exons associated with the muscle (M), Purkinje (P), and cortical (C) promoters of the dystrophin gene, each first exon, and the M promoter plus its first exon, were amplified, cloned, and sequenced. The M dystrophin transcript was amplified by reverse transcriptase PCR from skeletal and cardiac muscle RNA of 1 Doberman Pinscher and from skeletal muscle RNA of 1 GSHP. RESULTS: The M, P, and C first exons were amplified from all dogs except the 2 GSHP, which had a deletion encompassing the entire M, P, and C dystrophin promoter region. The M transcript could not be amplified from muscles of the GSHP, but was amplified from skeletal and cardiac muscle of the Doberman Pinscher. Sequencing of the product representing the M promoter and its first exon revealed no differences between clinically normal dogs and the Doberman Pinscher with DCM. CONCLUSIONS AND CLINICAL RELEVANCE: We have ruled out a major rearrangement of the dystrophin promoter region as the universal cause of DCM in Doberman Pinschers or of Irish Terrier myopathy. Use of the strategy identified a large deletion of this region in muscle from the GSHP.

Animals↗
PubMed1997

P53 gene mutations in osteosarcomas in the dog.

Osteosarcomas in 18 dogs were examined for the presence of p53 mutations in exons 4-8 by single strand conformation polymorphism (SSCP) analysis, followed by sequence analysis in tumors demonstrating abnormal bands in the SSCP analysis. P53 mutations were found in four of the primary tumors in 17 dogs. Metastases studied in two of these dogs in which the primary tumor contained only wild type p53 did not contain mutations, nor those of one dog in which the primary tumor was not studied. The alterations that were found included three missense mutations and one 3 bp insertion.

Animals↗
PubMed1997

Progressive neuronopathy in two Cairn terrier litter mates.

Clinical, histopathological, and EM findings are described for two Cairn terrier litter mates, an 18-months-old male and an 11-month-old female with progressive neuronopathy. The initial clinical signs were characterized by hind limb weakness and ataxia, which deteriorated with exercise. These signs progressed over several months to tetraparesis. Pathological examination revealed extensive chromatolytic degeneration of neurons and moderate secondary Wallerian-type degeneration in the spinal cord and brain stem. Progressive neuronopathy can be differentiated clinically from globoid cell leukodystrophy, another progressive neurological disorder in Cairn terriers, by the exercise-induced deterioration of the neurological signs. Progressive neuronopathy occurs only in Cairn terriers and because of the similarity in age of onset and the occurrence in one litter, an inherited disease is suspected.

Animals↗
PubMed1994

Flaccid trunk paralysis in free-ranging elephants (Loxodonta africana) in Zimbabwe.

An epizootic of flaccid trunk paralysis began in free-ranging Zimbabwean elephants (Loxodonta africana) on the southern shore of Lake Kariba in 1989. It involved a selective neuropathy of peripheral nerves supplying the trunk, with axon and myelin degeneration, muscle atrophy, compensatory hypertrophy, and fine endomyseal fibrosis, without inflammatory changes.

Animals↗
PubMed1993

Electromyography of the pelvic diaphragm and anal sphincter in dogs with perineal hernia.

The innervation of the levator ani and coccygeal muscles and the external anal sphincter was studied by anatomic dissection in 6 clinically normal male dogs and by electrical stimulation in 5 clinically normal male dogs. Variations in innervation occasionally were found that were comparable to those reported in previous studies. Electromyographic recordings were made from the levator ani and coccygeal muscles and from the anal sphincter in 40 dogs during perineal hernia repair. Spontaneous potentials of 4 types were found in 35 dogs: fibrillation potentials, positive sharp waves, complex repetitive discharges, and fasciculations. Biopsy specimens of the cranial part of the levator ani muscle were taken in 12 dogs during perineal hernia repair. Histologic examination revealed atrophy in 7 specimens. Spontaneous potentials were recorded from all muscles with histologic evidence of atrophy. All examinations of the levator ani muscle concerned the cranial part of this muscle, because the caudal part was absent in all 40 dogs. From combined results of electromyography and histologic examination, it was concluded that atrophy of the muscles of the pelvic diaphragm, which develops in some dogs with perineal hernia, is likely to be of neurogenic origin. Nerve damage is localized in the sacral plexus proximal to the muscular branches of the pudendal nerve or in the muscular branches separately.

Anal Canal↗
PubMed1992

Hepatic lipidosis in pregnant cows on a dairy farm.

A syndrome very similar to hepatic lipidosis is described in dairy cows during the dry period. After being sent to pasture the animals did not eat well for undetermined reasons. The disease phenomena were mainly observed in animals carrying twins. At post mortem examination severe falty infiltration was found in the 3 animals made available for post mortem examination. Increase of the energy supply to the dry cows by addition of maize silage to the ration prevented new cases.

Animals↗
PubMed1991

Dysphagia in Bouviers associated with muscular dystrophy; evaluation of 24 cases.

Twenty-four Bouviers with dysphagia were examined between October 1986 and October 1988. The type of dysphagia was characterised by the results from the clinical examination, the videofluorographic examination and the electromyographic recordings from the oral, pharyngeal, and esophageal muscles. Electromyography indicated neurogenic as well as myogenic causes of dysphagia. Tissues from 10 dogs were available for histopathologic examination. In nine dogs there was a progressive muscular degeneration of the pharyngeal and/or esophageal muscles, resembling muscular dystrophy. In two of these dogs the same abnormalities were also noticed in the masseter and temporalis muscles and in the intrinsic laryngeal muscles. In one dog small areas with hyalin degeneration and fragmentation of muscle fibres were found in the cricopharyngeal muscle. No abnormalities in nerve tissue were found. Muscular dystrophy is a hereditary disease. The mode of transmission in these Bouviers is not yet known.

Animals↗
PubMed1991

Growth and skeletal development in Great Dane pups fed different levels of protein intake.

Feeding a dog of a large breed with a diet exceeding the National Research Council (1974) recommendations for energy, protein, calcium, phosphorus and vitamin D may result in disturbances of skeletal development. The effects of excess energy and various calcium:phosphorous ratios per se have been reported by others. The role of dietary protein, especially with regard to calcium metabolism and skeletal development, in large breed-dogs is reported in this article. Seventeen Great Dane pups, 7 wk of age, were divided into three groups. During 18 wk each group received isoenergetic dry food (approximately 15 kJ metabolizable energy/g) containing 31.6, 23.1 or 14.6% protein on dry matter basis. No differences were found among the high (H-Pr), normal (N-Pr) and low protein (L-Pr) groups for the height at the shoulder. Significant differences were found between the H-Pr and L-Pr groups for body weight and plasma albumin and among all three groups for plasma urea. The differences in protein intake per se had no demonstrable consequences for calcium metabolism and skeletal development. A causative role for dietary protein in the development of osteochondrosis in dogs is unlikely.

Animal Feed↗
PubMed1989

Intravenous catheterisation of conventional pigs without application of antimicrobial agents.

In six experiments 43 castrated male conventional pigs weighing 25-41 kg were catheterised by inserting a cannula via the jugular vein into the cranal caval vein. The catheters were taped to the spinal neck region where the tap stops were located. Antimicrobial agents were not applied. One pig died 32 hour after surgery from Porcine Stress Syndrome. The catheters remained patent for at least nine days in 38 of the remaining 42 animals (90%). In two animals the catheter by mistake was not inserted into the jugular vein. Two animals got catheters with a one-way blockage four days after surgery. In these animals autopsy revealed thrombosis and phlebitis of the occluded vein and a valve-like thrombus at the tip of the catheters. In seven of the 43 pigs the effects of anaesthesia, surgery and catherisation were followed using rectal temperature and haematological and some blood biochemical parameters for nine days after the surgery. It is concluded that this catheterisation technique, without application of antimicrobial agents, can be used well for experimental infections and pharmacokinetic studies.

Animals↗
PubMed1989

The arterial supply of the navicular bone in the normal horse.

An arteriographic and histological study of the arterial blood supply of 35 navicular bones, from 19 adult horses with no clinical or radiographical signs of navicular disease, is described. The arteries enter the navicular bone from four directions: distal, proximal, medial, and lateral. The navicular bone can be divided into areas which receive arteries from one, two, or three directions, whereas the distal supply covers the largest part of the navicular bone. The distal arteries enter the navicular bone through the nutrient foramen which contains loose connective tissue, nutrient vessels and myelinated nerves. At least 77 per cent of the nutrient foramina contains synovial membrane. Twelve per cent of the arteries in the navicular bone show histological changes. Thickening of the intima with or without splitting of the internal elastic membrane and hyalinization, fibrosis and hypertrophy of the media were observed.

Angiography↗
PubMed1989

Development of the navicular bone in foetal and young horses, including the arterial supply.

A macroscopic, arteriographic and histological study of the development and the arterial anatomy of the navicular bone of 33 foetuses and 55 young horses is described. After 125 days of gestation the blood supply consists of two routes: one situated in the superficial layer of the fibrocartilage and the other similar to the blood supply of the navicular bone of the normal mature horse. After 270 days gestation, the blood vessels in the fibrocartilage gradually regressed and retracted until they have disappeared at six months after birth. At two months after birth the first macroscopic thinning of the fibrocartilage was noticed. From seven months to one year about 45 per cent of the navicular bones showed a slight thinning of the fibrocartilage. A positive correlation was found between radiographic abnormalities (ie enlargement of the nutrient foramina) and the frequency of thinning of the fibrocartilage. Radiographic abnormalities were first recognised 14 days after birth, whereas the arteriogram showed the first changes such as fewer or no arteries entering distally at the distal extremities at 10 weeks after birth. At four weeks after birth the first arterial wall changes were found, ie intimal thickening with or without splitting of the internal elastic membrane. From that age onward, the number of navicular bones with arterial wall changes gradually increased. Starting at five months after birth only 6 to 20 per cent of the arteries in the navicular bones without radiographic abnormalities showed arterial wall changes. However, the navicular bones with radiographic abnormalities showed arterial wall changes in 25 to 80 per cent of the arteries.

Angiography↗
PubMed1989

The effect of unilateral resection of segments of both palmar digital arteries on the navicular bone in ponies: an experimental study.

Clinical, radiographical, scintigraphical and histological effects on the navicular bone after resection of the medial and lateral palmar digital arteries in the pastern of one forelimb in ponies are evaluated. The acute disruption of the main blood supply of the distal extremity causes lameness due to the suddenness of the resection in which the collateral circulation is insufficiently developed. Because of the compensatory collateral circulation, no permanent symptoms of ischaemia occur despite the partial resection of both palmar digital arteries. Histologically the percentage of relative osteoid volume of the operated limb was significantly higher than in the control limb.

Angiography↗
PubMed1989

The arterial supply of the navicular bone in adult horses with navicular disease.

A macroscopical, arteriographical and histological study was made of the arterial supply of the navicular bones of horses with clinical and/or radiographic signs of navicular disease. Based on the clinical and radiological findings the navicular bones of 40 Warmblood horses are divided into four different groups. In the pattern of the arteriogram obvious changes are noticed: an imbalance and a distal to proximal shift between the distal and proximal arterial supply occur, indicating a reduction of the distal blood supply with a compensatory reaction of the proximal, medial and lateral supply. The changes in the pattern of the arteriogram are histologically defined by arterio(lo) sclerosis and newly formed arteries. The presence of radiologically visible nutrient foramina is associated with a changed pattern in the arteriogram, increased bone remodelling and fibrosis. Ischaemia and increased pressure (hypertension and/or increased intra-articular pressure) are considered to be responsible for changes in form and number of radiologically visible nutrient foramina.

Angiography↗
PubMed1989

The effect of artificial occlusion of the Ramus navicularis and its branching arteries on the navicular bone in horses: an experimental study.

The clinical, radiographic, arteriographic, scintigraphic and histological effects of experimental occlusion of the Ramus navicularis (R. Navicularis) and its branching arteries are evaluated. Occlusion of the R. navicularis and its branching arteries creates changes, arteriographically and histologically, which resemble those of navicular disease. The increased bone remodelling, the shift in arterial pattern, the formation of collaterals and the increased connective tissue in the synovial membrane and nutrient foramina, as a reaction to the reduction of the distal arterial supply of the navicular bone, are also pathological features in the navicular bones of horses with navicular disease. The results of the present study support the theory that a reduced distal arterial supply of the navicular bone is important in the pathogenesis of navicular disease.

Angiography↗
PubMed1988

The site of focal osteomyelitis lesions in foals.

The long bone ends of foals with infectious disease were sawn into sagittal slabs, washed and inspected. Tissue suspected to be abnormal on the basis of change of colour and consistency was radiographed, and then decalcified and examined histologically. The exact site of 140 focal osteomyelitis lesions from 18 foals was determined. There were more lesions in the epiphysis than the metaphysis. Epiphyseal lesions were in specific sites within a given epiphysis, and in most epiphyses the lesions were at the site of thickest cartilage. Metaphyseal lesions in the metacarpal, metatarsal and distal radial growth plates tended to be on the more inclined parts of the growth plate.

Animals↗
PubMed1988

Effects of divergent selection for leg weakness on angularity of joints in Duroc swine.

Sixty Duroc pigs, representing offspring of three lines from the fifth generation of divergent selection for leg weakness, were examined to determine correlated responses in joint angularity. The lines were low, control, and high, with the latter having superior front leg structure. At approximately 100 kg, 10 pigs of each sex and line were scored for front and rear leg structure and movement. The shoulder, elbow, carpal and hock joints were measured for resting angles and range of motion. The model to analyze the data included the effects of line, sire, sex and side and covariable for weight. High-line pigs had significantly smaller (P less than .05) resting angles of the elbow joint than did control- or low-line pigs. The low-line pigs, however, had significantly smaller resting angles at the carpal joint (P less than .01) and greater resting angles at the hock joint (P less than .05) than did control- or high-line pigs. The low-line pigs had fewer degrees extension at the elbow joint and fewer degrees flexion at the carpal joint than did control- or high-line pigs. High-line pigs had fewer degrees of flexion of the elbow joint than did control-or low-line pigs. Resting angles and range of motion for the elbow and carpal joints were less on the left side than on the right side. Males had greater degrees of extension and total degrees of movement at the elbow joint than did females. Selection for different degrees of leg weakness resulted in accompanying alterations in angularity of joints.

Animals↗
PubMed1987

Spontaneous primary myopathies in domestic mammals: a review.

By reviewing the literature concerning primary myopathies in man and animals, a classification of spontaneous primary myopathies in domestic mammals is made. This classification is based on aetiological factors. Primary myopathies are divided into metabolic, immunologic, toxic, nutritional, congenital, inherited, and inflammatory myopathies. Muscular dystrophies, in accordance with the definition in human cases, are considered as a separate entity. In the description of the different muscle disorders the emphasis is on the structural changes and the pathogenesis. Clinical signs and diagnostic criteria are considered only briefly.

Animals↗