Biomedical subjects

S A Khomenkova

Publications and source records attributed to S A Khomenkova.

5 recordsLinked to original sources

PubMed1991

[Tissue typing using a Soviet automated luminescent microscope-photometer].

Automated fluorescent microscope developed by the authors permits photometry of microquantities of cellular suspensions in scanning standard 60-well plates with a flat bottom. Automated and semiautomated modes of operation are possible. Fluorescent stains and schemes of staining the examined cells that yield stable results in the lymphocytotoxic test have been selected. Comparative analysis of the efficacies of histocompatibility antigens detection by the routine and the fluorescent technique has shown a number of advantages of the fluorescent method. Specific features of fluorescent stains used for staining live cells are described.

Histocompatibility Testing↗

PubMed1989

[A photographic microfluorimetric method for determining the DNA content in individual human chromosomes].

A method for determination of DNA contents in individual human chromosomes has been elaborated based of the two step analysis: 1) identification of chromosomes by Q-banding; 2) photographic microfluorimetry of chromosomes after the Feulgen staining (a fluorescent variant using a Schiff-type reagent of Auramine-SO2). The DNA content in 24 human chromosomes was calculated in absolute values (fg). The data obtained are compared with the evidence published elsewhere and provided by different cytophotometric methods.

Animals↗

PubMed1976

[Recording of azimuthal characteristics of the polarized fluorescence of microstructures using a semi-wave plate].

We have suggested and grounded theoretically application of lambda/2 plate for the registration azimuth characteristics (AC) of polarized fluorescence of microstructures. Proper device of polarization microfluorimeter has been worked out. Application of this device in case of transmitted light illumination has allowed to record AC of a very small objects (up to 1 mkm) and add to a-curacity of measurements. In the case of incident light illumination one need to make achromatic lambda/2 plates.

Spectrometry, Fluorescence↗

PubMedDate not supplied

[Experimental endofluorimeters for evaluating the intensity of luminescence of tissues in normal and pathological conditions during endoscopic studies and surgical interventions].

Various analogs of devices recording the fluorescence intensity of tissues directly in the body chambers (fluorescent endoscopes/photometers, microfluorometers for investigation of biopsies and intraoperative probes) are described.

Endoscopes↗

PubMedDate not supplied

[Optimized recording of results of the lymphocytotoxicity test using a luminescent attachment to Biolam-P-I microscope].

The fluorescent attachment appreciably simplifies the procedure of recording the results of the lymphocytotoxic test, is compact, economic, and easily inserted in the Biolam-P-I inverted microscope. Use of fluorescent stains helps selectively stain viable and intoxicated cells, thus simplifying their estimation. The said modification may be used in HLA typing and assessment of the principal lymphocyte subpopulations in the peripheral blood.

B-Lymphocyte Subsets↗