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S A Isbell

Publications and source records attributed to S A Isbell.

4 recordsLinked to original sources
PubMed1997

Development of a protocol for the quantitative evaluation of contrast in NMR images of cryoprotective solvents in intact tissues.

In a recent study (Fyfe et al., Magn. Res. Chem. 32, 1994), chemical shift selective nuclear magnetic resonance (NMR) imaging was used to monitor the location and concentration of water and Me2SO in test samples. This study verifies that the technique can be applied to quantification of Me2SO in rat kidney and liver at temperatures commonly used for introducing cryoprotectants into tissues. A brief practical guide is provided for cryobiologists interested in using NMR imaging for such studies.

Animals↗
PubMed1997

Measurement of cryoprotective solvent penetration into intact organ tissues using high-field NMR microimaging.

Existing methods are not able to monitor accurately the penetration of cryoprotective solvents (CPS) into intact tissues. In this study, NMR imaging is shown to be a noninvasive nondestructive way to measure penetration rates and effective diffusion coefficients of Me2SO into samples of rat kidney and rat liver tissues. This new method is unique in that the measurements obtained are not averaged over the entire tissue volume but may be made at any site in the tissue. Measurements of penetration rates yield values which are similar to literature values, and the effective diffusion coefficients fall within the expected range. The images also suggest an explanation of why CPSs fail to completely protect organs from freezing damage.

Animals↗